Results 61 to 70 of about 122 (91)

Precise engineering of gene expression by editing plasticity

Genome Biology
Background Identifying transcriptional cis-regulatory elements (CREs) and understanding their role in gene expression are essential for the precise manipulation of gene expression and associated phenotypes.
Yang Qiu, Lifen Liu, Jiali Yan, Xianglei Xiang, Shouzhe Wang, Yun Luo, Kaixuan Deng, Jieting Xu, Minliang Jin, Xiaoyu Wu, Liwei Cheng, Ying Zhou, Weibo Xie, Hai-Jun Liu, Alisdair R. Fernie, Xuehai Hu, Jianbing Yan   +16 more
doaj   +1 more source

Developmental and housekeeping transcriptional programs display distinct modes of enhancer-enhancer cooperativity in Drosophila

Nature Communications
Genomic enhancers are key transcriptional regulators which, upon the binding of sequence-specific transcription factors, activate their cognate target promoters. Although enhancers have been extensively studied in isolation, a substantial number of genes
Vincent Loubiere, Bernardo P. de Almeida, Michaela Pagani, Alexander Stark   +3 more
doaj   +1 more source

Whole-genome STARR-seq Protocol – Reddy Lab 2023 v1

Reddy lab whole genome STARR-seq protocol. The document includes the input, transfection and output library generation, which should be the expected protocol products. Adapted from the document found in STARR-seq experiment from the Reddy lab in the ENCODE project (document attached with additional details).
Graham Johnson, Kari Strouse, Keith Siklenka, Shauna Morrow, Tim Reddy   +4 more
openaire   +1 more source

Functional characterization of transcriptional enhancers in an Anopheles genetic locus controlling natural resistance to the malaria parasite, Plasmodium falciparum

Epigenetics & Chromatin
Background Anopheles mosquitoes and the malaria parasites they transmit remain a significant global health problem. Most genomic and functional genomic studies of mosquitoes have focused on the protein-coding genome, and comparatively little is known ...
Natalia Marta Zmarlak-Feher, Kathryn S. Taquet, Renée Zakhia, Adrien Pain, Emma Brito-Fravallo, Cameron E. Anderson, Kenneth D. Vernick, Christian Mitri, Michelle M. Riehle   +8 more
doaj   +1 more source

Analysis of biased allelic enhancer activity of schizophrenia-linked common variants

Communications Biology
Schizophrenia is a neuropsychiatric disorder with heritability estimates between 60% and 80%. Although genome-wide association studies have identified many genetic loci linked to the disorder, most of these are noncoding variants whose functional impacts
Chengwen Gao, Qian Zhang, Chuanhong Wu, Lixia Peng, Mingxuan Wu, Baokun Wang, Baiqiang Xue, Mingming Niu, Yajie Jiang, Wenli Yuan, Haiyan Song, Xiangyu Li, Xiangzhong Zhao, Yujuan Niu, Yuanchao Sun, Yonghe Ding, Zhiqiang Li   +16 more
doaj   +1 more source

Abstracts From AIDS 2026, the 26th International AIDS Conference, 26 – 31 July 2026 Rio de Janeiro, Brazil & virtual

Journal of the International AIDS Society, Volume 29, Issue S4, July 2026.
wiley   +1 more source

Validation of Enhancer Regions in Primary Human Neural Progenitor Cells using Capture STARR-seq

Abstract Genome-wide association studies (GWAS) and expression analyses implicate noncoding regulatory regions as harboring risk factors for psychiatric disease, but functional characterization of these regions remains limited. We performed capture STARR-sequencing of over 78,000 candidate regions to identify active enhancers in primary
Sophia C, Gaynor-Gillett, Lijun, Cheng, Manman, Shi, Jason, Liu, Gaoyuan, Wang, Megan, Spector, Mary, Flaherty, Martha, Wall, Ahyeon, Hwang, Mengting, Gu, Zhanlin, Chen, Yuhang, Chen, PsychENCODE, Consortium, Jennifer R, Moran, Jing, Zhang, Donghoon, Lee, Mark, Gerstein, Daniel, Geschwind, Kevin P, White   +18 more
openaire   +2 more sources

HDI-STARR-seq identifies functional GH-regulated sex-biased hepatocyte enhancers linked to liver metabolism and disease

Endocrinology
Abstract Growth hormone (GH) controls sexual dimorphism in hepatocyte gene expression programs governing lipid metabolism, bile acid synthesis and xenobiotic processing, which contribute to sex differences in metabolic dysfunction-associated steatotic liver disease (MASLD) risk. Although GH-regulated sex-specific transcription is well-
Ting-Ya Chang, David J Waxman
openaire   +3 more sources

Enhancer-based Gene Therapy For Digit Regeneration: Identification Of Precision AAV Control Elements With Starr-seq

PURPOSE: Amputations of digits and limbs are common, life-altering injuries, emphasizing the need for therapies that improve healing or even restore lost tissues. Mouse digit tip (MDT) regeneration serves as a unique model of partial limb regrowth, exhibiting comparable anatomic stages to other species capable of complete limb regeneration.
Katja Koll, Alyssa Ehrhardt, Rochelle Bitolas, David Nichols, David Salven, Kendall Reitz, Madelaine Landau, Jianhong Ou, Kenneth Poss, David Brown   +9 more
openaire   +2 more sources

STARR-Seq — Principles and Applications

Genomics, 2015
Differential gene expression is the basis for cell type diversity in multicellular organisms and the driving force of development and differentiation. It is achieved by cell type-specific transcriptional enhancers, which are genomic DNA sequences that activate the transcription of their target genes.
Felix Muerdter, Cosmas D Arnold, Łukasz M Boryń   +2 more
exaly   +3 more sources