Results 101 to 110 of about 355,639 (301)

Virtual staining of label-free tissue in imaging mass spectrometry

Science Advances
Imaging mass spectrometry (IMS) enables untargeted, highly multiplexed mapping of molecular species in biological tissue with unparalleled chemical specificity and sensitivity. However, most IMS platforms lack microscopy-level spatial resolution and cellular morphological contrast, necessitating subsequent histochemical staining, microscopic imaging ...
Yijie Zhang, Luzhe Huang, Nir Pillar, Yuzhu Li, Yuhang Li, Lukasz G. Migas, Raf Van de Plas, Jeffrey M. Spraggins, Aydogan Ozcan   +8 more
openaire   +4 more sources

Oncogenic DMTF1β promotes cancer cell motility by regulating autophagy through ULK1 stabilization

Molecular Oncology, EarlyView.
In the current study, we demonstrate that the oncogene DMTF1β regulates ULK1 stability by reducing its proteasomal degradation in cancer cells. This stabilization enables ULK1 to induce autophagy, which in turn facilitates cancer cell migration. Consequently, reduced DMTF1β levels lead to decreased autophagy and impaired cancer cell migration.
Jun Xu, Nicolas J. Niklaus, Anna M. Schläfli, Igor Tokarchuk, Magali Humbert, Federico La Manna, Bich Vu, David Maul, Ramin Radpour, Marianna Kruithof‐de Julio, Inti Zlobec, Jörn Dengjel, Bruce E. Torbett, Mario P. Tschan   +13 more
wiley   +1 more source

Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola, Yasmine Illi, Anna Bircher, Marijn Wilmink, Rachele F. Malagutti, Solenn Ottié, Cordelia Schuler, Pedro A. Ruiz, Cheryl de Vallière, Klaus Seuwen, Gerhard Rogler, Martin Hausmann   +11 more
wiley   +1 more source

Copper Staining/Labeling and Scanning Electrochemical Microscopy Readout of Proteins on Poly(vinylidene difluoride) Membranes

CHIMIA, 2005
Scanning Electrochemical Microscopy (SECM) is used in combination with copper labeling to visualize roteins on surfaces. Proteins are adsorbed on a poly(vinylidene difluoride) (PVDF) membrane and stained using a standard protocol involving ...
Maurizio Carano, Niels Lion, Hubert H. Girault   +2 more
doaj   +1 more source

A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining.

PLoS ONE, 2020
Labeling substrates or products are paramount in determining enzymatic kinetic parameters. Several options are available; many laboratories use either radioactive or fluorescent labeling because of their high sensitivity.
Danielle Guillen, Mika Schievelbein, Kushkumar Patel, Davis Jose, Jonathan Ouellet   +4 more
doaj   +1 more source

PAK1 activation drives divergent resistance mechanisms to aromatase inhibition and tamoxifen in a luminal: A breast cancer model

Molecular Oncology, EarlyView.
Breast cancer remains a major cause of cancer death in women, frequently developing endocrine therapy resistance. This study demonstrates that upregulated p21‐activated kinase 1 (PAK1) activity drives resistance to tamoxifen and long‐term estrogen deprivation in ER+ breast cancer models.
Luisa Schwarzmüller, Janina Müller, Efstathios‐Iason Vlachavas, Lukas Beumers, Lena Fleischhacker, Sara Burmester, Angelika Wörner, Sabine Karolus, Dominic Helm, Cindy Körner, Stefan Wiemann   +10 more
wiley   +1 more source

PVL staining of breast tumoral tissues.

, 2015
A. A Breast tumor TMA (formalin-fixed) was stained and imaged as described in Fig 8A. Tumors were all positive with a vast majority of cancer cells stained. Labeling intensity was estimated on a scale ranging from 1 to 4.
Aymeric Audfray (447681), Carlo Unverzagt (749684), Benoit Busser (749686), Sylvie Lantuejoul (127768), Irene Boos (749683), Amandine Hurbin (749682), Adrien Breiman (749681), Jean-Luc Coll (32116), Mona Beldjoudi (749680), Annabelle Varrot (447685), Mourad Bouras (749685), Jacques Le Pendu (208188), Anne Imberty (75942)   +12 more
core   +1 more source

Spatial and single‐nuclei transcriptomics reveals idiosyncratic and generic patterns in papillary and anaplastic thyroid cancers

Molecular Oncology, EarlyView.
Matched spatial transcriptomics and single‐nuclei RNA‐seq were generated for anaplastic and BRAFV600E papillary thyroid cancers revealing generic and tumor‐specific states occurring in cancer cells and in the tumor microenvironment. In this context, cancer dedifferentiation mirrored organoid maturation through ordered thyroid marker gain/loss ...
Adrien Tourneur, Joel Rodrigues Vitória, Manuel Saiselet, Ligia Craciun, Denis Larsimont, Anne Lefort, Frédérick Libert, Carine Maenhaut, Sabine Costagliola, Maxime Tarabichi, Mirian Romitti, Vincent Detours   +11 more
wiley   +1 more source

EdU and WGA labeling.

, 2013
Transversal section of a tentacle showing histological labeling of EdU+ nuclei (green) and mucocytes (arrow) stained with WGA (red). DAPI staining (blue) was used to visualize nuclei. E, endodermis; G, gastrodermis.
Ove Hoegh-Guldberg (150861), Anne-Catherine Herman (415960), Linda Tonk (415961), Stéphane Roberty (415959), Jean-Christophe Plumier (88146), David Fransolet (415958)   +5 more
core   +1 more source

CEACAM1 participation in breast cancer progression

Molecular Oncology, EarlyView.
In invasive breast cancer (BC), CEACAM1 shifts from an apical to a uniform membranous/cytoplasmic pattern, or is lost, as tumors dedifferentiate, inversely tracking the Ki‐67 proliferative index. In MCF‐7 cells, only CEACAM1‐4L suppresses proliferation, repressing cell cycle and growth factor genes.
Mykola Lyndin, Irina Kube‐Golovin, Anatolii Romaniuk, Gunther Wennemuth   +3 more
wiley   +1 more source