PCSK1 Mutations and Human Endocrinopathies: From Obesity to Gastrointestinal Disorders. [PDF]
, 2016 Prohormone convertase 1/3, encoded by the PCSK1 gene, is a serine endoprotease that is involved in the processing of a variety of proneuropeptides and prohormones. Humans who are homozygous or compound heterozygous for loss-of-function mutations in PCSK1
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BMC Bioinformatics, 2018 Background Sedolisins are acid proteases that are related to the basic subtilisins. They have been identified in all three superkingdoms but are not ubiquitous, although fungi that secrete acids as part of their lifestyle can have up to six paralogs ...
Facundo Orts, Arjen ten Have
doaj +1 more source
Molecular determinants for subcellular trafficking of the malarial sheddase PfSUB2. [PDF]
, 2013 The malaria merozoite invades erythrocytes in the vertebrate host. Iterative rounds of asexual intraerythrocytic replication result in disease. Proteases play pivotal roles in erythrocyte invasion, but little is understood about their mode of action. The
Aikawa +54 more
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At the nexus of three kingdoms: the genome of the mycorrhizal fungus Gigaspora margarita provides insights into plant, endobacterial and fungal interactions. [PDF]
, 2020 As members of the plant microbiota, arbuscular mycorrhizal fungi (AMF, Glomeromycotina) symbiotically colonize plant roots. AMF also possess their own microbiota, hosting some uncultivable endobacteria.
Amselem, Joëlle +12 more
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Chemically stabilized subtilisins in peptide synthesis [PDF]
, 2008 We have stabilized alcalaseTM and subtilisin Carlsberg (SC) against heat by chemical modification with ethylene glycol bis-succinimidyl succinate (EGNHS), a procedure not previously reported for subtilisins.
Colleary, Sandra, Ó Fágáin, Ciarán
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Molecular Oncology, EarlyView.Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova +25 more
wiley +1 more source
Gluten Immunogenic Peptides as Standard for the Evaluation of Potential Harmful Prolamin Content in Food and Human Specimen [PDF]
, 2018 Gluten is a complex mixture of storage proteins in cereals like wheat, barley, and rye. Prolamins are the main components of gluten. Their high content in proline and glutamine makes them water-insoluble and difficult to digest in the gastrointestinal ...
Cebolla Ramírez, Ángel +3 more
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The hydrolysis of the three clupein components by subtilisin Novo, subtilisin Carlsberg and nitrated subtilisin Carlsberg [PDF]
Carlsberg Research Communications, 1976 The three components of clupein (YI, YII and Z) were hydrolyzed by subtilisin Carlsberg and subtilisin Novo. Subtilisin Novo hydrolyzed all three components much faster than did subtilisin Carlsberg, but components YII and Z were hydrolyzed with the same initial rate and faster than component YI.
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Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Isolation, purification, characterization and applications of serine protease from Bacillus megaterium [PDF]
, 2010 Bacillus megaterium isolated from poultry farm soil was identified by standard biochemical tests and screened for the production of serine protease. Production of serine protease was done using 5 different medias by varying the type of amino acid added ...
Deepa, Deepa +4 more
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