Results 101 to 110 of about 10,749 (205)

Multiple nucleic acid cleavage modes in divergent type III CRISPR systems [PDF]

open access: yes, 2016
CRISPR-Cas is an RNA-guided adaptive immune system that protects bacteria and archaea from invading nucleic acids. Type III systems (Cmr, Csm) have been shown to cleave RNA targets in vitro and some are capable of transcription-dependent DNA targeting ...
Zhang, Jing   +6 more
core   +1 more source

Amidst multiple binding orientations on fork DNA, Saccharolobus MCM helicase proceeds N-first for unwinding

open access: yeseLife, 2019
DNA replication requires that the duplex genomic DNA strands be separated; a function that is implemented by ring-shaped hexameric helicases in all Domains.
Himasha M Perera, Michael A Trakselis
doaj   +1 more source

Structural analysis of the Sulfolobus solfataricus TF55β chaperonin by cryo-electron microscopy. [PDF]

open access: yesActa Crystallogr F Struct Biol Commun, 2021
Zeng YC, Sobti M, Stewart AG.
europepmc   +1 more source

Gelrite plate technique for culturing Sulfolobus solfataricus, a thermoacidophilic archaebacterium

open access: yes, 1991
A rapid procedure for plating the thermo-acidophilic archaebacterium Sulfolobus solfataricus using Gerlite as a gelling agent is reported. The technique uses a clear, single layer in which the colonies are grown.
Romano I   +4 more
core   +1 more source

CRISPR RNA biogenesis by a Cas6 nuclease [PDF]

open access: yes, 2015
Clustered regularly interspaced short palindromic repeats (CRISPRs) and associated (Cas) proteins form the basis of a prokaryotic adaptive immune system.
Sokolowski, Richard D.
core   +1 more source

Differential active site loop conformations mediate promiscuous activities in the lactonase SsoPox.

open access: yesPLoS ONE, 2013
Enzymes are proficient catalysts that enable fast rates of Michaelis-complex formation, the chemical step and products release. These different steps may require different conformational states of the active site that have distinct binding properties ...
Julien Hiblot   +3 more
doaj   +1 more source

Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-) archaea [PDF]

open access: yes, 2007
The extreme thermoacidophiles of the genus Sulfolobus are among the best-studied archaea but have lacked small, reliable plasmid vectors, which have proven extremely useful for manipulating and analyzing genes in other microorganisms.
Berkner, Silvia   +6 more
core   +1 more source

Home - About - Disclaimer - Privacy