Results 11 to 20 of about 25,057 (234)
Intelligent-Illumination STED [PDF]
, 2022 Recently established fluorescence superresolution microscopy techniques, such as stimulated emission depletion (STED), are capable of imaging fixed and living cells at the nanometer scale. In STED, ON- and OFF-switching of fluorophores is performed with an excitation and a STED laser beam, respectively.
Heine, Jörn +4 more
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, 2022 Die räumliche Auflösung optischer Fernfeldmikroskope war durch Beugung begrenzt, bis STED-Mikroskopie das Beugungslimit durchbrach. Diese Doktorarbeit kombiniert zum ersten Mal die hohe räumliche Auflösung dieser Technik mit hoher zeitlicher Auflösung zur Schnellen (strahlabtastenden) STED-Mikroskopie.
Lauterbach, Marcel +3 more
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STED super-resolved microscopy
Nature Methods, 2018 Stimulated emission depletion (STED) microscopy provides subdiffraction resolution while preserving useful aspects of fluorescence microscopy, such as optical sectioning, and molecular specificity and sensitivity. However, sophisticated microscopy architectures and high illumination intensities have limited STED microscopy's widespread use in the past.
Giuseppe Vicidomini +2 more
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Protected STED and multicolour multilevel STED nanoscopy.
, 2016 The diffraction barrier of focused light has been overcome by modern superresolution fluorescence microscopy techniques. These methods harness transitions between signaling and non-signaling states of the marker molecules to make them discernible at length scales of few tens of nanometers. Although superresolution methods like STED (STimulated Emission
Sidenstein, Sven-Christian +1 more
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STED Microscopy of FRET Pairs [PDF]
, 2022 Der Förster-Resonanzenergietransfer (FRET) ist ein beliebtes Werkzeug der Lebenswissenschaften. Er wird z.B. benutzt um Proteininteraktionen oder das Binden von Liganden zu detektieren, oder um fluoreszente Biosensoren für Stoffwechselprodukte oder Ionen zu konstruieren.
Loidolt-Krüger, Maria +4 more
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, 2010 This work discusses rugged technical simplifications for STED nanoscopy and methods related to it (RESOLFT). STED is a type of laser scanning microscopy that uses stimulated emission in order to inhibit spontaneous fluorescence from outer parts of the focal spot.
Reuss, Matthias
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STED microscopy in the visible range [PDF]
, 2006 Light microscopy is a key scientific instrument in the life sciences. However, the resolution of far-field light microscopy is limited by diffraction. Exploiting a saturated depletion of the molecular excited state by stimulated emission, stimulated emission depletion (STED) breaks the resolution barrier in the important subfield of fluorescence ...
Willig, Katrin I., Willig, K.
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Foundations of Sted Microscopy
, 2014 This chapter presents the foundations of STED microscopy with a comparison to its generalization Resolft microscopy and to stochastic imaging methods (PALM, STORM, FPALM, and alike). The first section reviews the advantages of optical microscopy, explains the diffraction limit, and shows how the classical resolution limit was finally broken.
Lauterbach, M, Eggeling, C
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A STED microscope aligned by design
Optics Express, 2009 STED microscopes are commonly built using separate optical paths for the excitation and the STED beam. As a result, the beams must be co-aligned and can be subject to mechanical drift. Here, we present a single-path STED microscope whose beams are aligned by design and hence is insensitive to mechanical drift.
Wildanger, Dominik +4 more
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Parallelized STED fluorescence nanoscopy
Optics Express, 2011 We introduce a parallelized STED microscope featuring m = 4 pairs of scanning excitation and STED beams, providing m-fold increased imaging speed of a given sample area, while maintaining basically all of the advantages of single beam scanning. Requiring only a single laser source and fiber input, the setup is inherently aligned both spatially and ...
Bingen, Pit +3 more
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