Results 1 to 10 of about 8,768 (160)

Archaeal GPN-loop GTPases involve a lock-switch-rock mechanism for GTP hydrolysis

mBio, 2023
Three GPN-loop GTPases, GPN1–GPN3, are central to the maturation and trafficking of eukaryotic RNA polymerase II. This GTPase family is widely represented in archaea but typically occurs as single paralogs.
Lukas Korf, Xing Ye, Marian S. Vogt, Wieland Steinchen, Mohamed Watad, Chris van der Does, Maxime Tourte, Shamphavi Sivabalasarma, Sonja-Verena Albers, Lars-Oliver Essen   +9 more
doaj   +1 more source

First Isolation and Structure Elucidation of GDNT‐β‐Glu – Tetraether Lipid Fragment from Archaeal Sulfolobus Strains

ChemistryOpen, 2021
Due to their special chemical structure, tetraether lipids (TEL) represent essential elements of archaeal membranes, providing these organisms with extraordinary properties.
Dr. Alexander Scholte, Christoph Hübner, Dr. Dieter Ströhl, Olaf Scheufler, Dr. Steffen Czich, Julia M. Börke, Dr. Gerhard Hildebrand, Prof. Dr. Klaus Liefeith   +7 more
doaj   +1 more source

Chromosome conformation capture assay combined with biotin enrichment for hyperthermophilic archaea

STAR Protocols, 2021
Summary: Chromosome organization in archaea has long been enigmatic due, in part, to the typically small cell size of archaea and the extremophilic nature of many of the model archaeal species studies, rendering live-cell imaging technically challenging.
Naomichi Takemata, Stephen D. Bell
doaj   +1 more source

Expression, purification, and characterization of a membrane-associated cyclic oligo-adenylate degrader from Sulfolobus islandicus

STAR Protocols, 2021
Summary: Type III CRISPR-cas systems initiate cyclic oligo-adenylate (cOA) signaling to initiate immune response. Previously, we identified that a membrane-associated DHH-DHHA1 family protein from Sulfolobus islandicus efficiently degrades cOA.
Ruiliang Zhao, Yang Yang, Ke Yang, Wenyuan Han   +3 more
doaj   +1 more source

High-resolution analysis of chromosome conformation in hyperthermophilic archaea

STAR Protocols, 2021
Summary: Chromosome conformation capture (3C) techniques are emerging as promising approaches to study genome organization in Archaea, the least understood domain of life in terms of chromosome biology. Here, we describe a 3C technique combined with deep
Naomichi Takemata, Stephen D. Bell
doaj   +1 more source

Effect of the Availability of the Source of Nitrogen and Phosphorus in the Bio-Oxidation of H₂S by Sulfolobus metallicus

Fermentation, 2023
The effect of nitrogen and phosphorus availability on the growth of Sulfolobus metallicus was analyzed. This archaeon was subjected to a series of nitrogen and phosphorus limitation conditions to determine their effects on growth.
Javier Silva, Rodrigo Ortiz-Soto, Marjorie Morales, Germán Aroca   +3 more
doaj   +1 more source

Species-Specific Recognition of Sulfolobales Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns

mBio, 2020
The UV-inducible pili system of Sulfolobales (Ups) mediates the formation of species-specific cellular aggregates. Within these aggregates, cells exchange DNA to repair DNA double-strand breaks via homologous recombination. Substitution of the Sulfolobus
Marleen van Wolferen, Asif Shajahan, Kristina Heinrich, Susanne Brenzinger, Ian M. Black, Alexander Wagner, Ariane Briegel, Parastoo Azadi, Sonja-Verena Albers   +8 more
doaj   +1 more source

Biochemical characterization of a structure-specific resolving enzyme from Sulfolobus islandicus rod-shaped virus 2. [PDF]

PLoS ONE, 2011
Sulfolobus islandicus rod shaped virus 2 (SIRV2) infects the archaeon Sulfolobus islandicus at extreme temperature (70°C-80°C) and acidity (pH 3). SIRV2 encodes a Holliday junction resolving enzyme (SIRV2 Hjr) that has been proposed as a key enzyme in ...
Andrew F Gardner, Chudi Guan, William E Jack   +2 more
doaj   +1 more source

Metabolism of Pentose Sugars in the Hyperthermophilic Archaea Sulfolobus solfataricus and Sulfolobus acidocaldarius [PDF]

Journal of Biological Chemistry, 2010
We have previously shown that the hyperthermophilic archaeon, Sulfolobus solfataricus, catabolizes d-glucose and d-galactose to pyruvate and glyceraldehyde via a non-phosphorylative version of the Entner-Doudoroff pathway. At each step, one enzyme is active with both C6 epimers, leading to a metabolically promiscuous pathway.
Charlotte E M, Nunn, Ulrike, Johnsen, Peter, Schönheit, Tobias, Fuhrer, Uwe, Sauer, David W, Hough, Michael J, Danson   +6 more
openaire   +2 more sources

Differential Virus Host-Ranges of the Fuselloviridae of Hyperthermophilic Archaea: Implications for Evolution in Extreme Environments

Frontiers in Microbiology, 2012
An emerging model for investigating virus-host interactions in hyperthermophilic Archaea is the Fusellovirus-Sulfolobus system. The host, Sulfolobus, is a hyperthermophilic acidophile endemic to sulfuric volcanic-driven hot springs worldwide.
Ruben Michael eCeballos, Caleb D Marceau, Joshua Ovila Marceau, Steven eMorris, Adam J Clore, Kenneth M Stedman   +5 more
doaj   +1 more source