Results 51 to 60 of about 1,319 (144)

Comparative CRISPR type III-based knockdown of essential genes in hyperthermophilic Sulfolobales and the evasion of lethal gene silencing [PDF]

, 2020
CRISPR type III systems, which are abundantly found in archaea, recognize and degrade RNA in their specific response to invading nucleic acids. Therefore, these systems can be harnessed for gene knockdown technologies even in hyperthermophilic archaea to
Baum, B, Schleper, C, Bläsi, U, Tarrason Risa, G, Fouqueau, T, Werner, F, Zink, IA   +6 more
core  

Data_Sheet_1_Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic CO2 Fixation in Metallosphaera sedula.PDF

, 2020
Autotrophic Crenarchaeota use two different cycles for carbon dioxide fixation. Members of the Sulfolobales use the 3-hydroxypropionate/4-hydroxybutyrate (HP/HB) cycle, whereas Desulfurococcales and Thermoproteales use the dicarboxylate/4-hydroxybutyrate
Ivan A. Berg (8558781), Harald Huber (211414), Li Liu (75607)   +2 more
core   +1 more source

Crystal structure of the translation recovery factor Trf from Sulfolobus solfataricus

FEBS Open Bio, 2020
During translation initiation, the heterotrimeric archaeal translation initiation factor 2 (aIF2) recruits the initiator tRNAi to the small ribosomal subunit.
Marco Kaiser, Jan Philip Wurm, Birgit Märtens, Udo Bläsi, Denys Pogoryelov, Jens Wöhnert   +5 more
doaj   +1 more source

Acquisition of elemental sulfur by sulfur‐oxidising Sulfolobales

Environmental Microbiology, Volume 26, Issue 9, September 2024.
The distribution of sulfur oxidoreductase (SOR) homologues is patchy among Sulfolobales. Herein, physiological and ecological consequences of encoding SOR were investigated. While non‐SOR‐encoding strains required direct contact with S80 for oxidation, SOR‐encoding strains oxidised it without direct contact via hydrogen sulfide (H2S)‐promoted formation
Maria C. Fernandes‐Martins, Carli Springer, Daniel R. Colman, Eric S. Boyd   +3 more
wiley   +1 more source

Quantifying Resilience in Single-Host/Single-Virus Infections

Applied Microbiology
Due to theoretical and practical applications in biomedical, environmental, and industrial microbiology, robust metrics for quantifying the virulence of pathogens is vital.
Socheata Hour, Andrew Pierce, Sobroney Ying Heng, Ruth Plymale, Ruben Michael Ceballos   +4 more
doaj   +1 more source

Tetraether archaeal lipids promote long‐term survival in extreme conditions

Molecular Microbiology, Volume 121, Issue 5, Page 882-894, May 2024.
Unique archaeal lipid membrane composition, including membrane‐spanning, tetraether lipids, presumably contribute to survival in extreme pH, temperature, and salinity. The biosynthesis of tetraether lipids in the model species Thermococcus kodakarensis promotes survival at high temperatures, and we demonstrate that alternative lipid species are ...
Geraldy Lie Stefanus Liman, Andy A. Garcia, Kristin A. Fluke, Hayden R. Anderson, Sarah C. Davidson, Paula V. Welander, Thomas J. Santangelo   +6 more
wiley   +1 more source

Data_Sheet_2_Enzymes Catalyzing Crotonyl-CoA Conversion to Acetoacetyl-CoA During the Autotrophic CO2 Fixation in Metallosphaera sedula.xlsx

, 2020
Autotrophic Crenarchaeota use two different cycles for carbon dioxide fixation. Members of the Sulfolobales use the 3-hydroxypropionate/4-hydroxybutyrate (HP/HB) cycle, whereas Desulfurococcales and Thermoproteales use the dicarboxylate/4-hydroxybutyrate
Ivan A. Berg (8558781), Harald Huber (211414), Li Liu (75607)   +2 more
core   +1 more source

Distribution of Complex and Core Lipids within New Hyperthermophilic Members of the Archaea Domain [PDF]

, 1992
Core and complex lipids of several new hyperthermophilic archaeal isolates were analyzed. The organisms belong to the Sulfolobales,Archaeoglobus, Pyrobaculum, and Methanococcus. A detailed structural investigation of complex lipids of Pyrobaculum species
Huber, Robert, Stetter, Karl Otto, de Rosa, M., Nicolaus, B., Huber, Gertrud, Gambacorta, A., Trincone, A., Palmieri, G.   +7 more
core   +1 more source

Rational design of unrestricted pRN1 derivatives and their application in the construction of a dual plasmid vector system for Saccharolobus islandicus

mLife, Volume 3, Issue 1, Page 119-128, March 2024.
Abstract Saccharolobus islandicus REY15A represents one of the very few archaeal models with versatile genetic tools, which include efficient genome editing, gene silencing, and robust protein expression systems. However, plasmid vectors constructed for this crenarchaeon thus far are based solely on the pRN2 cryptic plasmid.
Pengpeng Zhao, Xiaonan Bi, Xiaoning Wang, Xu Feng, Yulong Shen, Guanhua Yuan, Qunxin She   +6 more
wiley   +1 more source

A novel RHH family transcription factor aCcr1 and its viral homologs dictate cell cycle progression in archaea

, 2023
International audienceCell cycle regulation is of paramount importance for all forms of life. Here, we report that a conserved and essential cell cycle-specific transcription factor (designated as aCcr1) and its viral homologs control cell division in ...
Zhang, Shuo, Yang, Yunfeng, Zhou, Fan, She, Qunxin, Fu, Xiaofei, Ni, Jinfeng, Krupovic, Mart, Zhang, Xuemei, Liu, Junfeng, Shen, Yulong, Huang, Qihong   +10 more
core   +1 more source