Results 41 to 50 of about 660,873 (335)
Current understanding of substrate specificity and regioselectivity of LPMOs
Bioresources and Bioprocessing, 2020 Renewable biomass such as cellulose and chitin are the most abundant sustainable sources of energy and materials. However, due to the low degradation efficiency of these recalcitrant substrates by conventional hydrolases, these biomass resources cannot ...
Xiaoli Zhou, Honghui Zhu
doaj +1 more source
Crystal structure and substrate specificity of Drosophila 3,4-dihydroxyphenylalanine decarboxylase. [PDF]
PLoS ONE, 2010 3,4-Dihydroxyphenylalanine decarboxylase (DDC), also known as aromatic L-amino acid decarboxylase, catalyzes the decarboxylation of a number of aromatic L-amino acids.
Qian Han +4 more
doaj +1 more source
Substrate specificity of Ty1 integrase [PDF]
Journal of Virology, 1995 Integration of the Saccharomyces cerevisiae retrotransposon Ty1 requires the element-encoded integrase (IN) protein, which is a component of cytoplasmic virus-like particles (VLPs). Using purified recombinant Ty1 IN and an oligonucleotide integration assay based on Ty1 long terminal repeat sequences, we have compared IN activity on substrates having ...
S P Moore, D J Garfinkel, M Powers
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Diphthamide synthesis is linked to the eEF2‐client chaperone machinery
FEBS Letters, EarlyView.The diphthamide modification of eukaryotic translation elongation factor 2 (eEF2) is important for accurate protein synthesis. We addressed the potential coordination of de novo eEF2 synthesis with simultaneous or subsequent diphthamide modification. Our work reveals that the co‐chaperones Hgh1 and Cpr7, which are known to support folding of nascent ...
Lars Kaduhr +4 more
wiley +1 more source
Substrate Specificity of Peptide Adsorption: A Model Study [PDF]
Phys. Rev. E 73, 020901(1-4)(R) (2006), 2007 Applying the contact density chain-growth algorithm to lattice heteropolymers, we identify the conformational transitions of a nongrafted hydrophobic-polar heteropolymer with 103 residues in the vicinity of a polar, a hydrophobic, and a uniformly attractive substrate. Introducing only two system parameters, the numbers of surface contacts and intrinsic
arxiv +1 more source
Characterization of fungal carbonyl sulfide hydrolase belonging to clade D β‐carbonic anhydrase
FEBS Letters, EarlyView.Here, we performed a functional analysis of the fungal enzymes belonging to clade D of the β‐class carbonic anhydrase family (β‐D‐CA). The β‐D‐CAs in the basidiomycete Gloeophyllum trabeum and the ascomycete Trichoderma harzianum showed very low activity in the hydration of CO2 but exhibited high activity in the hydrolysis of carbonyl sulfide (COS ...
Ryuka Iizuka +6 more
wiley +1 more source
FEBS Letters, EarlyView.The glacier ice worm Mesenchytraeus solifugus survives year‐round at 0 °C. Its ATP6 subunit, which forms a regulatory component of the proton pore in mitochondrial ATP synthase, has a carboxy‐terminal extension not found in any other organism examined to date. Here, we show that fusion of this extension to the homologous AtpB protein in E. coli results
Truman Dunkley +2 more
wiley +1 more source
Probabilistic approach to predicting substrate specificity of methyltransferases.
PLoS Computational Biology, 2014 We present a general probabilistic framework for predicting the substrate specificity of enzymes. We designed this approach to be easily applicable to different organisms and enzymes.
Teresa Szczepińska +7 more
doaj +1 more source
Substrate specificity of ceramide trihexosidase
FEBS Letters, 1972 Artificial substrates such as 4-methylumbelliferyl glycosides and p-nitrophenyl glycosides have been employed commonly to determine the levels of glycosyl hydrolase activities in plasma, urine and tissues [l-7]. They have provided a simple and rapid method for the diagnosis of some lysosomal storage diseases, whereas in other instances inconsistencies ...
Carol A. Mapes, Charles C. Sweeley
openaire +3 more sources
Mitigation of substrate coupling effects in RF switch by localized substrate removal using laser processing [PDF]
arXiv, 2021 With the evolution of radio frequency (RF)/microwave technology, there is a demand for circuits which are able to meet highly challenging RF frontend specifications. Silicon-on-insulator (SOI) technology is one of the leading platforms for upcoming wireless generation. The degradation of performance due to substrate coupling is a key problem to address
arxiv