An Autonomously Replicating Transforming Vector for Sulfolobus solfataricus [PDF]
ABSTRACT A plasmid able to transform and to be stably maintained both in Sulfolobus solfataricus and in Escherichia coli was constructed by insertion into an E. coli plasmid of the autonomously replicating sequence of the virus particle SSV1 and a suitable ...
R. Cannio +3 more
openaire +5 more sources
Nucleases: From Primitive Immune Defenders to Modern Biotechnology Tools
The evolution of nucleases is a remarkable journey, starting as primitive bacterial defenders against bacteriophages. Over time, nucleases have adapted to specialised roles within the immune systems of all forms of life, culminating in humans, where DNases and RNases serve as key components of the immune system.
Frank J. Hernandez
wiley +1 more source
N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier [PDF]
N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in ...
Abu-Qarn +38 more
core +3 more sources
Identification, characterization and classification of prokaryotic nucleoid‐associated proteins
Nucleoid‐associated proteins (NAPs) structure DNA by either bending, wrapping, bridging, or forming protein filaments on the DNA. We review newly identified NAPs and provide a list of simple biochemical assays to study the DNA‐structuring properties of novel NAPs. Abstract Common throughout life is the need to compact and organize the genome.
Samuel Schwab, Remus T. Dame
wiley +1 more source
Chromatin and gene regulation in archaea
Recent data from diverse archaea with different repertoires of nucleoid‐associated proteins show a common genome‐wide chromatin organisation where chromatin maintains access to gene promoters independently of transcription activity. Abstract The chromatinisation of DNA by nucleoid‐associated proteins (NAPs) in archaea ‘formats’ the genome structure in ...
Fabian Blombach, Finn Werner
wiley +1 more source
Novel Archaeal DNA Polymerase B from Domas Hot Spring West Java [PDF]
Nine novel archaeal DNA polymerase genes from Domas Hot Spring, West Java have been cloned directly through the natural sample.
Akhmaloka, A. +4 more
core
Improving the 'tool box' for robust industrial enzymes. [PDF]
This is the final version of the article. Available from Springer Verlag via the DOI in this record.The speed of sequencing of microbial genomes and metagenomes is providing an ever increasing resource for the identification of new robust biocatalysts ...
Littlechild, JA
core +1 more source
Regulation of DNA Topology in Archaea: State of the Art and Perspectives
DNA topoisomerases are ubiquitous enzymes that play a crucial role in regulating DNA supercoiling which affects fundamental biological processes involving DNA. In this review, we summarize the current understanding of regulation of supercoiling by DNA topoisomerases in the third domain of life, the Archaea, with a particular focus on three key model ...
Paul Villain, Tamara Basta
wiley +1 more source
Regulatory Properties of Glutamate Dehydrogenase from Sulfolobus solfataricus
The purified glutamate dehydrogenase (GDH) from Sulfolobus solfataricus showed remarkable thermostability and retained 90-95% of the initial activity after incubation at -20 degrees C, 4 degrees C, and 25 degrees C for up to 6 months. Unlike mammalian GDHs, the activity of GDH from Sulfolobus solfataricus was not significantly affected by the presence ...
J Y, Ahn, K S, Lee, S Y, Choi, S W, Cho
openaire +2 more sources
Identification of novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A
Abstract In Sulfolobales cells, transcription of the Ups (UV‐inducible pili of Sulfolobus) and Ced (Crenarchaeal system for exchange of DNA) genes is highly induced by DNA damage, and the two systems play key roles in pili‐mediated cell aggregation and chromosomal DNA import, respectively.
Pengju Wu +6 more
wiley +1 more source

