Results 31 to 40 of about 25,057 (234)

STED Nanoscopy [PDF]

open access: yes, 2020
AbstractThis chapter provides an introduction to the fundamentals of STED nanoscopy. The first section starts with a discussion of the field and intensity distribution in the vicinity of the geometric focus by means of vectorial diffraction theory.
Egner, Alexander   +5 more
openaire   +2 more sources

Barn og unge: By, sted og sosio materialitet av Sissel Seim og Oddrun Sæter (red.) [PDF]

open access: yes, 2018
Synopsis Hva er barn og unges vilkår i byen? Hvilke betingelser gir byen for samspillet mellom mennesker og byens rom og steder? Hvilke sosiomaterielle vilkår for deltakelse finnes i institusjoner for barn og unge? Kan byrom, steder og sosiomaterialitet
Seim, Sissel   +2 more
core   +1 more source

Hjemme på stølen

open access: yesNorsk Antropologisk Tidsskrift, 2020
Sammendrag Hjem analyseres som et relasjonelt og dynamisk sted. Budeiene artikkelen handler om, lager stølen til sitt hjem, samtidig som landskap, materialitet og praksiser påvirker dem.
Anne-Katrine Brun Norbye
doaj   +1 more source

Event-triggered STED imaging

open access: yesNature Methods, 2022
Abstract Monitoring the proteins and lipids that mediate all cellular processes requires imaging methods with increased spatial and temporal resolution. STED (stimulated emission depletion) nanoscopy enables fast imaging of nanoscale structures in living cells but is limited by photobleaching. Here, we present event-triggered STED, an
Jonatan Alvelid   +3 more
openaire   +3 more sources

The Salmonella transmembrane effector SteD hijacks AP1-mediated vesicular trafficking for delivery to antigen-loading MHCII compartments.

open access: yesPLoS Pathogens, 2022
SteD is a transmembrane effector of the Salmonella SPI-2 type III secretion system that inhibits T cell activation by reducing the amounts of at least three proteins -major histocompatibility complex II (MHCII), CD86 and CD97 -from the surface of antigen-
Camilla Godlee   +6 more
doaj   +1 more source

New Concepts for STED Microscopy [PDF]

open access: yes, 2021
Fluorescence nanoscopy allows to non-invasively resolve three-dimensional cellular structures beyond the diffraction limit. One of these high resolution imaging techniques is stimulated emission depletion (STED) microscopy.
Kratz, Julia
core   +1 more source

Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator. [PDF]

open access: yesPLoS ONE, 2012
In this report, a Ti:Sapphire oscillator was utilized to realize synchronization-free stimulated emission depletion (STED) microscopy. With pump power of 4.6 W and sample irradiance of 310 mW, we achieved super-resolution as high as 71 nm.
Yujia Liu   +9 more
doaj   +1 more source

STED nanoscopy with time-gated detection: theoretical and experimental aspects.

open access: yesPLoS ONE, 2013
In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event.
Giuseppe Vicidomini   +6 more
doaj   +1 more source

Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell Imaging

open access: yesBiosensors, 2021
Stimulated emission depletion (STED) microscopy is a typical laser-scanning super-resolution imaging technology, the emergence of which has opened a new research window for studying the dynamic processes of live biological samples on a nanometer scale ...
Jia Zhang   +7 more
doaj   +1 more source

mfRFP performance on STED micrscopy

open access: yes, 2022
   Figure 3. Confocal imaging with selected FPs. (a) Time-lapse confocal (top row) and STED (bottom row) imaging of representative cells expressing H2B-mfRFP (n=4 and 5 cells from 2 independent transfections for confocal and STED imaging, respectively ...
Olumayowa Fakorede (14073456)
core   +1 more source

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