Results 41 to 50 of about 315,552 (285)

Monovalent Streptavidin that Senses Oligonucleotides [PDF]

Angewandte Chemie, 2013
We report a straightforward chemical route to monovalent streptavidin, a valuable reagent for imaging. The one-step process is based on a (tris)biotinylated-oligonucleotide blocking three of streptavidin’s four biotin binding sites. Further, the complex is highly sensitive to single-base differences - whereby perfectly matched oligonucleotides trigger ...
Steven K, Taylor, Jingxian, Wang, Natasa, Kostic, Milan N, Stojanovic   +3 more
openaire   +2 more sources

Reversible positioning of single molecules inside zero-mode waveguides [PDF]

, 2014
We have developed a hybrid nanopore/zero-mode waveguide device for single-molecule fluorescence and DNA sequencing applications. The device is a freestanding solid-state membrane with sub-5 nm nanopores that reversibly delivers individual biomolecules to
Foquet, Mathieu, Korlach, Jonas, Larkin, Joseph, Turner, Stephen W., Wanunu, Meni   +4 more
core   +2 more sources

Insights into the structure of mature streptavidin C1 from Streptomyces cinnamonensis reveal the self-binding of the extension C-terminal peptide to biotin-binding sites

IUCrJ, 2021
The members of the avidin protein family are well known for their high affinity towards d-biotin and their structural stability. These properties make avidins a valuable tool for various biotechnological applications.
Byeong Jun Jeon, Sulhee Kim, Min-Seok Kim, Ji-Ho Lee, Beom Seok Kim, Kwang Yeon Hwang   +5 more
doaj   +1 more source

Optoelectronic sensitization of carbon nanotubes by CdTe nanocrystals [PDF]

, 2009
We investigate the photoconductance of single-walled carbon nanotube-nanocrystal hybrids. The nanocrystals are bound to the nanotubes via molecular recognition.
Carmeli, I., Hartschuh, Achim, Holleitner, Alexander W., Kotthaus, Jörg P., Vieyra, H. A., Zebli, B.   +5 more
core   +2 more sources

An electrochemical aptasensor for detection of Helicobacter pylori based on AuNPs and AgNPs-GO nanoparticles

Frontiers in Bioengineering and Biotechnology
Helicobacter pylori is one of the main causes of gastritis and gastric ulcer. Early detection of H. pylori is of great significance for the prevention of gastric cancer.
Xiaojuan You, Xiaojuan You, Xiaojuan You, Xiaojuan You, Mingyi Shao, Mingyi Shao, Huadong Wang, Rui Zhu, Xinwei Liu, Lei Dong, Yuesheng Gong, Yongwei Li, Yongwei Li   +12 more
doaj   +1 more source

The use of streptavidin conjugates as immunoblot loading controls and mitochondrial markers for use with Dictyostelium discoideum

BioTechniques, 2013
The loading controls used for quantitative immunoblotting of mammalian proteins are not appropriate for use with Dictyostelium discoideum. Actin levels, for example, change greatly during Dictyostelium development.
Andrew J. Davidson, Jason S. King, Robert H. Insall   +2 more
doaj   +1 more source

Streptavidin-Conjugated DNA for the Boronate Affinity-Based Detection of Poly(ADP-Ribose) Polymerase-1 with Improved Sensitivity

Biosensors, 2023
This work reports the development of a fluorescence method for the detection of poly(ADP-ribose) polymerase-1 (PARP1), in which a phenylboronic acid-modified fluorescein isothiocyanate dye (FITC-PBA) was used to recognize the formed poly(ADP-ribose) (PAR)
Fengli Gao, Gang Liu, Yishu Qiao, Xiuwen Dong, Lin Liu   +4 more
doaj   +1 more source

Aptamer-based cocaine assay using a nanohybrid composed of ZnS/Ag2Se quantum dots, graphene oxide and gold nanoparticles as a fluorescent probe [PDF]

, 2020
Authors report on a new fluoro-graphene-plasmonic nanohybrid aptamer-based fluorescent nanoprobe for cocaine. To construct the nanoprobe, newly synthesized glutathione-capped ZnS/Ag 2Se quantum dots (QDs) were first conjugated to graphene oxide (GO) to ...
Abdolvand, Amin, Adegoke, Oluwasesan, Nic Daeid, Niamh, Pereira-Barros, Magda A., Zolotovskaya, Svetlana   +4 more
core   +2 more sources

Detection of protein-protein interactions using nonimmune IgG and BirA-mediated biotinylation

BioTechniques, 2010
Detection of protein-protein interactions in cells is crucial for understanding the biological functions of proteins, including their roles in signal transduction.
Cai Huang, Ken Jacobson
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source