Probing the crucial role of Leu31 and Thr33 of the Bacillus pumilus CBS alkaline protease in substrate recognition and enzymatic depilation of animal hide. [PDF]
PLoS ONE, 2014 The sapB gene, encoding Bacillus pumilus CBS protease, and seven mutated genes (sapB-L31I, sapB-T33S, sapB-N99Y, sapB-L31I/T33S, sapB-L31I/N99Y, sapB-T33S/N99Y, and sapB-L31I/T33S/N99Y) were overexpressed in protease-deficient Bacillus subtilis DB430 and
Nadia Zaraî Jaouadi +10 more
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Cold Adaptation of a Mesophilic Subtilisin-like Protease by Laboratory Evolution [PDF]
, 2000 Enzymes isolated from organisms native to cold environments generally exhibit higher catalytic efficiency at low temperatures and greater thermosensitivity than their mesophilic counterparts.
Arnold, Frances H. +2 more
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A new subfamily of fungal subtilases: structural and functional analysis of a Pleurotus ostreatus member [PDF]
, 2005 Pleurotus ostreatus produces several extracellular proteases which are believed to be involved in the regulation of the ligninolytic activities of this fungus. Recently, purification and characterization of the most abundant P.
FARACO, VINCENZA +5 more
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Food Technology and Biotechnology, 2018 This paper presents the results of the study on the production of protease by Bacillus luteus H11 isolated from an alkaline soda lime. B. luteus H11 was identified as an alkalohalophilic bacterium, and its extracellular serine endoprotease also showed an
Agnieszka Kalwasińska +4 more
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Pharmaceutically modified subtilisins withstand acidic conditions and effectively degrade gluten in vivo [PDF]
, 2019 Detoxification of gluten immunogenic epitopes is a promising strategy for the treatment of celiac disease. Our previous studies have shown that these epitopes can be degraded in vitro by subtilisin enzymes derived from Rothia mucilaginosa, a natural ...
Darwish, Ghassan +4 more
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Transcription of Bacillus subtilis subtilisin and expression of subtilisin in sporulation mutants [PDF]
Journal of Bacteriology, 1988 The start point for transcription of the subtilisin (aprE) gene was determined by primer extension analysis and was found to be at a point significantly different from that identified in a previously published report (S. L. Wong, C. W. Price, D. S. Goldfarb, and R. H. Doi, Proc. Natl. Acad. Sci. USA 81:1184-1188, 1984).
E, Ferrari +3 more
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BMC Microbiology, 2002 Background Exported proteases are commonly associated with virulence in bacterial pathogens, yet there is a paucity of information regarding their role in Mycobacterium tuberculosis.
Ehlers Mario RW +4 more
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Identification of functionally important microRNAs from rice inflorescence at heading stage of a qDTY4.1-QTL bearing Near Isogenic Line under drought conditions. [PDF]
PLoS ONE, 2017 A cross between IR64 (high-yielding but drought-susceptible) and Aday Sel (drought-tolerant) rice cultivars yielded a stable line with enhanced grain yield under drought screening field trials at International Rice Research Institute.
Boon Huat Cheah +4 more
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Strategies towards the functionalization of subtilisin E from bacillus subtilis for wool finishing applications [PDF]
, 2008 Subtilisin E is an alkaline serine protease secreted by the Gram positive bacterium Bacillus subtilis and widely used in industry as a biocatalyst for various processes. The most common application of subtilisins is in laundry detergents. However, due to
Araújo, Rita +2 more
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Development of Versatile Vectors for Heterologous Expression in Bacillus
Microorganisms, 2018 The discovery of new enzymes for industrial application relies on a robust discovery pipeline. Such a pipeline should facilitate efficient molecular cloning, recombinant expression and functional screening procedures.
Øivind Larsen +1 more
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