Results 41 to 50 of about 191,821 (303)

Identification of subcellular compartments involved in biosynthetic processing of cathepsin D.

Journal of Biological Chemistry, 1992
We have assigned the biosynthetic processing steps of cathepsin D to intracellular compartments which are involved in its transport to lysosomes in HepG2 cells. Cathepsin D was synthesized as a 51-kDa proenzyme. After formation of 51-55-kDa intermediates due to processing of N-linked oligosaccharides, procathepsin D was proteolytically processed to an ...
Rijnboutt, S, Stoorvogel, W, Geuze, H J, Strous, G J   +3 more
openaire   +3 more sources

Uptake and subcellular processing of 59Fe-125I-labelled transferrin by rat liver [PDF]

Biochemical Journal, 1986
The uptake of transferrin and iron by the rat liver was studied after intravenous injection or perfusion in vitro with diferric rat transferrin labelled with 125I and 59Fe. It was shown by subcellular fractionation on sucrose density gradients that 125I-transferrin was predominantly associated with a low-density membrane fraction, of similar density to
E H, Morgan, G D, Smith, T J, Peters
openaire   +2 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Mapping subcellular RNA localization with proximity labeling

Acta Biochimica et Biophysica Sinica
The subcellular localization of RNA is critical to a variety of physiological and pathological processes. Dissecting the spatiotemporal regulation of the transcriptome is key to understanding cell function and fate.
Liu Jiapeng, Zhong Binglin, Li Shuojun, Han Shuo   +3 more
doaj   +1 more source

tRNA Processing and Subcellular Trafficking Proteins Multitask in Pathways for Other RNAs [PDF]

Frontiers in Genetics, 2019
This article focuses upon gene products that are involved in tRNA biology, with particular emphasis upon post-transcriptional RNA processing and nuclear-cytoplasmic subcellular trafficking. Rather than analyzing these proteins solely from a tRNA perspective, we explore the many overlapping functions of the processing enzymes and proteins involved in ...
Anita K. Hopper, Regina T. Nostramo
openaire   +3 more sources

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Steroid hormones and subcellular processes [PDF]

, 1968
Aldosterone administered intraperitoneally induced an increase in the rate of renal RNA synthesis in the rat. A maximum response of 130 percent of control occurred 1.5 hours after injection.
James, Gordon Price
core  

DNA damage-induced translocation of S100A11 into the nucleus regulates cell proliferation

BMC Cell Biology, 2010
Background Proteins are able to react in response to distinct stress stimuli by alteration of their subcellular distribution. The stress-responsive protein S100A11 belongs to the family of multifunctional S100 proteins which have been implicated in ...
Ulbricht Tobias, Murzik Ulrike, Gorsler Theresa, Hentschel Julia, Hemmerich Peter, Melle Christian   +5 more
doaj   +1 more source

Nuclear RNA purification by flow cytometry to study nuclear processes in plants

STAR Protocols, 2021
Summary: The nature of plant tissues has continuously hampered understanding of the spatio-temporal and subcellular distribution of RNA-guided processes.
Belén Moro, Malgorzata Kisielow, Veronica Barragan Borrero, Antoine Bouet, Christopher A. Brosnan, Nicolás G. Bologna   +5 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source