Results 101 to 110 of about 326,708 (370)

Experimental study of the evanescent-wave photonic sensors response in presence of molecular beacon conformational changes [PDF]

, 2018
An experimental study of the influence of the conformational change suffered by molecular beacon (MB) probes ‐upon the biorecognition of nucleic acid target oligonucleotides over evanescent wave photonic sensors‐ is reported.
Bayer, Bañuls, Casanova-Salas, Conteduca, Dodge, Dubertret, Escorihuela, Escorihuela, Estevez, Fan, García, Goyal, Guo, Gónzalez-Lucas, Heinlein, Hopwood, Huertas, Huertas, Joshi, Kotnala, Li, Li, Liu, Luchansky, Mei, Miao, Muhonen, Patel, Povinelli, Prabhathan, Pérez-López, Rai, Riccelli, Ricciardi, Ruiz-Tórtola, Sassolas, Tortajada-Genaro, Tyagi, Vainrub, Van Lierop, Wang, Wei, Williams, Winrich, Yáñez-Sedeño, Zanchetta, Zheng   +46 more
core   +2 more sources

Co‐expression of HSV‐1 ICP34.5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity

FEBS Open Bio, EarlyView.
ICP34.5 is one of the most important antihost response proteins. The saRNA‐encoding HSV‐1 neurovirulence protein ICP34.5 clearly mediated the eukaryotic initiation factor 2 alpha subunit (eIF2α) dephosphorylation and significant suppression of innate immune responses in vitro, leading to enhanced expression of the saRNA‐encoded gene.
Xuemin Lu, Yabin Wu, Chunye Zhao, Jie Zheng, Shangwu Chen, Yigang Wang, Yulong Xia   +6 more
wiley   +1 more source

Structure-guided design of an engineered streptavidin with reusability to purify streptavidin-binding peptide tagged proteins or biotinylated proteins. [PDF]

PLoS ONE, 2013
Development of a high-affinity streptavidin-binding peptide (SBP) tag allows the tagged recombinant proteins to be affinity purified using the streptavidin matrix without the need of biotinylation.
Sau-Ching Wu, Sui-Lam Wong
doaj   +1 more source

Flap Endonuclease Disengages Dna2 Helicase/Nuclease from Okazaki Fragment Flaps [PDF]

, 2006
Okazaki fragments contain an initiator RNA/DNA primer that must be removed before the fragments are joined. In eukaryotes, the primer region is raised into a flap by the strand displacement activity of DNA polymerase {delta}.
Bambara, Robert A., Campbell, Judith L., Stewart, Jason A.   +2 more
core   +1 more source

Biologically‐Inspired Melt Electrowriting for the Generation of Highly Biomimetic Functional Myocardium

Advanced Functional Materials, EarlyView.
In this work, melt electrowriting is used to fabricate a 3D printed scaffold design that generates engineered cardiac tissues with in‐plane contraction, mimicking natural myocardium. It is shown that these tissues display advanced maturation and functionality.
Olalla Iglesias‐García, María Flandes‐Iparraguirre, Pilar Montero‐Calle, Ricardo M. Rosales, Asier Ullate‐Agote, Andrea Sánchez‐Bueno, Eduardo Larequi, Ilazki Anaut‐Lusar, Nicolás Laita, Aida Oliván‐Viguera, Elena Iglesias, Gloria Abizanda, Patxi San Martín‐Úriz, Paula Aguirre‐Ruiz, Xabier L. Aranguren, Manuel García de Yébenes, Juan José Gavira, Miguel Ángel Martínez, Estefanía Peña, Manuel Doblaré, Elena M. de‐Juan‐Pardo, Esther Pueyo, Felipe Prosper, Manuel M. Mazo Vega   +23 more
wiley   +1 more source

Terbium to Quantum Dot FRET Bioconjugates for Clinical Diagnostics: Influence of Human Plasma on Optical and Assembly Properties

Sensors, 2011
Förster resonance energy transfer (FRET) from luminescent terbium complexes (LTC) as donors to semiconductor quantum dots (QDs) as acceptors allows extraordinary large FRET efficiencies due to the long Förster distances afforded.
Niko Hildebrandt, Juan B. Blanco-Canosa, Philip E. Dawson, Michael H. Stewart, Kimihiro Susumu, W. Russ Algar, Frank Morgner, Igor L. Medintz, Daniel Geißler, Stefan Stufler   +9 more
doaj   +1 more source

Falsely diagnosed thyrotoxicosis caused by anti-streptavidin antibodies and pre-wash procedures

Thyroid Research, 2021
Background Anti-streptavidin antibodies are causal determinants of analytical interference during Thyroid function tests, and numerous reports have detailed such interference, with anti-streptavidin antibodies attracting attention.
Takuya Ishikawa, Hiroyuki Sakai, Tokutaro Itaya, Suwanai Hirotsugu, Jumpei Shikuma, Takashi Miwa, Ryo Suzuki, Masato Odawara   +7 more
doaj   +1 more source

Alleviation of Aging‐Related Hallmarks in a Mouse Model of Progeria via a Nanoparticle‐Based Artificial Transcription Factor

Advanced Functional Materials, EarlyView.
Oct4‐nanoscript, a biomimetic nanoparticle‐based artificial transcription factor, precisely regulates cellular rejuvenation by activating Oct4 target genes, restoring epigenetic marks, and reducing DNA damage. In a progeria model, it effectively rescued aging‐associated pathologies and extended lifespan.
Hongwon Kim, Junyeop Kim, Euiyeon Lee, Brandon Conklin, Yannan Hou, Sumin Kim, Yerim Hwang, Ki‐Bum Lee, Jongpil Kim   +8 more
wiley   +1 more source

Monodisperse measurement of the biotin-streptavidin interaction strength in a well-defined pulling geometry

PLoS ONE, 2017
The widely used interaction of the homotetramer streptavidin with the small molecule biotin has been intensively studied by force spectroscopy and has become a model system for receptor ligand interaction.
Steffen M. Sedlak, M. S. Bauer, C. Kluger, Leonard C. Schendel, L. Milles, Diana A Pippig, H. Gaub   +6 more
semanticscholar   +1 more source

Strategies to Design and Optimize Artificial Antigen‐Presenting Cells for T Cell Expansion in Cancer Immunotherapy

Advanced Functional Materials, EarlyView.
This review highlights recent advances in engineering artificial antigen‐presenting cells (aAPCs) as alternatives to dendritic cells for T cell expansion. Key design principles inspired by the immunological synapse are discussed, with emphasis on strategies for polyclonal and antigen‐specific T cell expansion.
Nguyen Thi Nguyen, Yu Seok Youn
wiley   +1 more source