Results 81 to 90 of about 7,405 (197)

The bindosome is a structural component of the Sulfolobus solfataricus cell envelope [PDF]

Extremophiles, 2011
Sugar binding proteins of the thermoacidophile Sulfolobus solfataricus function together with ABC transporters in the uptake of sugars. They are synthesized as precursors with a class III signal peptide that are normally found in archaeal flagellins and bacterial type IV pilins. The functional expression of sugar binding proteins at the cell surface is
Zolghadr, Behnam, Klingl, Andreas, Rachel, Reinard, Driessen, Arnold J. M., Albers, Sonja Verena   +4 more
openaire   +5 more sources

Control of cyclic oligoadenylate synthesis in a type III CRISPR system

eLife, 2018
The CRISPR system for prokaryotic adaptive immunity provides RNA-mediated protection from viruses and mobile genetic elements. When viral RNA transcripts are detected, type III systems adopt an activated state that licenses DNA interference and synthesis
Christophe Rouillon, Januka S Athukoralage, Shirley Graham, Sabine Grüschow, Malcolm F White   +4 more
doaj   +1 more source

Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

Genes and Environment, 2022
Urea (Ua) is produced in DNA as the result of oxidative damage to thymine and guanine. It was previously reported that Klenow fragment (Kf) exo− incorporated dATP opposite Ua, and that DNA polymerase β was blocked by Ua.
Taishu Kawada, Katsuhito Kino, Kyousuke Tokorodani, Ryuto Anabuki, Masayuki Morikawa, Takanobu Kobayashi, Kazuaki Ohara, Takayuki Ohshima, Hiroshi Miyazawa   +8 more
doaj   +1 more source

Engineering and Structural Elucidation of a Sac7d‐Derived IgG Fc‐Specific Affitin and Its Application for the Light‐Controlled Affinity Purification of Antibodies

ChemBioChem, Volume 26, Issue 11, June 3, 2025.
An affitin derived from the Sac7d scaffold enables the light‐controlled affinity purification of antibodies under physiological conditions, thus overcoming limitations of protein A chromatography. X‐ray crystallographic analysis and affinity measurements reveal complex formation with Fc at the CH2/CH3 junction, similar to protein A/G and FnRn. Combined
Felix Veitl, Andreas Eichinger, Peter Mayrhofer, Markus R. Anneser, Mauricio Testanera, Kilian Rauscher, Matthias Lenz, Arne Skerra   +7 more
wiley   +1 more source

Hot and crispy : CRISPR-Cas systems in the hyperthermophile Sulfolobus solfataricus

, 2013
The CRISPR (clustered regularly interspaced short palindromic repeats) and Cas (CRISPR-associated) genes are widely spread in bacteria and archaea, representing an intracellular defence system against invading viruses and plasmids.
Zhang, Jing, White, Malcolm F.
core   +1 more source

Hyperthermophilic endoglucanase for in planta lignocellulose conversion

Biotechnology for Biofuels, 2012
Background The enzymatic conversion of lignocellulosic plant biomass into fermentable sugars is a crucial step in the sustainable and environmentally friendly production of biofuels.
Klose Holger, Röder Juliane, Girfoglio Michele, Fischer Rainer, Commandeur Ulrich   +4 more
doaj   +1 more source

A Spotlight on Archaea in Humans, Livestock and Poultry: A Review

Veterinary Medicine and Science, Volume 11, Issue 2, March 2025.
The microbiota includes prokaryotes (archaea and bacteria) and eukaryotes. Archaea are single‐celled prokaryotes and essential part of gut microbiome. Researches on archaea in ruminants and humans are more than mono‐gastric. The low abundance of archaea in the gut depends on the method used (metagenomics or meta‐transcriptomic) and age of people or ...
Ahmad Salahi, Wafaa A. Abd El‐Ghany
wiley   +1 more source

Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach

, 2015
People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University
J. Carlos Penedo, Maria Figueira-gonzalez, White, Malcolm F, Ackermann, Katrin, Katrin Ackermann, Penedo, Carlos, Peregrina, Jose Ramon, Bode, Bela Ernest, Michael J. Morten, Figueira-Gonzalez, Maria, Malcolm F. White, Bela E. Bode, Morten, Michael John, Jose R. Peregrina   +13 more
core   +1 more source

Distinct Patterns of Antibiotic Sensitivities in Ammonia‐Oxidising Archaea

Environmental Microbiology, Volume 27, Issue 3, March 2025.
Antibiotic sensitivity varies between different strains of ammonia‐oxidising archaea. The results of this study will be useful for selective enrichment and development of a genetic system for ammonia‐oxidising archaea. ABSTRACT Ammonia‐oxidising archaea (AOA) are important microorganisms contributing towards the nitrogen flux in the environment. Unlike
Timothy Klein, Logan H. Hodgskiss, Max Dreer, J. Colin Murrell, Matthew I. Hutchings, Christa Schleper, Laura E. Lehtovirta‐Morley   +6 more
wiley   +1 more source

Protein-protein interactions leading to recruitment of the host DNA sliding clamp by the hyperthermophilic Sulfolobus islandicus rod- shaped virus 2

, 2014
Viruses infecting hyperthermophilic archaea typically do not encode DNA polymerases, raising questions regarding their genome replication. Here, using a yeast two-hybrid approach, we have assessed interactions between proteins of Sulfolobus islandicus ...
Gardner, A.F., Bell, S.D., Krupovic, M., Prangishvili, D., White, M.F.   +4 more
core   +1 more source