Results 101 to 110 of about 13,197 (261)

An Autonomously Replicating Transforming Vector for Sulfolobus solfataricus [PDF]

Journal of Bacteriology, 1998
ABSTRACT A plasmid able to transform and to be stably maintained both in Sulfolobus solfataricus and in Escherichia coli was constructed by insertion into an E. coli plasmid of the autonomously replicating sequence of the virus particle SSV1 and a suitable ...
R. Cannio, CONTURSI, PATRIZIA, ROSSI, MOSE', BARTOLUCCI, SIMONETTA   +3 more
openaire   +5 more sources

Regulation of DNA Topology in Archaea: State of the Art and Perspectives

Molecular Microbiology, Volume 123, Issue 3, Page 245-264, March 2025.
DNA topoisomerases are ubiquitous enzymes that play a crucial role in regulating DNA supercoiling which affects fundamental biological processes involving DNA. In this review, we summarize the current understanding of regulation of supercoiling by DNA topoisomerases in the third domain of life, the Archaea, with a particular focus on three key model ...
Paul Villain, Tamara Basta
wiley   +1 more source

Identification of novel components of the Ced and Ups systems in Saccharolobus islandicus REY15A

mLife, Volume 4, Issue 1, Page 17-28, February 2025.
Abstract In Sulfolobales cells, transcription of the Ups (UV‐inducible pili of Sulfolobus) and Ced (Crenarchaeal system for exchange of DNA) genes is highly induced by DNA damage, and the two systems play key roles in pili‐mediated cell aggregation and chromosomal DNA import, respectively.
Pengju Wu, Mengqi Zhang, Yanlu Kou, Shikuan Liang, Jinfeng Ni, Qihong Huang, Yulong Shen   +6 more
wiley   +1 more source

Crystal structure and substrate specificity of the thermophilic serine:pyruvate aminotransferase from Sulfolobus solfataricus. [PDF]

, 2015
Journal ArticleResearch Support, Non-U.S. Gov'tThe three-dimensional structure of the Sulfolobus solfataricus serine:pyruvate aminotransferase has been determined to 1.8 Å resolution.
Bommer, M, Isupov, MN, Littlechild, JA, Sayer, Christopher, Ward, J   +4 more
core   +1 more source

Capturing chromosome conformation in Crenarchaea

Molecular Microbiology, Volume 123, Issue 2, Page 101-108, February 2025.
To fit into cells, chromosomes must be folded. Here we describe how members of the Archaea effect this folding process and how chromosome architecture both influences and is influenced by gene expression. Abstract While there is a considerable body of knowledge regarding the molecular and structural biology and biochemistry of archaeal information ...
Elyza Pilatowski‐Herzing, Rachel Y. Samson, Naomichi Takemata, Catherine Badel, Peter B. Bohall, Stephen D. Bell   +5 more
wiley   +1 more source

The Lrs14 family of DNA‐binding proteins as nucleoid‐associated proteins in the Crenarchaeal order Sulfolobales

Molecular Microbiology, Volume 123, Issue 2, Page 132-142, February 2025.
Crenarchaeal Sulfolobales organize their genome with small, abundant nucleoid‐associated proteins. Here, we shed light on the distinct function of the Lrs14 family of aspecific DNA‐binding proteins, position these members toward the “chromatin organizing” side of the spectrum of DNA binders and propose a regulatory role for Lrs14s in global gene ...
Veerke De Kock, Eveline Peeters, Rani Baes   +2 more
wiley   +1 more source

Genetic tools for Sulfolobus spp.: vectors and first applications [PDF]

, 2018
Sulfolobus species belong to the best-studied archaeal organisms but have lacked powerful genetic methods. Recently, there has been considerable progress in the field of Sulfolobus genetics.
Berkner, Silvia, Lipps, Georg
core  

Structure of a putative NTP pyrophosphohydrolase: YP_001813558.1 from Exiguobacterium sibiricum 255-15. [PDF]

, 2010
The crystal structure of a putative NTPase, YP_001813558.1 from Exiguobacterium sibiricum 255-15 (PF09934, DUF2166) was determined to 1.78 Å resolution. YP_001813558.1 and its homologs (dimeric dUTPases, MazG proteins and HisE-encoded phosphoribosyl ATP ...
Abdubek, Polat, Astakhova, Tamara, Axelrod, Herbert L, Carlton, Dennis, Chen, Connie, Chiu, Hsiu Ju, Clayton, Thomas, Das, Debanu, Deacon, Ashley M, Deller, Marc C, Duan, Lian, Elsliger, Marc André, Ernst, Dustin, Feuerhelm, Julie, Godzik, Adam, Grant, Joanna C, Grzechnik, Anna, Han, Gye Won, Hodgson, Keith O, Jaroszewski, Lukasz, Jin, Kevin K, Johnson, Hope A, Klock, Heath E, Knuth, Mark W, Kozbial, Piotr, Krishna, S Sri, Kumar, Abhinav, Lam, Winnie W, Lesley, Scott A, Marciano, David, McMullan, Daniel, Miller, Mitchell D, Morse, Andrew T, Murzin, Alexey G, Nigoghossian, Edward, Okach, Linda, Reyes, Ron, Rife, Christopher L, Sefcovic, Natasha, Tien, Henry J, Trame, Christine B, van den Bedem, Henry, Weekes, Dana, Wilson, Ian A, Wooley, John, Xu, Qingping, Yeates, Todd O   +46 more
core   +2 more sources

Regulatory Properties of Glutamate Dehydrogenase from Sulfolobus solfataricus

Molecules and Cells, 2000
The purified glutamate dehydrogenase (GDH) from Sulfolobus solfataricus showed remarkable thermostability and retained 90-95% of the initial activity after incubation at -20 degrees C, 4 degrees C, and 25 degrees C for up to 6 months. Unlike mammalian GDHs, the activity of GDH from Sulfolobus solfataricus was not significantly affected by the presence ...
J Y, Ahn, K S, Lee, S Y, Choi, S W, Cho
openaire   +2 more sources

Exploiting Archaeal/Thermostable Enzymes in Synthetic Chemistry: Back to the Future?

ChemCatChem, Volume 16, Issue 21, November 11, 2024.
Thermostable and (hyper)thermophilic enzymes offer important opportunities for innovative, stereoselective and sustainable chemistry transformations. Many of these are archaeal enzymes that have evolved to operate under extreme conditions, including at elevated temperature.
Gaurav P. Kudalkar, Virendra K. Tiwari, David B. Berkowitz   +2 more
wiley   +1 more source