, 1998 A convenient test system was designed to investigate the efficiencies of selenocysteine inserting sequences (SECIS) responsible for the cotranslational incorporation of selenocysteine into selenoproteins of mammals.
McCarthy, J. E G +3 more
core +10 more sources
Análisis genético y molecular del síndrome de Maroteaux-Lamy [PDF]
, 2008 [spa] Esta tesis es una contribución al conocimiento del síndrome de Maroteux-Lamy en el terreno de la genética molecular. El síndrome de Maroteaux-Lamy o mucopolisacaridosis de tipo VI (MPS VI) es una grave enfermedad hereditaria muy poco frecuente en ...
Garrido Fernández, Elena
core +6 more sources
Genetic dissection of human ABCE1 in yeast reveals separable requirements for ribosome recycling and suppression of aberrant reinitiation. [PDF]
FEBS Open BioHuman ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Nakata E, Li Y, Endo K, Ito K.
europepmc +2 more sources
Expanding the chemical diversity of M13 bacteriophage
Frontiers in Microbiology, 2022 Bacteriophage M13 virions are very stable nanoparticles that can be modified by chemical and genetic methods. The capsid proteins can be functionalized in a variety of chemical reactions without loss of particle integrity.
Grace L. Allen +6 more
doaj +1 more source
Alteration in H-bond strength affects the stability of codon-anticodon interaction at in-frame UAG stop codon during in vitro translation [PDF]
, 2021 We have studied the decoding ability of a non-standard nucleobase modified tRNA for non-natural amino acid mutagenesis. The insertion of 2, 6-diaminopurine (D) base at the 3rd position of a tRNA anticodon enabled us to evaluate the effect of an ...
Ishu, Saraogi, Purnima , Mala
core +1 more source
Cell Reports, 2023 Summary: Suppression of premature termination codons (PTCs) by translational readthrough is a promising strategy to treat a wide variety of severe genetic diseases caused by nonsense mutations. Here, we present two potent readthrough promoters—NVS1.1 and
Lukas-Adrian Gurzeler +18 more
doaj +1 more source
Frontiers in Chemistry, 2022 The expansion of the genetic code beyond a single type of noncanonical amino acid (ncAA) is hindered by inefficient machinery for reassigning the meaning of sense codons.
Wil Biddle +3 more
doaj +1 more source
Enhanced yield of recombinant proteins with site-specifically incorporated unnatural amino acids using a cell-free expression system. [PDF]
PLoS ONE, 2013 Using a commercial protein expression system, we sought the crucial elements and conditions for the expression of proteins with genetically encoded unnatural amino acids.
Sviatlana Smolskaya +2 more
doaj +1 more source
Suppression of amber codons in Caulobacter crescentus by the orthogonal Escherichia coli histidyl-tRNA synthetase/tRNAHis pair. [PDF]
PLoS ONE, 2013 While translational read-through of stop codons by suppressor tRNAs is common in many bacteria, archaea and eukaryotes, this phenomenon has not yet been observed in the α-proteobacterium Caulobacter crescentus.
Jae-hyeong Ko +4 more
doaj +1 more source
Translation initiation factor eIF3 promotes programmed stop codon readthrough. [PDF]
, 2015 Programmed stop codon readthrough is a post-transcription regulatory mechanism specifically increasing proteome diversity by creating a pool of C-terminally extended proteins.
von der Haar, Tobias +4 more
core +1 more source