Results 101 to 110 of about 471,495 (309)

Simplified three-dimensional tissue clearing and incorporation of colorimetric phenotyping. [PDF]

, 2016
Tissue clearing methods promise to provide exquisite three-dimensional imaging information; however, there is a need for simplified methods for lower resource settings and for non-fluorescence based phenotyping to enable light microscopic imaging ...
Chen, Harrison, Cheng, Michelle, Di Carlo, Dino, Ding, Yichen, Eguchi, Daniel, Hsiai, Tzung K, Huang, Vincent, Kim, Jocelyn T, Kulkarni, Rajan P, Ma, Jianguo, Nakano, Atsushi, Sung, Kevin, Yang, Cindy F   +12 more
core   +2 more sources

Cell‐cycle‐specific lesion evolution rather than inhibition of double‐strand‐break repair underpins cisplatin radiosensitization

Molecular Oncology, EarlyView.
We analyze cisplatin–DNA adducts (CDAs) and double‐strand breaks (DSBs) in a cell‐cycle‐dependent manner. We find that CDAs form similarly across all cell cycle phases. DSBs arise only in S‐phase. CDAs might not directly impair DSB repair, but S‐phase DSB lesions evolve in the presence of CDAs and disrupt repair in G2, also causing radiosensitization ...
Ye Qiu, Xixi Lin, Emil Mladenov, Veronika Mladenova, Jürgen Thomale, Ali Sak, Yao Wang, Yunxuan Deng, Eleni Gkika, Martin Stuschke, George Iliakis   +10 more
wiley   +1 more source

A Novel Antigen Sensitive to Calcium Chelation That is Associated with the Tip Links and Kinocilial Links of Sensory Hair Bundles [PDF]

, 2003
Tip links are extracellular, cell-surface-associated filaments of unknown molecular composition that are thought to gate the mechanotransducer channel of the sensory hair cell.
Goodyear, Richard J, Richardson, Guy P
core   +2 more sources

NKCC1: A key regulator of glioblastoma progression

Molecular Oncology, EarlyView.
Glioblastoma (GBM) progression is driven by disrupted chloride cotransporter homeostasis. NKCC1 is highly expressed in stem‐like, astrocytic, and progenitor cells, correlating with earlier recurrence, while overall survival remains unaffected. NKCC1 serves as a prognostic marker and potential therapeutic target, linking chloride transporter imbalance ...
Anja Thomsen, Diana Freitag, Madlen Haase, Christian Senft, Falko Schwarz, Silke Keiner   +5 more
wiley   +1 more source

A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining.

PLoS ONE, 2020
Labeling substrates or products are paramount in determining enzymatic kinetic parameters. Several options are available; many laboratories use either radioactive or fluorescent labeling because of their high sensitivity.
Danielle Guillen, Mika Schievelbein, Kushkumar Patel, Davis Jose, Jonathan Ouellet   +4 more
doaj   +1 more source

Increased apoptosis of immunoreactive host cells and augmented donor leukocyte chimerism, not sustained inhibition of B7 molecule expression are associated with prolonged cardiac allograft survival in mice preconditioned with immature donor dendritic cells plus anti-CD40L mAb [PDF]

, 1999
Background. We previously reported the association among donor leukocyte chimerism, apoptosis of presumedly IL-2-deficient graft-infiltrating host cells, and the spontaneous donor-specific tolerance induced by liver but not heart allografts in mice ...
Angus W. Thomson, Aust, Bishop, Bishop, Buhlmann, Cohn, Cuiping Zhong, Dai, Demetris, Durie, Effros, Ehl, Fu, Hancock, Inaba, Janeway, Jenkins, John J. Fung, Kneitz, Konieczny, Larsen, Larsen, Lenschow, Lina Lu, Lu, Lu, Lu, Lu, Lu, Markees, Matzinger, Meyer, Morecki, Murase, Murase, Murase, Niimi, Ohashi, Ohashi, Parker, Picker, Qian, Qian, Rafaeli, Rastellini, Sayegh, Schwartz, Shiguang Qian, Starzl, Starzl, Starzl, Starzl, Sun, Taniguchi, Thomas E. Starzl, Thomson, Thomson, Van Parijs, Wei Li, Wekerle, Willerford, Zinkernagel, Zinkernagel, Zinkernagel   +63 more
core   +1 more source

Fluorogenic cell surface glycan labelling with fluorescence molecular rotor dyes and nucleic acid stains

Chemical Communications
Covalent labelling of sialic acids on live cell surfaces or mucin with fluorescence molecular rotors enables wash-free imaging and provides information about changes occurring to cell surfaces.
Alen Koçak, Amal K. Homer, Antonia Feida, Florian Telschow, Jacob L. Gorenflos López, Cihan Baydaroğlu, Michael Gradzielski, Christian P. R. Hackenberger, Ulrike Alexiev, Oliver Seitz   +9 more
openaire   +2 more sources

Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy

Molecular Oncology, EarlyView.
Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen, Kristiane Søreng, Chiara Veroni, Laura Trachsel‐Moncho, Nagham Asp, Robin Gaupset, Lars Gustav Lyckander, Helene Knævelsrud, Lars Eftang, Anne Simonsen   +9 more
wiley   +1 more source

Mesenchymal adenomatous polyposis coli plays critical and diverse roles in regulating lung development. [PDF]

, 2015
BackgroundAdenomatous polyposis coli (Apc) is a tumor suppressor that inhibits Wnt/Ctnnb1. Mutations of Apc will not only lead to familial adenomatous polyposis with associated epithelial lesions, but will also cause aggressive fibromatosis in ...
Bellusci, Saverio, Chen, Hui, Chiu, Joanne, El Agha, Elie, Luo, Yongfeng, Menke, Douglas B, Qian, Bang-Ping, Shi, Wei, Turcatel, Gianluca, Warburton, David   +9 more
core   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source