Results 91 to 100 of about 32,771 (219)
Numerical Simulation of Stress Field in Laser Cladded Gate of Flat Gate Valve
As a key member of gate valve, the abrasion and corrosion of the gate can severely hamper the application of flat gate valve in the oil industry.Based on thermal elastoplastic theory, laser cladding of the gate with Ni-based alloy was simulated by means ...
Cai Chunbo +3 more
doaj
Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho +4 more
wiley +1 more source
Design method and application of temporary plugging by fiber and diverting acid fracturing
In order to solve the problem of quantitative design of fiber in fiber temporary plugging and diverting acid fracturing, a total stress field model considering the initial artificial fracture, adjacent well artificial fracture, pore pressure change ...
Zhifeng LUO +5 more
doaj
As a researcher engaging in the field of oxidative stress
The awful earthquake that attacked Northeast part of Japan on 11th March 2011 caused a massive tsunami to surge against the coastal place at Kanto and Tohoku area. Besides, it ran over the serious damage of the Fukushima Daiichi Nuclear Power Plant located at the coastal place of north Kanto area.
openaire +3 more sources
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source

