Application of the endogenous CRISPR-Cas type I-D system for genetic engineering in the thermoacidophilic archaeon Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2023 CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems are widely distributed among bacteria and archaea. In this study, we demonstrate the successful utilization of the type I-D CRISPR-Cas system for genetic engineering in the ...
Jan Bost +5 more
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Exopolysaccharide composition and size in Sulfolobus acidocaldarius biofilms [PDF]
Frontiers in Microbiology, 2022 Extracellular polymeric substances (EPS) comprise mainly carbohydrates, proteins and extracellular DNA (eDNA) in biofilms formed by the thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius. However, detailed information on the carbohydrates in the S.
Laura Kuschmierz +7 more
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Translesion synthesis of apurinic/apyrimidic siteanalogues by Y-family DNA polymerase Dbh fromSulfolobus acidocaldarius [PDF]
Acta Biochimica et Biophysica Sinica, 2022 Apurinic/apyrimidic (AP) sites are severe DNA damages and strongly block DNA extension by major DNA polymerases. Y-family DNA polymerases possess a strong ability to bypass AP sites and continue the DNA synthesis reaction, which is called translesion ...
Wang Weiwei +7 more
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Identification of XylR, the Activator of Arabinose/Xylose Inducible Regulon in Sulfolobus acidocaldarius and Its Application for Homologous Protein Expression [PDF]
Frontiers in Microbiology, 2020 The thermophilic archaeon Sulfolobus acidocaldarius can use different carbon sources for growth, including the pentoses D-xylose and L-arabinose. In this study, we identified the activator XylR (saci_2116) responsible for the transcriptional regulation ...
Nienke van der Kolk +7 more
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High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2021 Significant technical challenges have limited the study of extremophile cell biology. Here we describe a system for imaging samples at 75°C using high numerical aperture, oil-immersion lenses.
Arthur Charles-Orszag +2 more
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Effects of genomic location on ectopic integration and gene expression of a reporter gene cassette in Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologyIn eukaryotes and bacteria, it is well-established that the genomic location of ectopic gene integration influences the expression level due to replication-associated gene dosage effects as well as effects mediated by chromatin organization. In contrast,
Yifei Xu +5 more
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Machine Learning Uncovers a Data-Driven Transcriptional Regulatory Network for the Crenarchaeal Thermoacidophile Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2021 Dynamic cellular responses to environmental constraints are coordinated by the transcriptional regulatory network (TRN), which modulates gene expression.
Siddharth M. Chauhan +9 more
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Impact of nutrient excess on physiology and metabolism of Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologyOverflow metabolism is a well-known phenomenon that describes the seemingly wasteful and incomplete substrate oxidation by aerobic cells, such as yeasts, bacteria, and mammalian cells, even when conditions allow for total combustion via respiration. This
Viktor Laurin Sedlmayr +8 more
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DNA-Binding Properties of a Novel Crenarchaeal Chromatin-Organizing Protein in Sulfolobus acidocaldarius [PDF]
Biomolecules, 2022 In archaeal microorganisms, the compaction and organization of the chromosome into a dynamic but condensed structure is mediated by diverse chromatin-organizing proteins in a lineage-specific manner. While many archaea employ eukaryotic-type histones for
Liesbeth Lemmens +7 more
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5′-untranslated region sequences enhance plasmid-based protein production in Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologySulfolobus acidocaldarius, a thermoacidophilic archaeon of the phylum Thermoproteota (former Crenarchaeota), is a widely used model organism for gene deletion studies and recombinant protein production.
Laura Kuschmierz +6 more
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