Results 21 to 30 of about 28,271 (292)

Chromosome conformation capture assay combined with biotin enrichment for hyperthermophilic archaea

open access: yesSTAR Protocols, 2021
Summary: Chromosome organization in archaea has long been enigmatic due, in part, to the typically small cell size of archaea and the extremophilic nature of many of the model archaeal species studies, rendering live-cell imaging technically challenging.
Naomichi Takemata, Stephen D. Bell
doaj   +1 more source

Expression, purification, and characterization of a membrane-associated cyclic oligo-adenylate degrader from Sulfolobus islandicus

open access: yesSTAR Protocols, 2021
Summary: Type III CRISPR-cas systems initiate cyclic oligo-adenylate (cOA) signaling to initiate immune response. Previously, we identified that a membrane-associated DHH-DHHA1 family protein from Sulfolobus islandicus efficiently degrades cOA.
Ruiliang Zhao   +3 more
doaj   +1 more source

High-resolution analysis of chromosome conformation in hyperthermophilic archaea

open access: yesSTAR Protocols, 2021
Summary: Chromosome conformation capture (3C) techniques are emerging as promising approaches to study genome organization in Archaea, the least understood domain of life in terms of chromosome biology. Here, we describe a 3C technique combined with deep
Naomichi Takemata, Stephen D. Bell
doaj   +1 more source

The Cell Membrane of Sulfolobus spp.—Homeoviscous Adaption and Biotechnological Applications

open access: yesInternational Journal of Molecular Sciences, 2020
The microbial cell membrane is affected by physicochemical parameters, such as temperature and pH, but also by the specific growth rate of the host organism.
Kerstin Rastädter   +3 more
semanticscholar   +1 more source

Effect of the Availability of the Source of Nitrogen and Phosphorus in the Bio-Oxidation of H2S by Sulfolobus metallicus

open access: yesFermentation, 2023
The effect of nitrogen and phosphorus availability on the growth of Sulfolobus metallicus was analyzed. This archaeon was subjected to a series of nitrogen and phosphorus limitation conditions to determine their effects on growth.
Javier Silva   +3 more
doaj   +1 more source

Distinct Patterns of Antibiotic Sensitivities in Ammonia-Oxidising Archaea. [PDF]

open access: yesEnviron Microbiol
Antibiotic sensitivity varies between different strains of ammonia‐oxidising archaea. The results of this study will be useful for selective enrichment and development of a genetic system for ammonia‐oxidising archaea. ABSTRACT Ammonia‐oxidising archaea (AOA) are important microorganisms contributing towards the nitrogen flux in the environment. Unlike
Klein T   +6 more
europepmc   +2 more sources

A Spotlight on Archaea in Humans, Livestock and Poultry: A Review. [PDF]

open access: yesVet Med Sci
The microbiota includes prokaryotes (archaea and bacteria) and eukaryotes. Archaea are single‐celled prokaryotes and essential part of gut microbiome. Researches on archaea in ruminants and humans are more than mono‐gastric. The low abundance of archaea in the gut depends on the method used (metagenomics or meta‐transcriptomic) and age of people or ...
Salahi A, Abd El-Ghany WA.
europepmc   +2 more sources

Sulfuracidifex tepidarius gen. nov., sp. nov. and transfer of Sulfolobus metallicus Huber and Stetter 1992 to the genus Sulfuracidifex as Sulfuracidifex metallicus comb. nov.

open access: yesInternational Journal of Systematic and Evolutionary Microbiology, 2020
Two novel, strictly aerobic, sulfur-dependent, thermoacidophilic strains, IC-006T and IC-007, were isolated from a solfataric field at Hakone Ohwaku-dani, Kanagawa, Japan. Cells of the two strains were irregular cocci with a diameter of 1.0-1.8 µm.
T. Itoh   +5 more
semanticscholar   +1 more source

Species-Specific Recognition of Sulfolobales Mediated by UV-Inducible Pili and S-Layer Glycosylation Patterns

open access: yesmBio, 2020
The UV-inducible pili system of Sulfolobales (Ups) mediates the formation of species-specific cellular aggregates. Within these aggregates, cells exchange DNA to repair DNA double-strand breaks via homologous recombination. Substitution of the Sulfolobus
Marleen van Wolferen   +8 more
doaj   +1 more source

Biochemical characterization of a structure-specific resolving enzyme from Sulfolobus islandicus rod-shaped virus 2. [PDF]

open access: yesPLoS ONE, 2011
Sulfolobus islandicus rod shaped virus 2 (SIRV2) infects the archaeon Sulfolobus islandicus at extreme temperature (70°C-80°C) and acidity (pH 3). SIRV2 encodes a Holliday junction resolving enzyme (SIRV2 Hjr) that has been proposed as a key enzyme in ...
Andrew F Gardner   +2 more
doaj   +1 more source

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