Control of cyclic oligoadenylate synthesis in a type III CRISPR system
eLife, 2018 The CRISPR system for prokaryotic adaptive immunity provides RNA-mediated protection from viruses and mobile genetic elements. When viral RNA transcripts are detected, type III systems adopt an activated state that licenses DNA interference and synthesis
Christophe Rouillon +4 more
doaj +1 more source
Genes and Environment, 2022 Urea (Ua) is produced in DNA as the result of oxidative damage to thymine and guanine. It was previously reported that Klenow fragment (Kf) exo− incorporated dATP opposite Ua, and that DNA polymerase β was blocked by Ua.
Taishu Kawada +8 more
doaj +1 more source
ChemBioChem, Volume 26, Issue 11, June 3, 2025.An affitin derived from the Sac7d scaffold enables the light‐controlled affinity purification of antibodies under physiological conditions, thus overcoming limitations of protein A chromatography. X‐ray crystallographic analysis and affinity measurements reveal complex formation with Fc at the CH2/CH3 junction, similar to protein A/G and FnRn. Combined
Felix Veitl +7 more
wiley +1 more source
Hyperthermophilic endoglucanase for
Biotechnology for Biofuels, 2012 Background The enzymatic conversion of lignocellulosic plant biomass into fermentable sugars is a crucial step in the sustainable and environmentally friendly production of biofuels.
Klose Holger +4 more
doaj +1 more source
Revisit to the biodesulfurization capability of hyperthermophilic archeon Sulfolobus Solfataricus P2 revealed DBT consumption by the organism in an oil/water two-phase liquid system at high temperatures [PDF]
, 2014 The ability of hyperthermophilic archaeon, Sulfolobus solfataricus P2, to grow on organic and inorganic sulfur sources was investigated. A sulfur free mineral medium has been employed with different sources of carbon.
Dinler Doğanay, Gizem +2 more
core
Mutational analysis of an archaeal minichromosome maintenance protein exterior hairpin reveals critical residues for helicase activity and DNA binding [PDF]
, 2010 Background The mini-chromosome maintenance protein (MCM) complex is an essential replicative helicase for DNA replication in Archaea and Eukaryotes.
Aaron S Brewster +3 more
core +1 more source
A Spotlight on Archaea in Humans, Livestock and Poultry: A Review
Veterinary Medicine and Science, Volume 11, Issue 2, March 2025.The microbiota includes prokaryotes (archaea and bacteria) and eukaryotes. Archaea are single‐celled prokaryotes and essential part of gut microbiome. Researches on archaea in ruminants and humans are more than mono‐gastric. The low abundance of archaea in the gut depends on the method used (metagenomics or meta‐transcriptomic) and age of people or ...
Ahmad Salahi, Wafaa A. Abd El‐Ghany
wiley +1 more source
Inducible and constitutive promoters for genetic systems in Sulfolobus acidocaldarius [PDF]
, 2018 Central to genetic work in any organism are the availability of a range of inducible and constitutive promoters. In this work we studied several promoters for use in the hyperthermophilic archaeon Sulfolobus acidocaldarius. The promoters were tested with
Albers, Sonja-Verena +3 more
core
Distinct Patterns of Antibiotic Sensitivities in Ammonia‐Oxidising Archaea
Environmental Microbiology, Volume 27, Issue 3, March 2025.Antibiotic sensitivity varies between different strains of ammonia‐oxidising archaea. The results of this study will be useful for selective enrichment and development of a genetic system for ammonia‐oxidising archaea. ABSTRACT Ammonia‐oxidising archaea (AOA) are important microorganisms contributing towards the nitrogen flux in the environment. Unlike
Timothy Klein +6 more
wiley +1 more source
hSSB1 interacts directly with the MRN complex stimulating its recruitment to DNA double-strand breaks and its endo-nuclease activity [PDF]
, 2013 hSSB1 is a recently discovered single-stranded DNA binding protein that is essential for efficient repair of DNA double-strand breaks (DSBs) by the homologous recombination pathway.
Bain, Amanda +7 more
core +1 more source