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Insights into the effects of acetate on the community structure of Candidatus Accumulibacter in biological phosphorus removal system using DNA stable-isotope probing (DNA-SIP)

Enzyme and Microbial Technology, 2020
Sodium acetate has been most commonly used as the external carbon source to achieve successful performance of full-scale enhanced biological phosphorus removal (EBPR) processes, but its microbial mechanism for the improvement of phosphorus removal performance was still unclear.
Baogui Wang   +6 more
openaire   +2 more sources

Comparison of methods for simultaneous identification of bacterial species and determination of metabolic activity by protein‐based stable isotope probing (Protein‐SIP) experiments

Rapid Communications in Mass Spectrometry, 2009
Abstract We developed a concept for analysing carbon and nitrogen fluxes in microbial communities by employing protein‐based stable isotope probing (Protein‐SIP) in metabolic labelling experiments with stable isotope labelled substrates. For identification of microbial species intact protein profiling (IPP) can be
Nico, Jehmlich   +6 more
openaire   +2 more sources

Carbon-13 (13C) Labeling of Bacillus subtilis Vegetative Cells and Spores: Suitability for DNA Stable Isotope Probing (DNA-SIP) of Spores in Soils

Current Microbiology, 2009
To test the suitability of DNA stable isotope probing (DNA-SIP) for characterizing bacterial spore populations in soils, the properties of Bacillus subtilis cells and spores intensely labeled with [(13)C]glucose were characterized. Spore germination, vegetative growth rates, and sporulation efficiency were indistinguishable on glucose versus [(13)C ...
Wayne L, Nicholson   +2 more
openaire   +2 more sources

ออโตโทรฟิคแอมโมเนียออกซิเดชันของแอมโมเนียออกซิไดซิงอาร์เคียและแบคทีเรียในระบบบำบัดน้ำเสียด้วยเทคนิค DNA stable isotope probing (DNA-SIP)

การกำจัดแอมโมเนียในระบบบำบัดน้ำเสียในปัจจุบันเป็นการทำงานร่วมกันระหว่างจุลินทรีย์สองกลุ่ม คือ แอมโมเนียออกซิไดซิงอาร์เคีย (Ammonia-oxidizing archaea, AOA) และแอมโมเนียออกซิไดซิงแบคทีเรีย (Ammonia-Oxidizing bacteria, AOB) จากการศึกษากิจกรรมของจุลินทรีย์กลุ่ม AOB ที่ผ่านมาขณะออกซิไดซ์แอมโมเนียพบว่ามีการทำงานแบบออโตโทรป (Autotroph) คือใช้แหล่งคาร ...
openaire   +1 more source

DNA-Stable Isotope Probing (DNA-SIP) reveals the functional diversity of microbial communities in the brine-seawater interface of the Afifi brine pool

2023
Deep Hypersaline Anoxic Basin (DHAB) are extreme hypersaline environments located on the seafloor which, despite conditions that are hardly favourable to life, can support thriving ecosystems and demonstrate unique microbial communities. Information on the diversity and functioning of microbial communities living in these harsh environments remains ...
openaire   +2 more sources

Biochar accelerated soil atrazine degradation by promoting dechlorination pathway: A novel mechanism revealed by DNA stable isotope probing (DNA-SIP)

Bioresource Technology
Biochar can accelerate atrazine degradation in soils, with surface modification being a widely accepted method to improve the performance. Nevertheless, the underlying mechanisms remain unclear. This study explored the efficiency of modified biochar in facilitating soil atrazine biodegradation with the aid of DNA stable isotope probing (DNA-SIP) and ...
Guiqiong, Yang   +10 more
openaire   +2 more sources

Anaerobic benzene degradation under denitrifying conditions: Peptococcaceae was identified as dominant benzene degrader by Stable Isotope Probing (SIP)

2012
An anaerobic microbial community was enriched in a chemostat that was operated for more than 8 years with benzene and nitrate as electron acceptor. The coexistence of multiple species in the chemostat and the presence of a biofilm, led to the hypothesis that benzene-degrading species coexist in a syntrophic interaction, and that benzene can be degraded
van der Zaan, B.M.   +7 more
openaire   +1 more source

DNA Buoyant Density Shifts During 15N-DNA Stable Isotope Probing

Microbiological Research, 2007
Alison M Cupples
exaly  

Stable Isotope Probing: Technical Considerations When Resolving 15N-Labeled RNA in Gradients

Journal of Microbiological Methods, 2010
Ian R. McDonald, Gareth Lloyd-Jones
exaly  

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