Results 21 to 30 of about 25,057 (234)
A straightforward STED-background corrected fitting model for unbiased STED-FCS analyses
Methods, 2018 Combining stimulated emission depletion and fluorescence correlation spectroscopy (STED-FCS) provides a powerful and sensitive tool for studying the molecular dynamics in live cells with high spatio-temporal resolution. STED-FCS gives access to molecular diffusion characteristic at the nanoscale occurring within short period of times.
Wang, Ruixing +7 more
openaire +4 more sources
Vortex fibers for STED microscopy
APL Photonics, 2019 Super-resolution imaging using the principles of stimulated emission depletion (STED) microscopy requires collinear excitation of a sample with a Gaussian-shaped excitation beam and a donut-shaped depletion beam whose spin (polarization) and orbital ...
Lu Yan +2 more
doaj +2 more sources
A Molecular Engineering Strategy to Fine-Tune Phototoxicity of AIE Probes for Super-Resolution Imaging of Mitochondrial Cristae Dynamics. [PDF]
Adv Sci (Weinh)Regulation of molecular packing, phototoxicity, and ROS output of AIE photosensitizers is achieved through carboxylation and alkyl‐chain‐length tuning. The probe designed via this strategy enables super‐resolution visualization of inner‐mitochondrial‐membrane cristae dynamics under controlled oxidative stress, and fluorescence‐lifetime imaging of ...
Chen K +5 more
europepmc +2 more sources
STED-Inspired Cationic Photoinhibition Lithography
, 2023 Direct laser writing by two-photon lithography has been enhanced substantially during the past two decades by techniques borrowed from stimulated emission depletion (STED) microscopy.
Thomas A. Klar +5 more
core +1 more source
Journal of Innovative Optical Health Sciences, 2021 Clathrin- and caveolae-mediated endocytosis are the most commonly used pathways for the internalization of cell membrane receptors. However, due to their dimensions are within the diffraction limit, traditional fluorescence microscopy cannot distinguish ...
Hefei Ruan +5 more
doaj +1 more source
Barns fiskevær: Jenters og gutters uteaktiviteter som bidrag til konstruksjon av sted og kjønn
Barn, 2022 Artikkelen handler om barn og deres utendørsaktiviteter i et nordnorsk fiskevær som jeg velger å kalle for Fiskeværet. Barna, dvs. fire jenter og fem gutter i åtte-tiårsalderen, gikk på en skole der elever fra flere trinn var samlet i en og samme klasse,
Siri Gerrard
doaj +1 more source
Fluorescence correlation spectroscopy with a total internal reflection fluorescence STED microscope (TIRF-STED-FCS). [PDF]
, 2012 We characterize a novel fluorescence microscope which combines the high spatial discrimination of a total internal reflection epi-fluorescence (epi-TIRF) microscope with that of stimulated emission depletion (STED) nanoscopy.
Eggeling, C. +14 more
core +2 more sources
Nanophotonics, 2020 Stimulated emission depletion (STED) microscopy breaks the optical diffraction barrier and has become a powerful tool for biological study. However, its application for in vivo study is limited because of its high demand for depletion power.
Wang Luwei +8 more
doaj +1 more source
PLoS Pathogens, 2021 The Salmonella enterica effector SteD depletes mature MHC class II (mMHCII) molecules from the surface of infected antigen-presenting cells through ubiquitination of the cytoplasmic tail of the mMHCII β chain.
Ondrej Cerny +8 more
doaj +1 more source
Biomedical Optics Express, 2020 Stimulated emission depletion (STED) microscopy is a versatile imaging method with diffraction-unlimited resolution. Here, we present a novel STED microscopy variant that achieves either increased resolution at equal laser power or identical super-resolution conditions at significantly lower laser power when compared to the classical implementation. By
Jennifer-Rose Krüger +3 more
openaire +4 more sources