Results 91 to 100 of about 318,069 (262)

A high throughput molecular force assay for protein-DNA interactions. [PDF]

, 2011
An accurate and genome-wide characterization of protein–DNA interactions such as transcription factor binding is of utmost importance for modern biology. Powerful screening methods emerged.
Albrecht, Albrecht, Bartels, Berger, Bizzarri, Blank, Bulyk, Bustamante, Bustamante, Cao, Dominik Ho, Dose, Evans, Gittes, Harbison, Hermann E. Gaub, Ho, Ho, Iyer, Jeltsch, Jen-Jacobson, Koch, Koch, Kuhner, Lee, Lesser, Maerkl, Merkel, Morfill, Moy, Mukherjee, Philip M. D. Severin, Pincet, Ren, Rief, Segal, Sidorova, Sidorova, Strunz, Uhrig, Viani, Wiegand, Wu   +42 more
core   +1 more source

SiO2‐CaOCME/Poly(Tetrahydrofuran)/Poly(Caprolactone) 3D‐Printed Scaffolds Drive Human‐Bone Marrow Stromal Cell Osteogenic Differentiation

Advanced Healthcare Materials, EarlyView.
3D printed hybrid scaffolds combining bioactive silica–calcium chemistry with elastic polymers guide human bone stem cells to form bone. The scaffolds support cell survival, organization, and invasion while releasing osteogenic ions. Together, architecture and composition drive bone‐specific gene expression, extracellular matrix organization, and ...
David R. Sory, Agathe C.M. Heyraud, Julian R. Jones, Sara M. Rankin   +3 more
wiley   +1 more source

Terbium to Quantum Dot FRET Bioconjugates for Clinical Diagnostics: Influence of Human Plasma on Optical and Assembly Properties

Sensors, 2011
Förster resonance energy transfer (FRET) from luminescent terbium complexes (LTC) as donors to semiconductor quantum dots (QDs) as acceptors allows extraordinary large FRET efficiencies due to the long Förster distances afforded.
Niko Hildebrandt, Juan B. Blanco-Canosa, Philip E. Dawson, Michael H. Stewart, Kimihiro Susumu, W. Russ Algar, Frank Morgner, Igor L. Medintz, Daniel Geißler, Stefan Stufler   +9 more
doaj   +1 more source

Streptavidin Promotes DNA Binding and Activation of cGAS to Enhance Innate Immunity

iScience, 2020
Summary: cGAS/STING signaling plays an essential role in sensing cytosolic DNA. cGAS activity is regulated by posttranslational modifications and binding partners. cGAS interactome largely includes mammalian or viral proteins.
Yanqiong Zhang, Zhe Ma, Ying Wang, Joshua Boyer, Guoxin Ni, Liang Cheng, Siyuan Su, Zhigang Zhang, Zhichuan Zhu, Jiayi Qian, Lishan Su, Qi Zhang, Blossom Damania, Pengda Liu   +13 more
doaj   +1 more source

Enhanced Human Antigen‐Specific B Cell Responses Using In Vitro 3D Tonsil Cultures Containing Stromal Cells

Advanced Healthcare Materials, EarlyView.
We developed a fully human 3D tonsil cell culture system incorporating supportive stromal cells that better sustains and activates immune cells than conventional methods. The model generates stronger, more targeted antibody responses to viral antigens and vaccines, providing a physiologically relevant and entirely human platform for studying immune ...
Maaike V. J. Braham, Marlon de Gast, Liubov Babii, Sabine Kruijer, Theo M. Bestebroer, Mathilde Richard, Mathieu Claireaux, S. Marieke van Ham, Jelle de Wit, Cécile A. C. M. van Els, Anja ten Brinke, Marit J. van Gils   +11 more
wiley   +1 more source

Towards HIV Detection: Novel Poly(propylene imine) Dendrimer-Streptavidin Platform for Electrochemical DNA and gp120 Aptamer Biosensors

International Journal of Electrochemical Science, 2014
The detection of sequence - specific DNA and HIV glycoprotein 120 (gp120) based on novel generation 4 poly(propylene imine) dendrimer/streptavidin platform is reported.
Suru V. John, Lia S. Rotherham, Makobetsa Khati, Bhekie B. Mamba, Omotayo A. Arotiba   +4 more
doaj   +1 more source

A ribose-functionalized NAD+ with unexpected high activity and selectivity for protein poly-ADP-ribosylation. [PDF]

, 2019
Nicotinamide adenine dinucleotide (NAD+)-dependent ADP-ribosylation plays important roles in physiology and pathophysiology. It has been challenging to study this key type of enzymatic post-translational modification in particular for protein poly-ADP ...
Chen, Jingwen, Cheng, Qinqin, Dai, Zhefu, Evdokimov, Nikolai M, Lam, Albert T, Louie, Stan G, Lu, Yanran, Pei, Hua, Zhang, Xiao-Nan, Zhang, Yong   +9 more
core   +1 more source

Formation and removal of alkylthiolate self-assembled monolayers on gold in aqueous solutions [PDF]

, 2006
We report the development of novel reagents and approaches for generating recyclable biosensors. The use of aqueous media for the formation of protein binding alkylthiolate monolayers on Au surfaces results in accelerated alkylthiolate monolayer ...
Canaria, Christie A., Fraser, Scott E., Lansford, Rusty, Maloney, James R., Roukes, Michael L., Smith, Jeffrey O., So, Jonathan, Yu, C. J.   +7 more
core   +1 more source

Bifunctional Surfaces With Immobilized Antibodies and Bioactive Peptides Mediate Selective Capture and Proliferation of Endothelial Colony‐Forming Cells

Advanced Healthcare Materials, EarlyView.
A tunable bifunctional surface is developed by combining Fc‐binding peptides and RGD motifs to present oriented antibodies alongside integrin‐mediated signaling. The platform selectively captures endothelial colony‐forming cells while promoting their adhesion and proliferation.
Hugo A. Level, Marc‐Antoine Campeau, Mohamed A. Elkhodiry, Gaétan Laroche, Jean‐François Tanguay, Corinne A. Hoesli   +5 more
wiley   +1 more source

Designed Liquid Crystalline Nanoassemblies From Clinically Validated Polyunsaturated Lipids for Combined Antioxidant, Anti‐Apoptotic, and Neurotrophic Treatments

Advanced Healthcare Materials, EarlyView.
Ionizable lipid nanoparticles target multiple pathological pathways in neurodegeneration. The designed self‐assembled materials undergo a pH‐triggered structural transformation from a cubosome/hexosome coexistence to a hexosome phase, enhancing intracellular delivery of a multi‐target phytochemical formulation. The antioxidant‐loaded pH‐responsive LNPs
Thelma Akanchise, Fucen Luo, Borislav Angelov, Yuru Deng, Angelina Angelova   +4 more
wiley   +1 more source