Results 51 to 60 of about 25,057 (234)
STED nanoscopy of the living brain [PDF]
, 2022 Over the past decade, a new class of fluorescence microscopes has evolved which thoroughly break the diffraction limit of classical far-field light microscopy and thus provide significantly improved spatial resolution. Especially so-called STED microscopes have shown to provide valuable information otherwise inaccessible when investigating molecular ...
Berning, Sebastian +5 more
openaire +3 more sources
Deep learning enables fast, gentle STED microscopy
Communications Biology, 2023 STED microscopy is widely used to image subcellular structures with super-resolution. Here, we report that restoring STED images with deep learning can mitigate photobleaching and photodamage by reducing the pixel dwell time by one or two orders of ...
Vahid Ebrahimi +6 more
doaj +1 more source
Adaptive-illumination STED nanoscopy [PDF]
Proceedings of the National Academy of Sciences, 2017 Significance We demonstrate DyMIN (Dynamic Intensity Minimum), a versatile illumination concept for coordinate-targeted superresolution fluorescence imaging. Dynamically adapting the illumination (and therefore resolution) for the probing of molecular signals to local structural features entails major reductions in the light dose ...
Jörn Heine +5 more
openaire +5 more sources
Relevans gjennom kroppslig erfaring og refleksiv viten?
Norsk MuseumstidsskriftHvordan kan handelsstedet Kvitnesgården i Perspektivet Folkeparken være relevant for unge mennesker gjennom at de blir invitert til medskaping av en utstilling?
Marianne A. Olsen
doaj +1 more source
Engineering Microbial Particles for Next‐Generation Biomedical Platforms
Advanced Science, EarlyView.Microbe‐derived particles (MDPs), which include extracellular vesicles, outer membrane vesicles, inclusion bodies, polysaccharide particles, and virus‐like particles, represent a rapidly expanding category of bioinspired nanomaterials. With their natural origin, intrinsic biocompatibility, and highly programmable functionality, MDPs serve as a ...
Yuting Li +7 more
wiley +1 more source
Barnehagegarderoben som dynamisk flerspråklig sted
MålbrytingStudien undersøker hvordan barn, foreldre og ansatte i en barnehage skaper et sosialt rom i barnehagens garderobe gjennom (språklige) handlinger og språkvalg.
Florian Hiss, Anja Maria Pesch
doaj +1 more source
Advanced Science, EarlyView.The promoted Escherichia coli‐assisted continuous evolution (PEACE) system operates through a stringent three step genetic circuit: target gene mutation via a deaminase–T7 RNA polymerase (T7 RNAP) fusion, coupling variant function to antitoxin expression, and growth based selection of surviving cells.
Xinyu Zhang +10 more
wiley +1 more source
Background reduction in STED-FCS using coherent-hybrid STED [PDF]
, 2020 Abstract Fluorescence correlation spectroscopy (FCS) is a valuable tool to study the molecular dynamics of living cells. When used together with a super-resolution stimulated emission depletion (STED) microscope, STED-FCS can measure diffusion processes at the nanoscale in living cells.
Barbotin, Aurélien +4 more
openaire +1 more source
Massively parallelized STED nanoscopy [PDF]
, 2016 Fluorescence microscopy constitutes a key method for the virtually non-invasive study of biological structures and processes on a subcellular scale. Stimulated Emission Depletion (STED) nanoscopy extends fluorescence imaging to nanometer resolutions ...
Bergermann, Fabian Manuel
core +1 more source