Results 41 to 50 of about 326,708 (370)
Electrophoretic behavior of streptavidin complexed to a biotinylated probe : A functional screening assay for biotin-binding proteins [PDF]
, 2005 The biotin-binding protein streptavidin exhibits a high stability against thermal denaturation, especially when complexed to biotin. Herein we show that, in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), streptavidin is stabilized ...
Humbert, Nicolas +2 more
core +1 more source
Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution
Nature Communications, 2018 The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 Å resolution.
Xiao Fan +8 more
semanticscholar +1 more source
Nanomaterials, 2019 The chemical modification, or functionalization, of the surfaces of nanomaterials is a key step to achieve biosensors with the best sensitivity and selectivity. The surface modification of biosensors usually comprises several modification steps that have
Laura Pol +5 more
doaj +1 more source
IUCrJ, 2021 The members of the avidin protein family are well known for their high affinity towards d-biotin and their structural stability. These properties make avidins a valuable tool for various biotechnological applications.
Byeong Jun Jeon +5 more
doaj +1 more source
ACS Omega, 2019 Overconsumption of biotin (5–100 mg daily) as a supplement by the general population poses a significant problem for clinical immunoassays (IAs) based on biotin–streptavidin (SA) interactions. This affinity pair has been exploited in immunoassays because
J. Luong, S. K. Vashist
semanticscholar +1 more source
Influences of retinal axons on the cultural substrate containing biotin-conjugated chondroitin sulfate in vitro [PDF]
, 2010 Although chondroitin sulfate (CS) is known to act as an inhibitory axon guidance cue, retinal axons show substantial growth on a culture substrate containing CS.
Ando Satoru +4 more
core +1 more source
Aptamer-Based Nanoporous Anodic Alumina Interferometric Biosensor for Real-Time Thrombin Detection
Sensors, 2019 Aptamer biosensors are one of the most powerful techniques in biosensing. Achieving the best platform to use in aptamer biosensors typically includes crucial chemical modifications that enable aptamer immobilization on the surface in the most efficient ...
Laura Pol +3 more
doaj +1 more source
Magnetic particle plug-based assays for biomarker analysis [PDF]
, 2016 Conventional immunoassays offer selective and quantitative detection of a number of biomarkers, but are laborious and time-consuming. Magnetic particle-based assays allow easy and rapid selection of analytes, but still suffer from the requirement of ...
Pamme, Nicole +2 more
core +3 more sources
Production and Bioseparation Applications of Polyhydroxyalkanoate Nano-Granules Functionalized with Streptavidin. [PDF]
MicroorganismsRapidly growing industrial biotechnology and bio-manufacturing require simple and cost-effective bioseparation tools. A novel strategy of bioseparation based on the streptavidin-decorated polyhydroxyalkanoate (PHA) nano-granules was developed in this ...
Zhang Y, Zhao J, Guo H, Lu X, Tan D.
europepmc +2 more sources
Selection of DNA nanoparticles with preferential binding to aggregated protein target. [PDF]
, 2016 High affinity and specificity are considered essential for affinity reagents and molecularly-targeted therapeutics, such as monoclonal antibodies.
De Maere, Elisabeth +10 more
core +1 more source