Results 91 to 100 of about 359,491 (304)
Virtual staining of label-free tissue in imaging mass spectrometry
Science AdvancesImaging mass spectrometry (IMS) enables untargeted, highly multiplexed mapping of molecular species in biological tissue with unparalleled chemical specificity and sensitivity. However, most IMS platforms lack microscopy-level spatial resolution and cellular morphological contrast, necessitating subsequent histochemical staining, microscopic imaging ...
Yijie Zhang +8 more
openaire +4 more sources
Pharmacological chromatin remodeling enhances response to estrogen therapy in ER+ breast cancer
Molecular Oncology, EarlyView.Estrogen therapy elicits clinical benefit in ~ 30% of patients with endocrine‐resistant estrogen receptor (ER)‐positive breast cancer. Based on findings that ER transcriptional activation underlies response to estrogen therapy, we tested the effects of epigenetic dysregulation via pharmacological inhibition of histone deacetylases (HDACi).
Anneka L. Johnson Thomas +16 more
wiley +1 more source
, 1993 Staining by uranyl acetate and lead citrate (UA-LC) of immunolabeled sections of unfixed glomerular basement membrane (GBM) digests embedded in low-acid glycol methacrylate (LA-GMA) is poor.
J W Stirling
core +1 more source
PANoptosis in the pathogenesis of myelodysplastic syndromes
Molecular Oncology, EarlyView.PANoptosis, a combination of three types of programmed cell death, is mediated by a large protein complex called a PANoptosome. In healthy bone marrow hematopoietic cells, PANoptosis is restricted by inhibitory signaling. In MDS, bone marrow cells become sensitive to the PANoptotic stimuli due to the aberrant inactivation of inhibitory signaling or ...
Rohit Thalla +4 more
wiley +1 more source
Effect of probenecid on SR101-labeling.
, 2012 A: Overlay of astroglial EGFP-fluorescence and SR101-fluorescence in the VLM after staining in CTRL conditions. B: Overlay of astroglial EGFP- and SR101-fluorescence after staining with Probenecid (Prob, 1 mM). C,D: Statistical comparison shows no change
Christian Schnell (117226) +2 more
core +1 more source
CHIMIA, 2005 Scanning Electrochemical Microscopy (SECM) is used in combination with copper labeling to visualize roteins on surfaces. Proteins are adsorbed on a poly(vinylidene difluoride) (PVDF) membrane and stained using a standard protocol involving ...
Maurizio Carano +2 more
doaj +1 more source
Molecular Oncology, EarlyView.Partial inhibition of focal adhesion kinase (FAK) can paradoxically promote tumor growth, rather than simply producing a weaker antitumor effect than that observed with strong FAK suppression. In breast cancer and melanoma models, targeting p110δ PI3K, particularly in macrophages, counteracted these tumor‐promoting effects, highlighting the importance ...
Lydia Xenou +4 more
wiley +1 more source
Staining for CaBPs within backfilled NM neurons by a NL injection in single optical sections.
, 2013 A, D, G show retrograde labeling. B, E, H show CR, PV and CB positive staining, respectively. C, F, I show double labeling. The arrowheads indicate the double-labeled cells. Scale bar = 20 µm.
Mingxue Zuo (485326) +7 more
core +1 more source
A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining.
PLoS ONE, 2020 Labeling substrates or products are paramount in determining enzymatic kinetic parameters. Several options are available; many laboratories use either radioactive or fluorescent labeling because of their high sensitivity.
Danielle Guillen +4 more
doaj +1 more source
Molecular Oncology, EarlyView.Loss of eIF5A rewires thapsigargin‐mediated endoplasmic reticulum stress responses in multiple myeloma cells by selective translation of mRNA transcripts, consequently dampening thapsigargin‐induced unfolded protein response (UPR) activation and shifting the balance from induction of apoptosis toward cell survival and proliferation.
Demi Both +7 more
wiley +1 more source