Prediction of mutations on structure primase of the archaeon Sulfolobus solfataricus
All living organisms need a DNA replication mechanism and it has been conserved in the three domains of life throughout evolutionary process. Primase is the enzyme responsible for synthesizing de novo RNA primers in DNA replication. Archaeo-Eukaryotic
Eden Silva e Souza +1 more
doaj +1 more source
An exosome‐like complex in Sulfolobus solfataricus [PDF]
We present the first experimental evidence for the existence of an exosome‐like protein complex in Archaea. In Eukarya, the exosome is essential for many pathways of RNA processing and degradation. Co‐immunoprecipitation with antibodies directed against the previously predicted Sulfolobus solfataricus orthologue of the exosome subunit ribosomal‐RNA ...
Evguenieva-Hackenberg, E. +4 more
openaire +3 more sources
A comprehensive analysis of the importance of translation initiation factors for Haloferax volcanii applying deletion and conditional depletion mutants [PDF]
Translation is an important step in gene expression. The initiation of translation is phylogenetically diverse, since currently five different initiation mechanisms are known.
Gäbel, Katrin +4 more
core +3 more sources
Hot and crispy : CRISPR-Cas systems in the hyperthermophile Sulfolobus solfataricus [PDF]
The CRISPR (clustered regularly interspaced short palindromic repeats) and Cas (CRISPR-associated) genes are widely spread in bacteria and archaea, representing an intracellular defence system against invading viruses and plasmids.
White, Malcolm F., Zhang, Jing
core +2 more sources
The Sulfolobus solfataricus P2 genome project
Over 800 kbp of the 3‐Mbp genome of Sulfolobus solfataricus have been sequenced to date. Our approach is to sequence subclones of mapped cosmids, followed by sequencing directly on cosmid templates with custom primers. Using a prototype automated system for genome‐scale analysis, known as MAGPIE, along with other tools, we have discovered one open ...
Charlebois, RL +4 more
openaire +3 more sources
“Hot standards” for the thermoacidophilic archaeon Sulfolobus solfataricus [PDF]
Within the archaea, the thermoacidophilic crenarchaeote Sulfolobus solfataricus has become an important model organism for physiology and biochemistry, comparative and functional genomics, as well as, more recently also for systems biology approaches. Within the Sulfolobus Systems Biology ("SulfoSYS")-project the effect of changing growth temperatures ...
Zaparty, M. +21 more
openaire +7 more sources
Crenarchaeal biofilm formation under extreme conditions. [PDF]
BACKGROUND: Biofilm formation has been studied in much detail for a variety of bacterial species, as it plays a major role in the pathogenicity of bacteria.
Andrea Koerdt +4 more
doaj +1 more source
Evolutionary rates and gene dispensability associate with replication timing in the Archaeon Sulfolobus islandicus [PDF]
In bacterial chromosomes, the position of a gene relative to the single origin of replication generally reflects its replication timing, how often it is expressed, and consequently, its rate of evolution.
Cooper, Vaughn S. +3 more
core +2 more sources
Glutamate dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus [PDF]
An NAD(P)‐dependent glutamate dehydrogenase was purified to homogeneity from the thermoacidophilic archaebacterium Sulfolobus solfataricus. The enzyme is a hexamer (subunit mass 45 kDa) which dissociates into lower states of association when submitted to gel filtration.
CONSALVI, Valerio +5 more
openaire +4 more sources
Ultrastructural and proteomic evidence for the presence of a putative nucleolus in an Archaeon
Nucleoli are subcellular compartments where transcription and maturation of pre-ribosomal RNAs occur. While the transcription of ribosomal RNAs is common to all living cells, the presence and ultrastructure of nucleoli has been only documented in ...
Parsifal F. Islas-Morales +11 more
doaj +1 more source

