Results 81 to 90 of about 11,764 (212)

The dynamic interplay of host and viral enzymes in type III CRISPR-mediated cyclic nucleotide signalling

eLife, 2020
Cyclic nucleotide second messengers are increasingly implicated in prokaryotic anti-viral defence systems. Type III CRISPR systems synthesise cyclic oligoadenylate (cOA) upon detecting foreign RNA, activating ancillary nucleases that can be toxic to ...
Januka S Athukoralage, Shirley Graham, Christophe Rouillon, Sabine Grüschow, Clarissa M Czekster, Malcolm F White   +5 more
doaj   +1 more source

Rings in the Extreme: PCNA Interactions and Adaptations in the Archaea [PDF]

, 2012
Biochemical and structural analysis of archaeal proteins has enabled us to gain great insight into many eukaryotic processes, simultaneously offering fascinating glimpses into the adaptation and evolution of proteins at the extremes of life. The archaeal
Bunting, KA, Winter, JA
core   +2 more sources

The Lrs14‐Like AbfR1 Homolog From Metallosphaera sedula Is a Nucleoid‐Organizing Protein

MicrobiologyOpen, Volume 14, Issue 5, October 2025.
Nucleoid organization in Crenarchaeota involves diverse small DNA‐binding proteins. The Lrs14‐type protein AbfR1 from Metallosphaera sedula binds nonsequence specifically across the genome and induces DNA condensation. These findings suggest a structural role for AbfR1Ms in chromatin architecture, functionally resembling bacterial nucleoid‐associated ...
Veerke De Kock, Ronnie Willaert, Yannick Gansemans, Filip Van Nieuwerburgh, Rani Baes, Eveline Peeters   +5 more
wiley   +1 more source

Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.

PLoS ONE, 2013
Recent studies identified a 5´ to 3´ exoribonuclease termed Sso-RNase J in the crenarchaeon Sulfolobus solfataricus (Sso), which has been reclassified to the aCPSF2 (archaeal cleavage and polyadenylation specificity factor 2) group of β-CASP proteins. In
Birgit Märtens, Fabian Amman, Salim Manoharadas, Lukas Zeichen, Alvaro Orell, Sonja-Verena Albers, Ivo Hofacker, Udo Bläsi   +7 more
doaj   +1 more source

Structure and mechanism of the CMR complex for CRISPR-Mediated antiviral immunity [PDF]

, 2015
The prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading viruses and incorporated into ribonucleoprotein complexes with CRISPR-associated (CAS) proteins ...
Albers, Sonja-Verena, Brugger, Kim, Cannone, Giuseppe, Graham, Shirley, Kerou, Melina, Liu, Huanting, Naismith, James H., Reeks, Judith, Reimann, Julia, Rouillon, Christophe, Spagnolo, Laura, White, Malcolm F, Zhang, Jing   +12 more
core   +1 more source

Quantifying Soil Microbiome Abundance by Metatranscriptomics and Complementary Molecular Techniques—Cross‐Validation and Perspectives

Molecular Ecology Resources, Volume 25, Issue 7, October 2025.
ABSTRACT Linking meta‐omics and biogeochemistry approaches in soils has remained challenging. This study evaluates the use of an internal RNA extraction standard and its potential for making quantitative estimates of a given microbial community size (biomass) in soil metatranscriptomics. We evaluate commonly used laboratory protocols for RNA processing,
Mathilde Borg Dahl, Stella Brachmann, Andrea Söllinger, Marina Schnell, Laureen Ahlers, Magdalena Wutkowska, Katharina J. Hoff, Neetika Nath, Verena Groß, Haitao Wang, Micha Weil, Marc Piecha, Marc Schaffer, Corinna Jensen, Andreas W. Kuss, Christoph Gall, Erika Wimmer, Thomas Pribasnig, Alexander Tøsdal Tveit, Bjarni D. Sigurdsson, Christa Schleper, Andreas Richter, Tim Urich   +22 more
wiley   +1 more source

Primase-polymerases are a functionally diverse superfamily of replication and repair enzymes [PDF]

, 2015
Until relatively recently, DNA primases were viewed simply as a class of proteins that synthesize short RNA primers requisite for the initiation of DNA replication.
Aidan J. Doherty, Benjamin A. Keen, Bianchi, Bouché, Collins, Crute, Kaguni, Klinedinst, Klinedinst, Koonin, Marsden, Moss, Nigel C. Brissett, Rowen, Scherzinger, Sherman, Takechi, Thomas A. Guilliam   +17 more
core   +1 more source

Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot spring

Electronic Journal of Biotechnology, 2017
Background: Sulphur-oxidizing microorganisms are widely used in the biofiltration of total reduced sulphur compounds (odorous and neurotoxic) produced by industries such as the cellulose and petrochemical industries, which include high-temperature ...
Emky Valdebenito-Rolack, Nathaly Ruiz-Tagle, Leslie Abarzúa, Germán Aroca, Homero Urrutia   +4 more
doaj   +1 more source

Characterization, crystallization and preliminary X-ray investigation of glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus. [PDF]

, 2015
Comparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tRecombinant Sulfolobus solfataricus glyceraldehyde-3-phosphate dehydrogenase has been purified and found to be a tetramer of 148 kDa. The enzyme shows dual cofactor specificity and uses NADP+
Cowan, DA, Fleming, TM, Isupov, MN, Jones, CE, Littlechild, JA, Piper, PW   +5 more
core   +1 more source

Structure of the heterotrimeric PCNA fromSulfolobus solfataricus [PDF]

Acta Crystallographica Section F Structural Biology and Crystallization Communications, 2006
PCNA is a ring-shaped protein that encircles DNA, providing a platform for the association of a wide variety of DNA-processing enzymes that utilize the PCNA sliding clamp to maintain proximity to their DNA substrates. PCNA is a homotrimer in eukaryotes, but a heterotrimer in crenarchaea such as Sulfolobus solfataricus.
Williams, Gareth J., Johnson, Kenneth, Rudolf, Jana, McMahon, Stephen A., Carter, Lester, Oke, Muse, Liu, Huanting, Taylor, Garry L., White, Malcolm F., Naismith, James H.   +9 more
openaire   +2 more sources