Results 21 to 30 of about 16,065 (253)

5'-untranslated region sequences enhance plasmid-based protein production in Sulfolobus acidocaldarius. [PDF]

Front Microbiol
Kuschmierz L, Wagner A, Schmerling C, Busche T, Kalinowski J, Bräsen C, Siebers B.   +6 more
europepmc   +3 more sources

The Lrs14-Like AbfR1 Homolog From Metallosphaera sedula Is a Nucleoid-Organizing Protein. [PDF]

Microbiologyopen
Nucleoid organization in Crenarchaeota involves diverse small DNA‐binding proteins. The Lrs14‐type protein AbfR1 from Metallosphaera sedula binds nonsequence specifically across the genome and induces DNA condensation. These findings suggest a structural role for AbfR1Ms in chromatin architecture, functionally resembling bacterial nucleoid‐associated ...
De Kock V, Willaert R, Gansemans Y, Van Nieuwerburgh F, Baes R, Peeters E.   +5 more
europepmc   +2 more sources

Synthesis and Incorporation of a pH-Responsive Nucleoside Into DNA Sequences. [PDF]

Chembiochem
A pH‐responsive nucleoside is developed that enables a reversible switch in DNA between paired and unpaired states near physiological pH. This design may provide the framework for sequence‐independent, pH programable DNA motifs, thus paving the way for dynamic DNA nanotechnology applications.
Ogel E, Becker S.
europepmc   +2 more sources

First Isolation and Structure Elucidation of GDNT‐β‐Glu – Tetraether Lipid Fragment from Archaeal Sulfolobus Strains

ChemistryOpen, 2021
Due to their special chemical structure, tetraether lipids (TEL) represent essential elements of archaeal membranes, providing these organisms with extraordinary properties.
Dr. Alexander Scholte, Christoph Hübner, Dr. Dieter Ströhl, Olaf Scheufler, Dr. Steffen Czich, Julia M. Börke, Dr. Gerhard Hildebrand, Prof. Dr. Klaus Liefeith   +7 more
doaj   +1 more source

Groundwater Microbial Diversity Associated With Icelandic Basaltic Subsurface Environments. [PDF]

Environ Microbiol Rep
Overview of microbial diversity and environmental drivers in Icelandic deep basaltic aquifers. Using 16S rRNA gene metabarcoding across 22 geothermal wells spanning broad temperature, pH, and bedrock‐age gradients, the study reveals distinct bacterial and archaeal community structures shaped by temperature, pH, and host‐rock reactivity.
Bas-Lorillot J, Ménez B, Wild B, Borrel G, Le Bihan M, Stefánsson A, Gunnarsson-Robin J, Bjarkadóttir AB, Aðalsteinsdóttir SM, Tisserand D, Daval D, Gérard E.   +11 more
europepmc   +2 more sources

Chromosome conformation capture assay combined with biotin enrichment for hyperthermophilic archaea

STAR Protocols, 2021
Summary: Chromosome organization in archaea has long been enigmatic due, in part, to the typically small cell size of archaea and the extremophilic nature of many of the model archaeal species studies, rendering live-cell imaging technically challenging.
Naomichi Takemata, Stephen D. Bell
doaj   +1 more source

Expression, purification, and characterization of a membrane-associated cyclic oligo-adenylate degrader from Sulfolobus islandicus

STAR Protocols, 2021
Summary: Type III CRISPR-cas systems initiate cyclic oligo-adenylate (cOA) signaling to initiate immune response. Previously, we identified that a membrane-associated DHH-DHHA1 family protein from Sulfolobus islandicus efficiently degrades cOA.
Ruiliang Zhao, Yang Yang, Ke Yang, Wenyuan Han   +3 more
doaj   +1 more source

The influence of the specific growth rate on the lipid composition of Sulfolobus acidocaldarius

Extremophiles, 2020
Archaeal lipids are constituted of two isoprenoid chains connected via ether bonds to glycerol in the sn-2, 3 position. Due to these unique properties archaeal lipids are significantly more stable against high temperature, low pH, oxidation and enzymatic
Julian Quehenberger, E. Pittenauer, G. Allmaier, O. Spadiut   +3 more
semanticscholar   +1 more source

High-resolution analysis of chromosome conformation in hyperthermophilic archaea

STAR Protocols, 2021
Summary: Chromosome conformation capture (3C) techniques are emerging as promising approaches to study genome organization in Archaea, the least understood domain of life in terms of chromosome biology. Here, we describe a 3C technique combined with deep
Naomichi Takemata, Stephen D. Bell
doaj   +1 more source

The Cell Membrane of Sulfolobus spp.—Homeoviscous Adaption and Biotechnological Applications

International Journal of Molecular Sciences, 2020
The microbial cell membrane is affected by physicochemical parameters, such as temperature and pH, but also by the specific growth rate of the host organism.
Kerstin Rastädter, D. Wurm, O. Spadiut, Julian Quehenberger   +3 more
semanticscholar   +1 more source