Sperm selection with density gradient centrifugation and swim up: effect on DNA fragmentation in viable spermatozoa. [PDF]
Sci Rep, 2019 Subjects increasing sperm DNA fragmentation (sDF) during Density Gradient Centrifugation (DGC), a common sperm selection procedure in Assisted Reproduction Techniques (ARTs), experience a 50% lower probability of pregnancy. Hence, identification of these
Muratori M +11 more
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Sperm enrichment from poor semen samples by double density gradient centrifugation in combination with swim-up for IVF cycles. [PDF]
Sci Rep, 2020 Sperm preparation in IVF cycles using density gradient centrifugation (DGC) in combination with swim-up (SU) has been widely adopted in reproductive centres worldwide.
Dai X +7 more
europepmc +2 more sources
The motility-based swim-up technique separates bull sperm based on differences in metabolic rates and tail length. [PDF]
PLoS One, 2019 Swim-up is a sperm purification method that is being used daily in andrology labs around the world as a simple step for in vitro sperm selection. This method accumulates the most motile sperm in the upper fraction and leaves sperm with low or no motility
Magdanz V +4 more
europepmc +2 more sources
The Iraqi Journal of Agricultural science, 2021 This study was conducted to investigate the effect of swim-up and glass wool as sperm separation techniques with adding vitamin E and superoxide dismutase (SOD) to Tris extender on improving some post-cryopreserved semen quality for Holstein bulls.
M. S. Hassan, S. M. Eidan
doaj +2 more sources
Effect of zinc on sperm recovered by swim-up. [PDF]
J Assist Reprod GenetZinc is known to influence chromatin stability, motility and protection against oxidative stress. While swim-up remains the preferred method for selecting sperm in Assisted Reproductive Technologies (ART), concerns arise regarding sperm DNA fragmentation associated with this procedure. Given zinc's significant role in protecting sperm DNA integrity and
Faggi M +3 more
europepmc +3 more sources
Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation. [PDF]
Clin Exp Reprod Med, 2017 Objective The aim of this study was to compare the efficacy of swim-up and density gradient centrifugation (DGC) for reducing the amount of sperm with fragmented DNA, sex chromosome aneuploidy, and abnormal chromatin structure. Methods Semen samples were
Kim SW, Jee BC, Kim SK, Kim SH.
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A Comparison of Swim-up and Simple Swim-up Methods for the Extraction of High Motility Sperm
Siriraj Medical Journal, 2020 The objective of this study was to compare two methods for the extraction of high motility sperm; swim-up and simple swim-up technique. The results were comparable in concentration, motility and morphology.
Singpetch Suksompong +4 more
doaj +1 more source
Combination of density gradient centrifugation and swim-up methods effectively decreases morphologically abnormal sperms. [PDF]
J Reprod Dev, 2016 Density gradient centrifugation (DGC) and swim-up techniques have been reported for semen preparation in assisted reproductive techniques in humans.
Yamanaka M +9 more
europepmc +2 more sources
Semen preparation methods and sperm telomere length: density gradient centrifugation versus the swim up procedure. [PDF]
Sci Rep, 2016 Previous studies have shown that both density gradient centrifugation (DGC) and swim up (SU) procedures can select spermatozoa with longer telomeres for assisted reproduction techniques (ART). However, it is unknown which approach is more effective.
Zhao F, Yang Q, Shi S, Luo X, Sun Y.
europepmc +2 more sources
A new micro swim-up procedure for sperm preparation in ICSI treatments: preliminary microbiological testing. [PDF]
JBRA Assist Reprod, 2016 Objective This study aimed to assess the levels of microbial contamination in semen samples before and after the micro swim-up (MSU) procedure in intra-cytoplasmic sperm injection (ICSI). The new method is an upgrade to the classic wash swim-up procedure.
Palini S +8 more
europepmc +2 more sources