Results 91 to 100 of about 7,802 (179)

The expanding toolkit of translating ribosome affinity purification [PDF]

, 2017
Translating ribosome affinity purification is a method initially developed for profiling mRNA from genetically defined cell types in complex tissues. It has been applied both to identify target molecules in cell types that are important for controlling a
Dougherty, Joseph D
core   +2 more sources

Bioengineered AAV9 and Optimised Microdystrophin Vectors Augment Phenotypic Rescue in a Murine Model of Duchenne Muscular Dystrophy

Journal of Cellular and Molecular Medicine, Volume 30, Issue 5, March 2026.
ABSTRACT Duchenne muscular dystrophy (DMD) is a severe neuromuscular disorder without an effective cure. Adeno‐associated virus (AAV) based gene therapy has improved dystrophin function, with sub‐optimal clinical outcomes. We reasoned that a combination of rational engineering of AAV9 capsids modified at the post‐translational modification sites ...
Mohankumar B. Senthilkumar, Sanya Sharma, Navaneeth Srinivasan, Anila Varghese, Pratiksha Sarangi, Vijayata Singh, Narendra Kumar, Devyani Yenurkar, Sudip Mukherjee, Sonal Amit, Sameer Bhatia, Santosh K. Misra, Ratna Dua Puri, Jeffrey Chamberlain, Giridhara R. Jayandharan   +14 more
wiley   +1 more source

The mRNA architecture of the translation termination site primes programmed stop codon readthrough events in Drosophila

RNA Biology
Programmed stop codon readthrough (SCR) is a form of genetic re-coding, in which a near-cognate tRNA base-pairs with a stop codon, leading to the translation of a C-terminally extended protein.
Laveena Kansara, Georg Wolfstetter, Daniel Wintermayr, Ioannis Alexopoulos, Alejandra Escos, Marc Friedländer, Ylva Engström   +6 more
doaj   +1 more source

Non-canonical Translation in Plant RNA Viruses [PDF]

, 2017
Viral protein synthesis is completely dependent upon the host cell's translational machinery. Canonical translation of host mRNAs depends on structural elements such as the 5′ cap structure and/or the 3′ poly(A) tail of the mRNAs.
Manuel Miras, Miguel A. Aranda, Verónica Truniger, W. Allen Miller   +3 more
core   +1 more source

STIMATE‐Mustn1 Fusion Protein Prevents Ca2+ Overload in Cells That Present Increased SOCE

The FASEB Journal, Volume 40, Issue 4, 28 February 2026.
STIMATE‐mustn1 fused protein expression downregulates Thr‐evoked Ca2+ entry in neonatal platelets. Meanwhile, maternal platelets express the canonical STIMATE, which promotes the Thr‐dependent Ca2+ entry. Impaired Ca2+ entry in neonatal platelets may contribute to the alteration in platelet aggregation found in these subjects. ER, endoplasmic reticulum.
Alejandro Berna‐Erro, Pedro J. Camello, María P. Granados, Elena Delgado, Esperanza Fernández, Raúl Teruel‐Montoya, Francisca Ferrer‐Marín, Juan A. Rosado, Pedro C. Redondo   +8 more
wiley   +1 more source

Identification and functional analysis of novel phosphorylation sites in the RNA surveillance protein Upf1. [PDF]

, 2013
One third of inherited genetic diseases are caused by mRNAs harboring premature termination codons as a result of nonsense mutations. These aberrant mRNAs are degraded by the Nonsense-Mediated mRNA Decay (NMD) pathway.
Bracho, Dina P, Cajigas, Iván J, Correa, María E, Estrella, Luis A, González, Carlos I, González-Feliciano, José A, Lasalde, Clarivel, León, Alfredo J, Rivera, Andrea V, Rodríguez-Cruz, Eva N, Vega, Irving E, Wilkinson, Miles F   +11 more
core   +1 more source

Differential regulation of translational stress responses by herpesvirus ubiquitin deconjugases

The FEBS Journal, Volume 293, Issue 4, Page 1024-1044, February 2026.
Translating viral mRNAs is challenging due to structural features that may slow translation or induce ribosome stalling. The viral ubiquitin deconjugases encoded by human pathogenic herpesviruses regulate the cellular response to ribosomal stress by inhibiting various branches of the Ribosomal Quality Control (RQC) and activating Ribosomal Stress ...
Jiangnan Liu, Noemi Nagy, Carlos Mario Ayala‐Torres, Maria G. Masucci   +3 more
wiley   +1 more source

Mining prokaryotic genomes for unknown amino acids: a stop-codon-based approach

BMC Bioinformatics, 2007
Background Selenocysteine and pyrrolysine are the 21st and 22nd amino acids, which are genetically encoded by stop codons. Since a number of microbial genomes have been completely sequenced to date, it is tempting to ask whether the 23rd amino acid is ...
Esaki Nobuyoshi, Goto Susumu, Mihara Hisaaki, Fujita Masashi, Kanehisa Minoru   +4 more
doaj   +1 more source

A rhlI 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in Pseudomonas aeruginosa [PDF]

, 2019
N-Acyl homoserine lactone (AHL) quorum sensing (QS) controls expression of over 200 genes in Pseudomonas aeruginosa. There are two AHL regulatory systems: the LasR-LasI circuit and the RhlR-RhlI system.
Addis, Victoria, Dar, Daniel, Fitzgerald, David, Gottesman, Susan, Greenberg, E. Peter, Sorek, Rotem, Thomason, Maureen K., Voichek, Maya   +7 more
core   +1 more source

Genetic Encoding of Pentafluorophosphato‐Phenylalanine Provides PF5‐Proteins as Phosphoprotein Mimetics

Angewandte Chemie International Edition, Volume 65, Issue 1, 2 January 2026.
Genetic code expansion with a unique hyper‐fluorinated phosphotyrosine analog. In this work, we successfully incorporated the unnatural amino acid pentafluorophosphato‐difluoromethyl‐phenylalanine, carrying seven fluorine atoms and a permanent negative charge into three different proteins via the use of mutated orthogonal aminoacyl‐tRNA synthetases ...
Anna Magdalena Ambros, Haocheng Qianzhu, Markus Tiemann, Edan Habel, Katrin Denzinger, Hana Zupan, Matteo Accorsi, Bettina G. Keller, Gerhard Wolber, Thomas Huber, Jörg Rademann   +10 more
wiley   +1 more source