Results 71 to 80 of about 234,776 (300)

Structure elucidation of colibactin [PDF]

, 2019
AbstractColibactin is a gut microbiome metabolite of unknown structure that has been implicated in colorectal cancer formation. Several studies now suggest that the tumorgenicity of colibactin derives from interstrand cross-linking of host DNA. Here we use a combination of genetics, isotope labeling, tandem MS, and chemical synthesis to deduce the ...
Xue, Mengzhao, Kim, Chung Sub, Healy, Alan R., Wernke, Kevin M., Wang, Zhixun, Frischling, Madeline C., Shine, Emilee E., Wang, Weiwei, Herzon, Seth B., Crawford, Jason M.   +9 more
openaire   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

How does iron interact with sporopollenin exine capsules? An X-ray absorption study including microfocus XANES and XRF imaging [PDF]

, 2014
Sporopollenin exine capsules (SECs) derived from plant spores and pollen grains have been proposed as adsorption, remediation and drug delivery agents. Despite many studies there is scant structural data available.
Archibald, Stephen J., Atkin, Stephen L., Bras, Wim, Diego-Taboada, Alberto, Mackenzie, Grahame, Mosselmans, J. Frederick W., Nikitenko, Sergey, Quinn, Paul D., Thomas, Michael F., Young, Nigel A.   +9 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

An efficient computer-aided structural elucidation strategy for mixtures using an iterative dynamic programming algorithm

Journal of Cheminformatics, 2017
The identification of chemical structures in natural product mixtures is an important task in drug discovery but is still a challenging problem, as structural elucidation is a time-consuming process and is limited by the available mass spectra of known ...
Bo-Han Su, Meng-Yu Shen, Yeu-Chern Harn, San-Yuan Wang, Alioune Schurz, Chieh Lin, Olivia A. Lin, Yufeng J. Tseng   +7 more
doaj   +1 more source

Structure elucidation of ficellomycin.

The Journal of Antibiotics, 1989
The structure of ficellomycin, an antibiotic previously discovered by Argoudelis et al., is elucidated as valyl-2-[4-guanidyl-1-azabicyclo[3.1.0]hexan-2-yl]glycine (1) by NMR, MS, and derivatization studies. The 1-azabicyclo[3.1.0]hexane moiety in 1 represents an unusual ring system making ficellomycin a unique natural product compound.
M S, Kuo, D A, Yurek, S A, Mizsak
openaire   +3 more sources

Mass spectrometry-based fragmentation as an identification tool in lignomics [PDF]

, 2010
The ensemble of all phenolics for which the biosynthesis is coregulated with lignin biosynthesis, i.e., metabolites from the general phenylpropanoid, monolignol, and (neo)-lignan biosynthetic pathways and their derivatives, as well as the lignin ...
Akiyama, Takuya, Boerjan, Wout, Dima, Oana, Goeminne, Geert, Inzé, Dirk, Kim, Hoon, Lu, Fachuang, Messens, Eric, Morreel, Kris, Niculaes, Claudiu, Ralph, John, Vanholme, Ruben   +11 more
core   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Chromatographic Separation of the Codonocarpine Type Alkaloids from the Root Bark of Capparis decidua

Bio-Protocol, 2017
Various parts of the caper tree Capparis decidua have found application in traditional medicine. The isolation and structural elucidation of the codonocarpine type alkaloids contained in the root bark, however, is not trivial and has probably led to ...
Yvonne Forster, Abdul Ghaffar, Stefan Bienz   +2 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source