Results 51 to 60 of about 326,708 (370)

Streptavidin-Conjugated DNA for the Boronate Affinity-Based Detection of Poly(ADP-Ribose) Polymerase-1 with Improved Sensitivity

Biosensors, 2023
This work reports the development of a fluorescence method for the detection of poly(ADP-ribose) polymerase-1 (PARP1), in which a phenylboronic acid-modified fluorescein isothiocyanate dye (FITC-PBA) was used to recognize the formed poly(ADP-ribose) (PAR)
Fengli Gao, Gang Liu, Yishu Qiao, Xiuwen Dong, Lin Liu   +4 more
doaj   +1 more source

RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes. [PDF]

, 2017
Synthetic biology and metabolic engineering seek to re-engineer microbes into living foundries for the production of high value chemicals. Through a design-build-test cycle paradigm, massive libraries of genetically engineered microbes can be constructed
Abate, Adam, Abatemarco, Joseph, Alper, Hal, Hassouneh, Wafa, Lin, Jyun-Liang, Liu, Leqian, Sarhan, Maen, Wagner, James, Yuan, Shuo-Fu   +8 more
core   +1 more source

High-Yield Characterization of Single Molecule Interactions with DeepTip^TM Atomic Force Microscopy Probes

Molecules, 2022
Single molecule interactions between biotin and streptavidin were characterized with functionalized DeepTipTM probes and used as a model system to develop a comprehensive methodology for the high-yield identification and analysis of single molecular ...
Daniel Corregidor, Raquel Tabraue, Luis Colchero, Rafael Daza, Manuel Elices, Gustavo V. Guinea, José Pérez-Rigueiro   +6 more
doaj   +1 more source

Targeting extracellular DNA to deliver IGF-1 to the injured heart. [PDF]

, 2014
There is a great need for the development of therapeutic strategies that can target biomolecules to damaged myocardium. Necrosis of myocardium during a myocardial infarction (MI) is characterized by extracellular release of DNA, which can serve as a ...
Brown, Milton, Cabigas, E, Che, Pao-lin, Dasari, Madhuri, Davis, Michael, Khan, Raffay, Martinez, Mario, Murthy, Niren, Pendergrass, Karl, Sy, Jay   +9 more
core   +2 more sources

Effects of High-Biotin Sample Interference on Antibody Concentrations in Sandwich Immunoassays

Vaccines, 2023
The use of antimicrobial growth promoters (AGPs) is banned because of problems associated with drug residues in animal products and increased bacterial resistance. The immunization of chickens with specific antigens is a promising strategy for generating
Geraldo Balieiro Neto, Jair Rodini Engracia Filho, Fabio Enrique Lemos Budino, Acyr Wanderley de Paula Freitas, Weber Vilas Boas Soares   +4 more
doaj   +1 more source

Detailed characterization of the solution kinetics and thermodynamics of biotin, biocytin and HABA binding to avidin and streptavidin

PLoS ONE, 2019
The high affinity (KD ~ 10−15 M) of biotin for avidin and streptavidin is the essential component in a multitude of bioassays with many experiments using biotin modifications to invoke coupling.
Roberto F. Delgadillo, T. Mueser, K. Zaleta-Rivera, Katie A. Carnes, José González‐Valdez, L. Parkhurst   +5 more
semanticscholar   +1 more source

Controlling ligand surface density on streptavidin-magnetic particles by a simple, rapid, and reliable chemiluminescent test [PDF]

, 2018
International ...
Bouzas Ramos, Diego, Costa Fernández, José Manuel, Gagey Eilstein, Nathalie, Pons, Kelly, Ruiz Encinar, Jorge, Trapiella Alfonso, Laura, Tsatsaris, Vassilis   +6 more
core   +1 more source

Dynamin- and Rab5-Dependent Endocytosis of a Ca²⁺-Activated K⁺ Channel, KCa2.3 [PDF]

, 2012
Regulation of the number of ion channels at the plasma membrane is a critical component of the physiological response. We recently demonstrated that the Ca2+-activated K+ channel, KCa2.3 is rapidly endocytosed and enters a Rab35- and EPI64C-dependent ...
Agustin Guerrero-Hernandez, Claudia A. Bertuccio, Corina M. Balut, Daniel C. Devor, Simon C. Watkins, Yajuan Gao   +5 more
core   +7 more sources

A DNA Electrochemical Sensor via Terminal Protection of Small-Molecule-Linked DNA for Highly Sensitive Protein Detection

Biosensors, 2021
The qualitative and quantitative determination of marker protein is of great significance in the life sciences and in medicine. Here, we developed an electrochemical DNA biosensor for protein detection based on DNA self-assembly and the terminal ...
Ping Ouyang, Chenxin Fang, Jialun Han, Jingjing Zhang, Yuxing Yang, Yang Qing, Yubing Chen, Wenyan Shang, Jie Du   +8 more
doaj   +1 more source

Streptavidin-conjugated gold nanoclusters as ultrasensitive fluorescent sensors for early diagnosis of HIV infection

Science Advances, 2018
GNCIA allows a sensitive and selective detection of HIV-1 infections with a sensitivity of 5 pg/ml. We have engineered streptavidin-labeled fluorescent gold nanoclusters to develop a gold nanocluster immunoassay (GNCIA) for the early and sensitive ...
A. Kurdekar, L. A. Avinash Chunduri, C. Sai Manohar, M. Haleyurgirisetty, Indira K. Hewlett, K. Venkataramaniah   +5 more
semanticscholar   +1 more source