Results 71 to 80 of about 326,708 (370)

miRNA‐29 regulates epidermal and mesenchymal functions in skin repair

FEBS Letters, EarlyView.
miRNA‐29 inhibits cell‐to‐cell and cell‐to‐matrix adhesion by silencing mRNA targets. Adhesion is controlled by complex interactions between many types of molecules coded by mRNAs. This is crucial for keeping together the layers of the skin and for regenerating the skin after wounding.
Lalitha Thiagarajan, Rosa Sanchez‐Alvarez, Chiho Kambara, Poojitha Rajasekar, Yuluang Wang, François Halloy, Jonathan Hall, Hans‐Jürgen Stark, Iris Martin, Petra Boukamp, Svitlana Kurinna   +10 more
wiley   +1 more source

Uniform Orientation of Biotinylated Nanobody as an Affinity Binder for Detection of Bacillus thuringiensis (Bt) Cry1Ac Toxin

Toxins, 2014
Nanobodies are the smallest natural fragments with useful properties such as high affinity, distinct paratope and high stability, which make them an ideal tool for detecting target antigens.
Min Li, Min Zhu, Cunzheng Zhang, Xianjin Liu, Yakun Wan   +4 more
doaj   +1 more source

3.1Å structure of yeast RNA polymerase II elongation complex stalled at a cyclobutane pyrimidine dimer lesion solved using streptavidin affinity grids

bioRxiv, 2019
Despite significant advances in all aspects of single particle cryo-electron microscopy (cryo-EM), specimen preparation still remains a challenge. During sample preparation, macromolecules interact with the air-water interface, which often leads to ...
I. Lahiri, Jun Xu, B. Han, Juntaek Oh, Dong Wang, F. DiMaio, A. Leschziner   +6 more
semanticscholar   +1 more source

Diamagnetic repulsion of particles for multilaminar flow assays [PDF]

, 2015
© The Royal Society of Chemistry. We demonstrate diamagnetic repulsion forces for performing continuous multilaminar flow assays on particles based on their intrinsic properties and with a simple setup. The platform could be applied to sandwich assays on
Elders, Luke T., Pamme, Nicole, Peyman, Sally A., Tarn, Mark D.   +3 more
core   +1 more source

STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence

FEBS Letters, EarlyView.
Heart failure with preserved ejection fraction (HFpEF) accounts for half of the heart failure cases. It is characterised by microvascular dysfunction, associated with reduced pericyte coverage and diminished STAT3 expression in pericytes. Loss of STAT3 impairs pericyte adhesion, promotes senescence, and activates a pro‐fibrotic gene program.
Leah Rebecca Vanicek, Ariane Fischer, Mariano Ruz Jurado, Anita Tamiato, Tara Procida‐Kowalski, Jochen Wilhelm, Dennis Hecker, Maximilian Merten, Felicitas Escher, Badder Kattih, Valentina Puntmann, David John, Marcel H. Schulz, Eike Nagel, Stefanie Dimmeler, Guillermo Luxán   +15 more
wiley   +1 more source

Synthesis of Biotin Linkers with the Activated Triple Bond Donor [p-(N-propynoylamino)toluic Acid] (PATA) for Efficient Biotinylation of Peptides and Oligonucleotides

Molecules, 2012
Biotin is an important molecule for modern biological studies including, e.g., cellular transport. Its exclusive affinity to fluorescent streptavidin/avidin proteins allows ready and specific detection.
Martina Jezowska, Joanna Romanowska, Burcu Bestas, Ulf Tedebark, Malgorzata Honcharenko   +4 more
doaj   +1 more source

Selective targeting of proteins by hybrid polyoxometalates: Interaction between a bis-biotinylated hybrid conjugate and avidin [PDF]

, 2018
The Keggin-type polyoxometalate [\u3b3-SiW10O36]8 12 was covalently modified to obtain a bis-biotinylated conjugate able to bind avidin. Spectroscopic studies such as UV-vis, fluorimetry, circular dichroism, coupled to surface plasmon resonance technique
Alessandra Saccani, Alessandro Cazzolaro, Disma Mastrogiacomo, Elisa Lubian, Fabrizio Mancin, Gloria Modugno, Livia Giotta, Ludovico Valli, Marcella Bonchio, Mauro Carraro, Silke Krol, Silke Krol, Valeria A. Zamolo   +12 more
core   +2 more sources

The quaternary structure of streptavidin in urea

Journal of Biological Chemistry, 1991
We report on the interactions of urea and guanidinium salts with streptavidin. Gel filtration chromatography in 0, 4, 6, and 7 M urea indicates that the streptavidin tetramer remains intact in urea. Biotin alters the electrophoretic mobility of streptavidin whether or not 6 M urea is present.
M Wilchek, Edward A. Bayer, Paul M. Horowitz, Gary P. Kurzban   +3 more
openaire   +3 more sources

Identification of functional and diverse circulating cancer‐associated fibroblasts in metastatic castration‐naïve prostate cancer patients

Molecular Oncology, EarlyView.
Cancer‐associated fibroblasts (CAFs) promote cancer growth, invasion (metastasis), and drug resistance. Here, we identified functional and diverse circulating CAFs (cCAFs) in patients with metastatic prostate cancer (mPCa). cCAFs were found in higher numbers and were functional and diverse in mPCa patients versus healthy individuals, suggesting their ...
Richell Booijink, Leon W. M. M. Terstappen, Eshwari Dathathri, Khrystany Isebia, Jaco Kraan, John Martens, Ruchi Bansal   +6 more
wiley   +1 more source

Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin

Nature Communications, 2016
The advent of super-resolution imaging (SRI) has created a need for optimized labelling strategies. We present a new method relying on fluorophore-conjugated monomeric streptavidin (mSA) to label membrane proteins carrying a short, enzymatically ...
Ingrid Chamma, M. Letellier, C. Butler, Béatrice Tessier, K. Lim, I. Gauthereau, D. Choquet, J. Sibarita, Sheldon Park, Matthieu Sainlos, O. Thoumine   +10 more
semanticscholar   +1 more source