Results 91 to 100 of about 4,890,068 (298)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Investigation of the dynamic charge transport behaviours under electron beam irradiation of advanced insulation materials for aerospace applications

High Voltage
Aerospace dielectric components on spacecrafts are frequently exposed to surface charging. Here the dynamic charging behaviours of advanced aerospace dielectrics under electron beam irradiation are investigated.
Guangyu Sun, Xiong Yang, Wentong An, Kun Huang, Xiaogang Qin, Baipeng Song, Guanjun Zhang   +6 more
doaj   +1 more source

The Effect of Cycling on the State of Health of the Electric Vehicle Battery [PDF]

, 2013
This paper provides an analysis of the experimental results available for lithium ion battery degradation which has been used to create a model of the effect of the identified parameters on the ageing of an EV battery.
Ghanim Putrus, Kotter, Richard, Gillian Lacey, Tianxiang Jiang, Richard Kotter, Putrus, Ghanim, Jiang, Tianxiang, Lacey, Gillian   +7 more
core   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Quantification of surface charging memory effect in ionization wave dynamics. [PDF]

Sci Rep, 2022
Viegas P, Slikboer E, Bonaventura Z, Garcia-Caurel E, Guaitella O, Sobota A, Bourdon A.   +6 more
europepmc   +1 more source

Surface charging, discharging and chemical modification at a sliding contact [PDF]

, 2012
Electrostatic charging, discharging, and consequent surface modification induced by sliding dissimilar surfaces have been studied. The surface-charge related phenomena were monitored by using a home-built capacitive, non-contact electrical probe, and the
S. V. Singh, Kusano, Yukihiro, Singh, Shailendra Vikram, Morgen, Per, P. Morgen, Morgen, Per; id_orcid, P. K. Michelsen, Michelsen, Poul, Y. Kusano   +8 more
core   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source

Instrumentation for Studies of Electron Emission and Charging from Insulators [PDF]

, 2003
Making measurements of electron emission properties of insulators is difficult since insulators can charge either negatively or positively under charge particle bombardment.
Zavyalov, V. V., Dennison, John R., Thomson, C. D.   +2 more
core   +1 more source

Shaping urban traffic patterns through congestion charging: What factors drive success or failure?. [PDF]

Congestion costs are emerging as one of the most important challenges faced by metropolitan planners and transport authorities in first world economies. In US these costs were as high as 78 million dollars in 2005 and are growing due to fast increases in
Daniel Albalate, Germa Bel
core  

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source