Characterizing the zebrafish organizer: microsurgical analysis at the early-shield stage [PDF]
, 1996 The appearance of the embryonic shield, a slight thickening at the leading edge of the blastoderm during the formation of the germ ring, is one of the first signs of dorsoventral polarity in the zebrafish embryo.
Fraser, Scott E., Shih, John
core
CHIMIA, 2005 Scanning Electrochemical Microscopy (SECM) is used in combination with copper labeling to visualize roteins on surfaces. Proteins are adsorbed on a poly(vinylidene difluoride) (PVDF) membrane and stained using a standard protocol involving ...
Maurizio Carano +2 more
doaj +1 more source
Simple, scalable proteomic imaging for high-dimensional profiling of intact systems
Cell, 2015 SUMMARY Combined measurement of diverse molecular and anatomical traits that span multiple levels remains a major challenge in biology. Here, we introduce a simple method that enables proteomic imaging for scalable, integrated, high-dimensional ...
Evan Murray +22 more
semanticscholar +1 more source
A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining.
PLoS ONE, 2020 Labeling substrates or products are paramount in determining enzymatic kinetic parameters. Several options are available; many laboratories use either radioactive or fluorescent labeling because of their high sensitivity.
Danielle Guillen +4 more
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Spectral imaging in preclinical research and clinical pathology. [PDF]
, 2012 Spectral imaging methods are attracting increased interest from researchers and practitioners in basic science, pre-clinical and clinical arenas. A combination of better labeling reagents and better optics creates opportunities to detect and measure ...
Beechem, Joseph +2 more
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Synthesis and Biological Characterization of a New Norbormide Derived Bodipy FL-Conjugated Fluorescent Probe for Cell Imaging [PDF]
, 2018 Background: Norbormide (NRB) is a selective rat toxicant endowed with vasoconstrictor activity confined to the rat peripheral arteries. In a recent work we used a fluorescent derivative of NRB (NRB-AF12), obtained by coupling the NBD fluorophore to the ...
Bova, Sergio +9 more
core +3 more sources
Click histochemistry for whole-mount staining of brain structures
MethodsX, 2019 Labeling of the replicating DNA with synthetic thymidine analogs is commonly used for marking the dividing cells. However, until now this method has only been applied to histological sections.
Alexander A. Lazutkin +2 more
doaj +1 more source
Comparative color stability assessment of orthodontic clear aligners: an in vitro study
Scientific ReportsObjective To evaluate the stain resistance and color stability of four Clear aligners (CA) brands against various beverages at different intervals, along with assessing the elaboration of cleaning agents. Methods In a strictly controlled lab environment,
Nancy Ajwa +3 more
doaj +1 more source
International Journal of Cell Biology, 2016 Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions.
Kazuo Katoh
doaj +1 more source
Cellular distribution of the prion protein in palatine tonsils of mule deer (Odocoileus hemionus) and Rocky Mountain elk (Cervus elaphus nelsoni) [PDF]
, 2019 Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) that affects members of the Cervidae family, including deer (Odocoileus spp.), elk (Cervus Canadensis spp.), and moose (Alces alces spp.).
Belden, E. Lee +2 more
core +1 more source