Results 91 to 100 of about 326,708 (370)
Molecular Oncology, EarlyView.CircCCNB1 expression is down‐regulated in nasopharyngeal carcinoma (NPC); thus, less NF90 protein is bound to circCCNB1 and more binds to pri‐miRNAs, blocking their (pri‐miRNAs) binding to DGCR8 and inhibiting the processing and generation of miR‐15b‐5p/miR‐7‐1‐3p. Furthermore, decreased miR‐15b‐5p/miR‐7‐1‐3p promotes the expression of the target genes
Chunmei Fan +6 more
wiley +1 more source
BioTechniques, 2013 The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson +2 more
doaj +1 more source
Mirror-image streptavidin with specific binding to L-biotin, the unnatural enantiomer
Scientific Reports, 2022 The streptavidin–biotin system is known to have a very high affinity and specificity and is widely used in biochemical immunoassays and diagnostics. However, this method is affected by endogenous D-biotin in serum sample measurements (biotin interference)
Masatoshi Suganuma +9 more
doaj +1 more source
Comparison of Avidin, Neutravidin, and Streptavidin as Nanocarriers for Efficient siRNA Delivery.
Molecular Pharmaceutics, 2017 Protein-based drug delivery carrier has been one of the most employed modalities in biopharmaceuticals. In this study, we have compared avidin and its two analogues, neutravidin and streptavidin, as nanocarriers for the delivery of biotin-labeled siRNA ...
Akshay Jain +4 more
semanticscholar +1 more source
Molecular Oncology, EarlyView.B‐cell chronic lymphocytic leukemia (B‐CLL) and monoclonal B‐cell lymphocytosis (MBL) show altered proteomes and phosphoproteomes, analyzed using mass spectrometry, protein microarrays, and western blotting. Identifying 2970 proteins and 316 phosphoproteins, including 55 novel phosphopeptides, we reveal BCR and NF‐kβ/STAT3 signaling in disease ...
Paula Díez +17 more
wiley +1 more source
Protein Array Fabrication with E-jet Printing [PDF]
, 2012 We demonstrate the feasibility of fabricate biosensors with direct protein printing without complicated processes at high resolution and area coverage. Printed single protein, streptavidin-fluorescein, binds to biotin labeled with fluorescent DNA without
Rogers, John A.
core
Dynamic removal of replication protein A by Dna2 facilitates primer cleavage during Okazaki fragment processing in Saccharomyces cerevisiae [PDF]
, 2008 Eukaryotic Okazaki fragments are initiated by an RNA/DNA primer, which is removed before the fragments are joined. Polymerase d displaces the primer into a flap for processing.
Bambara, Robert A. +3 more
core +3 more sources
Structural studies of the streptavidin binding loop [PDF]
Protein Science, 1997 AbstractThe streptavidin‐biotin complex provides the basis for many important biotechnological applications and is an interesting model system for studying high‐affinity protein‐ligand interactions. We report here crystallographic studies elucidating the conformation of the flexible binding loop of streptavidin (residues 45 to 52) in the unbound and ...
Ronald E. Stenkamp +4 more
openaire +3 more sources
Loss of proton‐sensing GPR4 reduces tumor progression in mouse models of colon cancer
Molecular Oncology, EarlyView.G protein‐coupled receptor 4 (GPR4) is a pH‐sensing receptor activated by acidic pH. GPR4 expression is increased in patients with inflammatory bowel disease who are at high risk of developing colorectal cancer. In mouse models, loss of GPR4 attenuated tumor progression. This correlated with increased IL2 and natural killer cell activity.
Leonie Perren +16 more
wiley +1 more source
Frontiers in Molecular Biosciences, 2022 A rationally designed gold-functionalized surface capable of capturing a target protein is presented using the biotin–streptavidin pair as a proof-of-concept. We carried out multiscale simulations to shed light on the binding mechanism of streptavidin on
Sutapa Dutta +8 more
doaj +1 more source