Results 91 to 100 of about 5,084,579 (305)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Recursive Time Series Prediction Modeling of Long-Term Trends in Surface Settlement During Railway Tunnel Construction

CivilEng
The surface settlement of railroad tunnels is dynamically updated as the construction progresses, exhibiting complex nonlinear characteristics. The accuracy of the on-site nonlinear regression fitting prediction method needs to be improved.
Feilian Zhang, Qicheng Wei, Zhe Wu, Jiawei Cao, Danlin Jian, Lantian Xiang   +5 more
doaj   +1 more source

A novel combined intelligent algorithm prediction model for the tunnel surface settlement. [PDF]

Sci Rep, 2023
Wang Y, Dai F, Jia R, Wang R, Sharifi H, Wang Z.   +5 more
europepmc   +1 more source

Settlement patterns of the coral Acropora millepora on sediment-laden surfaces

Science of The Total Environment, 2017
Successful recruitment in corals is important for the sustenance of coral reefs, and is considered a demographic bottleneck in the recovery of reef populations following disturbance events. Yet several factors influence larval settlement behaviour, and here we quantified thresholds associated with light attenuation and accumulated sediments on ...
Gerard F. Ricardo, Ross J. Jones, Mikaela Nordborg, Andrew P. Negri   +3 more
openaire   +4 more sources

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Trade dispute settlement mechanisms: the WTO dispute settlement understanding in the wake of the GATT [PDF]

A critical feature of the GATT Uruguay Round negotiations was the establishment of a new and more effective system of dealing with international trade disputes, known as the WTO Dispute Settlement Understanding (DSU). The original GATT dispute settlement
R A Read
core  

Numerical Analysis of Diaphragm Wall Deformation and Surface Settlement Caused by Dewatering and Excavation at Center and End Positions in a Subway Foundation Pit

Buildings
Metro foundation pits are important components of urban infrastructure projects. Dewatering and excavation are essential stages in foundation pit construction; however, this process can significantly induce groundwater drawdown, as well as diaphragm wall
Kaifang Yang, Mingdong Jiang, Minliang Chi, Guohui Feng   +3 more
doaj   +1 more source

Study on the effect of excavation sequence of three-hole shield tunnel on surface settlement and segment deformation. [PDF]

Sci Rep, 2023
Wang Y, Dai F, Ding B, Zhong M, Zhang H.
europepmc   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source