Results 111 to 120 of about 7,802 (179)

Investigating stop codon readthrough in Saccharomyces cerevisiae

, 2021
Stop codon readthrough occurs via genetic and epigenetic mechanisms, resulting in a longer polypeptide chain at the C-terminus. Although these readthroughs may seem like an error made by translational mechanisms, evidence from yeast suggests that stop codon readthrough has an impact on various cellular processes. Readthrough has the potential to create
openaire   +1 more source

The Effect of Mutation at Thr 295 of Saccharomyces cerevisiae eRF1 on Suppression of Nonsense Codons and eRF1 Structure

Microbiology Indonesia, 2008
The termination of translation in Saccharomyces cerevisiae is controlled by two interacting polypeptide chain release factors, eRF1, and eRF3. Two regions in eRF1, at position 281-305 and 411-415, were proposed to be involved on the interaction to eRF3 ...
PRIMA ENDANG SUSILOWATI, PINGKAN ADITIAWATI, FIDA MADAYANTI, . AKHMALOKA   +3 more
doaj   +1 more source

The alphaviruses: gene expression, replication, and evolution [PDF]

, 1994
The alphaviruses are a genus of 26 enveloped viruses that cause disease in humans and domestic animals. Mosquitoes or other hematophagous arthropods serve as vectors for these viruses.
Strauss, Ellen G., Strauss, James H.
core   +1 more source

Advances in therapeutic use of a drug-stimulated translational readthrough of premature termination codons

Molecular Medicine, 2018
Premature termination codons (PTCs) in the coding regions of mRNA lead to the incorrect termination of translation and generation of non-functional, truncated proteins.
Maciej Dabrowski, Zuzanna Bukowy-Bieryllo, Ewa Zietkiewicz   +2 more
doaj   +1 more source

Stop-Codon Readthrough in Therapeutic Protein Candidates Expressed from Mammalian Cells

Journal of Pharmaceutical Sciences
Stop codon readthroughs were examined in 48 recombinant therapeutic protein candidates produced from multiple clones of Chinese hamster ovary cells, using peptide mapping with LC-MS/MS detection. We found that stop codon readthrough is a common phenomenon occurring in most of these candidates, with levels varying from below the detection limit of ∼0 ...
Zhongqi Zhang, Neelam Khanal, Andrew B. Dykstra, Kristi Daris   +3 more
openaire   +2 more sources

A proposed new Tombusviridae genus featuring extremely long 5’ untranslated regions and a luteo/polerovirus-like gene block

Frontiers in Virology
Tombusviridae is a large family of single-stranded, positive-sense RNA plant viruses with uncapped, non-polyadenylated genomes encoding 4–7 open reading frames (ORFs).
Zachary Lozier, Zachary Lozier, Lilyahna Hill, Elizabeth Semmann, W. Allen Miller, W. Allen Miller   +5 more
doaj   +1 more source

Fluorescent reporters give new insights into antibiotics-induced nonsense and frameshift mistranslation

Scientific Reports
We developed a reporter system based on simultaneous expression of two fluorescent proteins: GFP as a reporter of the capacity of protein synthesis and mutated mScarlet-I as a reporter of translational errors.
Mariliis Hinnu, Marta Putrinš, Karin Kogermann, Niilo Kaldalu, Tanel Tenson   +4 more
doaj   +1 more source

Translation velocity determines the efficacy of engineered suppressor tRNAs on pathogenic nonsense mutations

Nature Communications
Nonsense mutations – the underlying cause of approximately 11% of all genetic diseases – prematurely terminate protein synthesis by mutating a sense codon to a premature stop or termination codon (PTC).
Nikhil Bharti, Leonardo Santos, Marcos Davyt, Stine Behrmann, Marie Eichholtz, Alejandro Jimenez-Sanchez, Jeong S. Hong, Andras Rab, Eric J. Sorscher, Suki Albers, Zoya Ignatova   +10 more
doaj   +1 more source

Recent advances in gene therapy for thalassemia

Journal of Pharmacy and Bioallied Sciences, 2012
Thalassemias are genetically transmitted disorders. Depending upon whether the genetic defects or deletion lies in transmission of α or β globin chain gene, thalassemias are classified into α and β-thalassemias.
J V Raja, M A Rachchh, R H Gokani
doaj   +1 more source

Discovery of functional elements in 12 Drosophila genomes using evolutionary signatures [PDF]

, 2007
Sequencing of multiple related species followed by comparative genomics analysis constitutes a powerful approach for the systematic understanding of any genome.
Pachter, Lior, Stark, Alexander
core   +1 more source