Results 51 to 60 of about 29,169 (246)

Numerical study on the influence of pre-swirl angle on internal flow characteristics of centrifugal pumps

AIP Advances, 2022
The effect of inlet pre-swirl on the performance of a centrifugal pump is studied by numerical simulation. The governing equations are Navier–Stokes equations and the shear stress transport k–ω turbulence model.
Y. Wang, W. Xu, H. Yin, Y. Zhang, H.-S. Dou   +4 more
doaj   +1 more source

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Flame–Flame Interactions and Jet–Jet Interactions in Gas Turbine Swirl Combustors

Energies
Annular combustors are widely used in newly developed aero-engines. Nevertheless, the development of annular combustors requires substantial air supplies and high-power heaters during testing, leading to high experimental costs.
Wei Wei, Xin Hui, Xin Xue, Qiang An, Shiyang Yu   +4 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Numerical Investigation of Flow and Flame Structures in an Industrial Swirling Inverse Diffusion Methane/Air Burner

Fire
In this study, a novel gas burner combining air swirl and an inverse diffusion flame (IDF) is designed for industrial applications. Numerical simulations using the Reynolds-averaged Navier–Stokes (RANS) method and simplified reaction mechanisms are ...
Mengwei Sun, Yali Shao, Yu Gong, Chuanyi Xu, Tao Song, Ping Lu, Ramesh K. Agarwal   +6 more
doaj   +1 more source

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

Estudio y caracterización de un quemador establecido por giro [PDF]

, 2008
En esta Tesis doctoral se estudia el campo fluido de un quemador estabilizado por giro de nueva generación. La motivación del estudio se basa en el interés actual de profundizar en el conocimiento de los procesos involucrados para, a medio y largo plazo,
Legrand, Mathieu
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Mass transport using the series of vortex rings with swirl

Journal of Fluid Science and Technology, 2014
Behavior of the series of laminar vortex rings with circumferential flow, so-called swirl, were investigated using flow visualization, to evaluate the transport efficiency of the ejected fluid as vortex rings.
Takashi NAITOH, Nobuyuki OKURA, Osamu SUMITOMO   +2 more
doaj   +1 more source