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Starch Biosynthesis and Its Manipulation
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Starch biosynthesis in cereal endosperm
Plant Physiology and Biochemistry, 2010Stored starch generally consists of two d-glucose homopolymers, the linear polymer amylose and a highly branched glucan amylopectin that connects linear chains. Amylopectin structurally contributes to the crystalline organization of the starch granule in cereals.
Jong-Seong Jeon +2 more
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Euphytica, 2001
Starch biosynthesis in plants involves the concerted action of a number of enzymes, including ADPglucose pyrophosphorylase, starch synthases, branching enzymes and debranching enzymes. We report on the cloning and characterisation of genes encoding these enzymes from wheat and on their chromosomal locations.
Morell, M. K. +4 more
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Starch biosynthesis in plants involves the concerted action of a number of enzymes, including ADPglucose pyrophosphorylase, starch synthases, branching enzymes and debranching enzymes. We report on the cloning and characterisation of genes encoding these enzymes from wheat and on their chromosomal locations.
Morell, M. K. +4 more
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Carbohydrate Research, 1968
Abstract The inhibition effects of the reagents p-chloromercuribenzoate (pCMB), iodoacetamide (IAA), and N-ethylmaleimide (NEMI) in the reaction catalysed by phosphorylase were investigated. The dissociation constants of the complexes of the various inhibitors formed with the enzyme were established.
J. Holló +3 more
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Abstract The inhibition effects of the reagents p-chloromercuribenzoate (pCMB), iodoacetamide (IAA), and N-ethylmaleimide (NEMI) in the reaction catalysed by phosphorylase were investigated. The dissociation constants of the complexes of the various inhibitors formed with the enzyme were established.
J. Holló +3 more
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Starch biosynthesis: mechanism for the elongation of starch chains
Carbohydrate Research, 2002Starch granules from eight diverse plant sources all had active starch synthases and branching enzymes inside the granules. The enzymes synthesized both amylose and amylopectin from ADPGlc. Pulsing of the granules with ADP-[14C]Glc gave synthesis of starch that on reduction and glucoamylase hydrolysis gave 14C-labeled D-glucitol. The pulsed label could
Rupendra, Mukerjea +2 more
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The Biosynthesis of Starch Granules
Biomacromolecules, 2001Although composed simply of glucose polymers, the starch granule is a complex, semicrystalline structure. Much of this complexity arises from the fact that the two primary enzymes of synthesis-starch synthase and starch-branching enzyme-exist as multiple isoforms.
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Carbohydrate Research, 1969
Abstract The inhibitory effect of d -glucose, 2-deoxy- d - arabino -hexose, 3- O -methyl- d -glucose, and 6- O -methyl- d -glucose on the enzymic synthesis catalysed by potato phosphorylase has been investigated. Kinetic methods showed that, for both of the substrates employed, a competitive inhibitor effect was exerted by each of the substrate ...
J. Holló, E. László, Á. Hoschke
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Abstract The inhibitory effect of d -glucose, 2-deoxy- d - arabino -hexose, 3- O -methyl- d -glucose, and 6- O -methyl- d -glucose on the enzymic synthesis catalysed by potato phosphorylase has been investigated. Kinetic methods showed that, for both of the substrates employed, a competitive inhibitor effect was exerted by each of the substrate ...
J. Holló, E. László, Á. Hoschke
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A phosphorylase involved in starch biosynthesis
Biochemical and Biophysical Research Communications, 1970Abstract A novel phosphorylase, which has no requirement for primer addition, has been isolated from Solanum tuberosum . The properties of this enzyme were found to differ from those of the classical potato phosphorylase. The results are discussed in terms of the possible role of this enzyme in starch biosynthesis.
E, Slabnik, R B, Frydman
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Starch phosphorylation plays an important role in starch biosynthesis
Carbohydrate Polymers, 2017Starch phosphate esters are crucial in starch metabolism and render valuable functionality to starches for various industrial applications. A potato glucan, water dikinase (GWD1) was introduced in tubers of two different potato genetic backgrounds: an amylose-containing line Kardal and the amylose-free mutant amf.
Xu, Xuan +5 more
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Biosynthesis of starch; identification of potato starch enzymes
Food Hydrocolloids, 1987Abstract Two important starch enzymes, granule-bound starch synthase and branching enzyme, were purified from potato tubers and characterized by immunological comparison with the corresponding enzymes of other plants. Granule-bound starch synthase was identified as a 60-kd protein homologous to the corresponding enzymes of maize and amaranth; the ...
G.H. Vos-Scheperkeuter +6 more
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