Results 101 to 110 of about 11,764 (212)

An Autonomously Replicating Transforming Vector for Sulfolobus solfataricus [PDF]

open access: yesJournal of Bacteriology, 1998
ABSTRACT A plasmid able to transform and to be stably maintained both in Sulfolobus solfataricus and in Escherichia coli was constructed by insertion into an E. coli plasmid of the autonomously replicating sequence of the virus particle SSV1 and a suitable ...
R. Cannio   +3 more
openaire   +5 more sources

Nucleases: From Primitive Immune Defenders to Modern Biotechnology Tools

open access: yesImmunology, Volume 174, Issue 3, Page 279-286, March 2025.
The evolution of nucleases is a remarkable journey, starting as primitive bacterial defenders against bacteriophages. Over time, nucleases have adapted to specialised roles within the immune systems of all forms of life, culminating in humans, where DNases and RNases serve as key components of the immune system.
Frank J. Hernandez
wiley   +1 more source

N-glycosylation in the thermoacidophilic archaeon Sulfolobus acidocaldarius involves a short dolichol pyrophosphate carrier [PDF]

open access: yes, 2016
N-glycosylation is a post-translational modification that occurs across evolution. In the thermoacidophilic archaea Sulfolobus acidocaldarius, glycoproteins are modified by an N-linked tribranched hexasaccharide reminiscent of the N-glycans assembled in ...
Abu-Qarn   +38 more
core   +3 more sources

Identification, characterization and classification of prokaryotic nucleoid‐associated proteins

open access: yesMolecular Microbiology, Volume 123, Issue 3, Page 206-217, March 2025.
Nucleoid‐associated proteins (NAPs) structure DNA by either bending, wrapping, bridging, or forming protein filaments on the DNA. We review newly identified NAPs and provide a list of simple biochemical assays to study the DNA‐structuring properties of novel NAPs. Abstract Common throughout life is the need to compact and organize the genome.
Samuel Schwab, Remus T. Dame
wiley   +1 more source

Chromatin and gene regulation in archaea

open access: yesMolecular Microbiology, Volume 123, Issue 3, Page 218-231, March 2025.
Recent data from diverse archaea with different repertoires of nucleoid‐associated proteins show a common genome‐wide chromatin organisation where chromatin maintains access to gene promoters independently of transcription activity. Abstract The chromatinisation of DNA by nucleoid‐associated proteins (NAPs) in archaea ‘formats’ the genome structure in ...
Fabian Blombach, Finn Werner
wiley   +1 more source

Novel Archaeal DNA Polymerase B from Domas Hot Spring West Java [PDF]

open access: yes, 2015
Nine novel archaeal DNA polymerase genes from Domas Hot Spring, West Java have been cloned directly through the natural sample.
Akhmaloka, A.   +4 more
core  

Improving the 'tool box' for robust industrial enzymes. [PDF]

open access: yes, 2017
This is the final version of the article. Available from Springer Verlag via the DOI in this record.The speed of sequencing of microbial genomes and metagenomes is providing an ever increasing resource for the identification of new robust biocatalysts ...
Littlechild, JA
core   +1 more source

Regulation of DNA Topology in Archaea: State of the Art and Perspectives

open access: yesMolecular Microbiology, Volume 123, Issue 3, Page 245-264, March 2025.
DNA topoisomerases are ubiquitous enzymes that play a crucial role in regulating DNA supercoiling which affects fundamental biological processes involving DNA. In this review, we summarize the current understanding of regulation of supercoiling by DNA topoisomerases in the third domain of life, the Archaea, with a particular focus on three key model ...
Paul Villain, Tamara Basta
wiley   +1 more source

Regulatory Properties of Glutamate Dehydrogenase from Sulfolobus solfataricus

open access: yesMolecules and Cells, 2000
The purified glutamate dehydrogenase (GDH) from Sulfolobus solfataricus showed remarkable thermostability and retained 90-95% of the initial activity after incubation at -20 degrees C, 4 degrees C, and 25 degrees C for up to 6 months. Unlike mammalian GDHs, the activity of GDH from Sulfolobus solfataricus was not significantly affected by the presence ...
J Y, Ahn, K S, Lee, S Y, Choi, S W, Cho
openaire   +2 more sources

Identification of novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A

open access: yesmLife, Volume 4, Issue 1, Page 17-28, February 2025.
Abstract In Sulfolobales cells, transcription of the Ups (UV‐inducible pili of Sulfolobus) and Ced (Crenarchaeal system for exchange of DNA) genes is highly induced by DNA damage, and the two systems play key roles in pili‐mediated cell aggregation and chromosomal DNA import, respectively.
Pengju Wu   +6 more
wiley   +1 more source

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