Results 81 to 90 of about 10,749 (205)

Identification and characterization of small RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus. [PDF]

PLoS ONE, 2012
The term RNA silencing (RNA interference, RNAi) describes a set of mechanisms that regulate gene expression in eukaryotes. Small interfering RNAs (siRNA) and microRNAs (miRNAs) are two major types of RNAi-associated small RNAs (smRNAs) found in most ...
Ning Xu, Yan Li, Ying-Tao Zhao, Li Guo, Yuan-Yuan Fang, Jian-Hua Zhao, Xiu-Jie Wang, Li Huang, Hui-Shan Guo   +8 more
doaj   +1 more source

Molecular analysis of 3D domain swapping in the acylphosphatase from Escherichia coli

Acta Crystallographica Section D, Volume 82, Issue 4, Page 336-347, April 2026.
Structures of the monomer and intertwined dimer of the acylphosphatase from E. coli shed light on the molecular basis of its 3D domain swapping.Three‐dimensional domain swapping is a mechanism by which proteins form oligomers. At present, the molecular basis that dictates whether some proteins fold in their monomeric form or as intertwined oligomers is
Sergio Martínez-Rodríguez, Jose A. Gavira, M. Carmen Salinas-Garcia, Montserrat Andujar-Sánchez, Ana Camara-Artigas   +4 more
wiley   +1 more source

Cas6 specificity and CRISPR RNA loading in a complex CRISPR-Cas system [PDF]

, 2014
This research was funded in part by Biotechnology and Biological Sciences Research Council [BB/K000314/1]. The APC was paid through RCUK OA block grant funds.CRISPR-Cas is an adaptive prokaryotic immune system, providing protection against viruses and ...
Malcolm F. White, White, Malcolm F, Shirley Graham, Sokolowski, Richard David, Graham, Shirley, Richard D. Sokolowski, White, Malcolm F.; id_orcid, Sokolowski, Richard D.   +7 more
core   +1 more source

The return of metabolism: biochemistry and physiology of glycolysis

Biological Reviews, Volume 101, Issue 2, Page 751-803, April 2026.
ABSTRACT Glycolysis is a fundamental metabolic pathway central to the bioenergetics and physiology of virtually all living organisms. In this comprehensive review, we explore the intricate biochemical principles and evolutionary origins of glycolytic pathways, from the classical Embden–Meyerhof–Parnas (EMP) pathway in humans to various prokaryotic and ...
Nana‐Maria Grüning, Federica Agostini, Camila Caldana, Johannes Hartl, Matthias Heinemann, Markus A. Keller, Jan Lukas Krüsemann, Costanza Lamperti, Carole L. Linster, Steffen N. Lindner, Julia Muenzner, Jens Nielsen, Zoran Nikoloski, Bettina Siebers, Jacky L. Snoep, Hezi Tenenboim, Bas Teusink, Spencer J. Williams, Mirjam M. C. Wamelink, Markus Ralser   +19 more
wiley   +1 more source

The bindosome is a structural component of the Sulfolobus solfataricus cell envelope [PDF]

Extremophiles, 2011
Sugar binding proteins of the thermoacidophile Sulfolobus solfataricus function together with ABC transporters in the uptake of sugars. They are synthesized as precursors with a class III signal peptide that are normally found in archaeal flagellins and bacterial type IV pilins. The functional expression of sugar binding proteins at the cell surface is
Zolghadr, Behnam, Klingl, Andreas, Rachel, Reinard, Driessen, Arnold J. M., Albers, Sonja Verena   +4 more
openaire   +5 more sources

Characterization of a hyperthermophilic sulphur-oxidizing biofilm produced by archaea isolated from a hot spring

Electronic Journal of Biotechnology, 2017
Background: Sulphur-oxidizing microorganisms are widely used in the biofiltration of total reduced sulphur compounds (odorous and neurotoxic) produced by industries such as the cellulose and petrochemical industries, which include high-temperature ...
Emky Valdebenito-Rolack, Nathaly Ruiz-Tagle, Leslie Abarzúa, Germán Aroca, Homero Urrutia   +4 more
doaj   +1 more source

Protein Language Model‐Guided Engineering of a 2,3‐Butanediol Dehydrogenase for the Enantioselective Synthesis of Cyclic α‐Hydroxy Ketones

Advanced Science, Volume 13, Issue 18, 27 March 2026.
A (2R,3R)‐butanediol dehydrogenase from Bacillus subtilis (BsBDH) is engineered for the enantioselective synthesis of 2‐hydroxycyclohexanone. A PASS computational design strategy is proposed to enhance the thermostability of BsBDH. Moreover, ESM‐1v combined with ISM is utilized for enhancing and inverting its stereoselectivity.
Haote Ding, Ling Jiang, Yijia Song, Zhongji Pu, Lirong Yang, Haoran Yu   +5 more
wiley   +1 more source

Transcriptional response to DNA damage in the archaeon Sulfolobus solfataricus [PDF]

Nucleic Acids Research, 2003
Exposure of cells to DNA-damaging agents triggers a complex biological response involving cell cycle arrest and modulation of gene expression. Genomic sequencing has revealed the presence of archaeal genes homologous to components of the eucaryal nucleotide excision repair (NER) pathway, which is involved in the repair of ultraviolet (UV) light-induced
Salerno V, Napoli A, White MF, Rossi M, Ciaramella M   +4 more
openaire   +5 more sources

Control of cyclic oligoadenylate synthesis in a type III CRISPR system

eLife, 2018
The CRISPR system for prokaryotic adaptive immunity provides RNA-mediated protection from viruses and mobile genetic elements. When viral RNA transcripts are detected, type III systems adopt an activated state that licenses DNA interference and synthesis
Christophe Rouillon, Januka S Athukoralage, Shirley Graham, Sabine Grüschow, Malcolm F White   +4 more
doaj   +1 more source

Analysis of nucleotide insertion opposite urea and translesion synthesis across urea by DNA polymerases

Genes and Environment, 2022
Urea (Ua) is produced in DNA as the result of oxidative damage to thymine and guanine. It was previously reported that Klenow fragment (Kf) exo− incorporated dATP opposite Ua, and that DNA polymerase β was blocked by Ua.
Taishu Kawada, Katsuhito Kino, Kyousuke Tokorodani, Ryuto Anabuki, Masayuki Morikawa, Takanobu Kobayashi, Kazuaki Ohara, Takayuki Ohshima, Hiroshi Miyazawa   +8 more
doaj   +1 more source