An unusual glycerol-3-phosphate dehydrogenase in Sulfolobus acidocaldarius elucidates the diversity of glycerol metabolism across Archaea [PDF]
Communications BiologyGlycerol is highly abundant in natural ecosystems and serves as both an important carbon source for microorganisms as well as a promising feedstock for industrial applications.
Christian Schmerling +14 more
doaj +3 more sources
A TetR-family transcription factor regulates fatty acid metabolism in the archaeal model organism Sulfolobus acidocaldarius [PDF]
Nature Communications, 2019 Certain archaea appear to metabolize fatty acids, but the regulation of these pathways is unclear. Here, Wang et al. provide genetic, functional and structural evidence supporting that a TetR-family transcriptional regulator is involved in regulation of ...
David Sybers +2 more
exaly +3 more sources
5′-untranslated region sequences enhance plasmid-based protein production in Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologySulfolobus acidocaldarius, a thermoacidophilic archaeon of the phylum Thermoproteota (former Crenarchaeota), is a widely used model organism for gene deletion studies and recombinant protein production.
Laura Kuschmierz +6 more
doaj +4 more sources
Exopolysaccharide composition and size in Sulfolobus acidocaldarius biofilms [PDF]
Frontiers in Microbiology, 2022 Extracellular polymeric substances (EPS) comprise mainly carbohydrates, proteins and extracellular DNA (eDNA) in biofilms formed by the thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius. However, detailed information on the carbohydrates in the S.
Laura Kuschmierz +7 more
doaj +2 more sources
Application of the endogenous CRISPR-Cas type I-D system for genetic engineering in the thermoacidophilic archaeon Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2023 CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems are widely distributed among bacteria and archaea. In this study, we demonstrate the successful utilization of the type I-D CRISPR-Cas system for genetic engineering in the ...
Jan Bost +5 more
doaj +2 more sources
Translesion synthesis of apurinic/apyrimidic siteanalogues by Y-family DNA polymerase Dbh fromSulfolobus acidocaldarius [PDF]
Acta Biochimica et Biophysica Sinica, 2022 Apurinic/apyrimidic (AP) sites are severe DNA damages and strongly block DNA extension by major DNA polymerases. Y-family DNA polymerases possess a strong ability to bypass AP sites and continue the DNA synthesis reaction, which is called translesion ...
Wang Weiwei +7 more
doaj +2 more sources
Identification of XylR, the Activator of Arabinose/Xylose Inducible Regulon in Sulfolobus acidocaldarius and Its Application for Homologous Protein Expression [PDF]
Frontiers in Microbiology, 2020 The thermophilic archaeon Sulfolobus acidocaldarius can use different carbon sources for growth, including the pentoses D-xylose and L-arabinose. In this study, we identified the activator XylR (saci_2116) responsible for the transcriptional regulation ...
Nienke van der Kolk +7 more
doaj +2 more sources
High-Temperature Live-Cell Imaging of Cytokinesis, Cell Motility, and Cell-Cell Interactions in the Thermoacidophilic Crenarchaeon Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2021 Significant technical challenges have limited the study of extremophile cell biology. Here we describe a system for imaging samples at 75°C using high numerical aperture, oil-immersion lenses.
Arthur Charles-Orszag +2 more
doaj +2 more sources
Effects of genomic location on ectopic integration and gene expression of a reporter gene cassette in Sulfolobus acidocaldarius [PDF]
Frontiers in MicrobiologyIn eukaryotes and bacteria, it is well-established that the genomic location of ectopic gene integration influences the expression level due to replication-associated gene dosage effects as well as effects mediated by chromatin organization. In contrast,
Yifei Xu +5 more
doaj +2 more sources
Machine Learning Uncovers a Data-Driven Transcriptional Regulatory Network for the Crenarchaeal Thermoacidophile Sulfolobus acidocaldarius [PDF]
Frontiers in Microbiology, 2021 Dynamic cellular responses to environmental constraints are coordinated by the transcriptional regulatory network (TRN), which modulates gene expression.
Siddharth M. Chauhan +9 more
doaj +2 more sources