Results 191 to 200 of about 318,069 (262)

Multimodal Multiorgan‐on‐a‐Chip Platform for Probing Liver‐Tumor Interactions and Advancing Prodrug Screening

Advanced Science, EarlyView.
This work introduces a multimodal multi‐OoC platform that overcomes current limitations in liver‐tumor interaction studies and prodrug screening. By integrating dynamic microfluidic circuits, electrochemical sensing, and mass analysis, this platform enables non‐invasive, longitudinal monitoring of drug metabolism and hepatotoxicity, offering a ...
Dan Wang, Yisong Huang, Weijian Zhao, Tian Chen, Siyu Bai, Denghui Guo, Mengying Wang, Yaoyao Zhao, Liang Zhao, Guangsheng Guo, Xiayan Wang   +10 more
wiley   +1 more source

SplitTurboID mapping of dimeric protein phosphatase complex interactomes. [PDF]

iScience
Rajkumar A, Gaudreau-Lapierre A, Anthony CLF, Nguyen V, Ooi S, Campuzano D, Trinkle-Mulcahy L.   +6 more
europepmc   +1 more source

Programmable RNA N6,2´‐O‐Dimethyladenosine Editing

Advanced Science, EarlyView.
ABSTRACT N6,2’‐O‐dimethyladenosine (m6Am) is a prevalent RNA modification located at the first transcribed nucleotide adjacent to the 5′ cap of mRNAs, where it has been implicated in gene regulation. However, the lack of methods for precise, transcript‐specific manipulation of m6Am has limited its functional dissection.
Yang Li, Xiangmin Tan, Yaran Liu, Yongquan He, Bo Yuan, Ping Wang, Guangzhi Ma, Mengzhe Guo, Jian Zhou, Qiang Sun   +9 more
wiley   +1 more source

NAD+ capping of sibD transcripts in E. coli is mediated by its minimal promoter and enhanced by ppGpp. [PDF]

Nucleic Acids Res
Liu W, Hu K, Nie S, Feng Z, Li Q, Zhang H, Liang S, Peng J, Zhang S, Cai Z, Xia Y.   +10 more
europepmc   +1 more source

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

Advanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

Scope of Microbial Transglutaminase for Site-Specific and Oriented Immobilization of Native Antibodies from Various Host Species. [PDF]

Langmuir
Beitello E, Osei K, Breausche FE, Friesen JA, Driskell JD.   +4 more
europepmc   +1 more source

Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis

Advanced Science, EarlyView.
In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
wiley   +1 more source

Advancing Lateral Flow Detection in CRISPR/Cas12a Systems Through Rational Understanding and Design Strategies of Reporter Interactions. [PDF]

Biosensors (Basel)
Safenkova IV, Kamionskaya MV, Sotnikov DV, Biketov SF, Zherdev AV, Dzantiev BB.   +5 more
europepmc   +1 more source

Remote Magnetomechanical Neuromodulation Uncovers Therapeutic Mechanisms for Alleviating Parkinsonian Symptoms in Freely Moving Mice

Advanced Science, EarlyView.
Magnetomechanical neuromodulation using magnetic nanodiscs enables remote activation of neurons. In a hemiparkinsonian mouse model, alternating magnetic fields actuate the nanodiscs to generate torque that opens mechanosensitive ion channels within the subthalamic nucleus, thereby modulating basal ganglia motor circuitry.
Anouk Wolters, Lorenzo Signorelli, Christian Herff, Sophia Gimple, Renzo Riemens, Gunter Kenis, Kim Rijkers, Hamed Shabani, Jyh‐Jang Sun, Yasin Temel, Hans Clusmann, Danijela Gregurec, Sarah‐Anna Hescham   +12 more
wiley   +1 more source

Protocol for identifying surface membrane proteins and their associated proteome from mouse cortical neuron cultures by in situ biotinylation. [PDF]

STAR Protoc
Shi L, Bagchi P, Nguyen GN, Faundez V, Bassell GJ.   +4 more
europepmc   +1 more source