Results 61 to 70 of about 558 (261)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

A Core-sensitive Mixed f/g-mode of the Sun Predicted by Wave Topology and Hydrodynamical Simulation

open access: yesThe Astrophysical Journal Letters
Helioseismology has revolutionized our understanding of the Sun by analyzing its global oscillation modes. However, the solar core remains elusive, limiting a full understanding of its evolution.
Arthur Le Saux   +4 more
doaj   +1 more source

Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model

open access: yesFEBS Open Bio, EarlyView.
This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito   +7 more
wiley   +1 more source

Using magnetic fluids to simulate convection in a central force field in the laboratory [PDF]

open access: yesNonlinear Processes in Geophysics, 2005
Large-scale convection in planetary or stellar interiors plays a significant role but it is difficult to reproduce the central force field of those systems in experimental studies.
W.-G. Früh
doaj  

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Zones of Tayler Instability in Stars

open access: yesThe Astrophysical Journal
The Tayler instability (TI) of toroidal magnetic fields is a candidate mechanism for driving turbulence, angular momentum (AM) transport, and dynamo action in stellar radiative zones. Recently V. A. Skoutnev & A. M.
Valentin A. Skoutnev   +1 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

Dimethyl Fumarate, But Not Rituximab, Reduces Serum GFAP Levels and PIRMA in Relapsing–Remitting MS

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Serum neurofilament light chain (sNfL) and glial fibrillary acidic protein (sGFAP) levels are believed to reflect mainly acute and chronic disease processes in multiple sclerosis (MS), respectively. In this study, we investigated whether dimethyl fumarate (DMF) and rituximab (RTX) differentially affect these biomarkers.
F. Shawket   +14 more
wiley   +1 more source

Evolution of a Long-lived Deep-seated Main-sequence Magnetic Field during White Dwarf Cooling

open access: yesThe Astrophysical Journal
We study the evolution of white dwarf (WD) magnetic fields that originate from convective-core dynamos during the main sequence. Using stellar evolution and WD cooling models combined with magnetic field diffusion calculations, we demonstrate that a ...
Matias Castro-Tapia   +2 more
doaj   +1 more source

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