Results 151 to 160 of about 660,873 (335)
FEBS Open Bio, EarlyView.The C. elegans tetraspanin‐7 (tsp‐7) is a homologue of human CD63, which is a negative regulator of autophagy. The C. elegans strain, tm5761, has a dysfunctional (knockout) tsp‐7 gene. When compared to the wild‐type strain, the tm5761 strain shows increased: life‐ and health‐span; thermotolerance, and stress‐induced locomotion.
Brogan Jones +2 more
wiley +1 more source
The Bifidobacterium adolescentis BAD_1527 gene encodes GH43_22 α-L-arabinofuranosidase of AXH-m type
AMB ExpressBifidobacterium adolescentis gene BAD_1527 has previously been suggested to code for a β-xylosidase (Kobayashi et al., Mar Drugs 18:174, 2020). Our detailed investigation of the substrate specificity of the GH43_22 protein using a wide spectrum of ...
Walid Fathallah, Vladimír Puchart
doaj +1 more source
FEBS Open Bio, EarlyView.We introduce an immunohistochemistry method to measure autophagy flux, highlighting the active degradation and recycling of cellular waste. This cost‐effective approach uses tissue samples to track key markers like LC3 and SQSTM1, revealing how cells maintain health or respond to diseases such as cancer. It bridges the gap between research and clinical
Shahla Shojaei +6 more
wiley +1 more source
Investigations of the Chymotrypsin-catalyzed Hydrolysis of Specific Substrates
, 1967 Karl G. Brandt +2 more
openalex +1 more source
Distribution and Substrate Specificity of Benzylpenicillin Acylase [PDF]
, 1963 H. T. Huang, T. Seto, G. M. Shull
openalex +1 more source
FEBS Open Bio, EarlyView.Heterologous expression of membrane transporters in cultured cells is essential for functional characterization, but is sometimes limited by low activity. Our study compares the HDAC inhibitors butyrate, VPA and SAHA to enhance transport activity. We propose to replace butyrate by SAHA: it is equally effective, devoid of repulsive odor, costs less, and
Svenja Flögel +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley +1 more source
The Significance of the Substrate Specificity of T2r+-induced Deoxycytidylate Deaminase
, 1966 Gladys F. Maley, Frank Maley
openalex +1 more source