Results 91 to 100 of about 3,003,336 (196)

Synthetic Nucleotides as Probes of DNA Polymerase Specificity

Journal of Nucleic Acids, 2012
The genetic code is continuously expanding with new nucleobases designed to suit specific research needs. These synthetic nucleotides are used to study DNA polymerase dynamics and specificity and may even inhibit DNA polymerase activity. The availability
Jason M. Walsh, Penny J. Beuning
doaj   +1 more source

Interaction of DNA with groove binding ligands [PDF]

Synthetic molecules that target the major groove in a sequence-selective way are a major goal in molecular medicine. Recently a major step has been taken toward achieving this goal: a novel cylinder has been developed that binds strongly into the major ...
Broome, Mark John
core  

Circular single-stranded DNA as a programmable vector for gene regulation in cell-free protein expression systems

Nature Communications
Cell-free protein expression (CFE) systems have emerged as a critical platform for synthetic biology research. The vectors for protein expression in CFE systems mainly rely on double-stranded DNA and single-stranded RNA for transcription and translation ...
Zhijin Tian, Dandan Shao, Linlin Tang, Zhen Li, Qian Chen, Yongxiu Song, Tao Li, Friedrich C. Simmel, Jie Song   +8 more
doaj   +1 more source

Pioneering artificial cell-like structures with DNA nanotechnology-based liquid-liquid phase separation

Biophysics and Physicobiology
Recent studies have revealed that liquid-liquid phase separation (LLPS) plays crucial roles in various cellular functions. Droplets formed via LLPS within cells, often referred to as membraneless organelles, serve to concentrate specific molecules, thus ...
Yusuke Sato, Masahiro Takinoue
doaj   +1 more source

Suppression of ADP-ribosylation reversal triggers cell vulnerability to alkylating agents

Neoplasia: An International Journal for Oncology Research
The ADP-ribosyl hydrolases PARG and ARH3 counteract PARP enzymatic activity by removing ADP-ribosylation. PARG and ARH3 activities have a synthetic lethal effect; however, the specific molecular mechanisms underlying this response remain unknown.
Rocco Caggiano, Evgeniia Prokhorova, Lena Duma, Kira Schützenhofer, Raffaella Lauro, Giuliana Catara, Rosa Marina Melillo, Angela Celetti, Rebecca Smith, S John Weroha, Scott H Kaufmann, Ivan Ahel, Luca Palazzo   +12 more
doaj   +1 more source

DNA-programmed combinatorial library synthesis.

, 2016
For each of four synthetic steps, the DNA genes were split into 384 sub-pools by hybridization of the codons in one of the coding regions to a spatially arrayed set of complementary oligonucleotides.
Nicolas P. Tilmans (2291161), Patrick O. Brown (5555), Pehr B. Harbury (175843), Casey J. Krusemark (2291164)   +3 more
core   +1 more source

Design and construction of a new Drosophila species, D.synthetica, by synthetic regulatory evolution [PDF]

, 2011
Here, I merge the principles of synthetic biology^1,2^ and regulatory evolution^3-11^ to create a new species^12-15^ with a minimal set of known elements.
Moreno, Eduardo, Eduardo Moreno
core   +1 more source

A lumen-tunable triangular DNA nanopore for molecular sensing and cross-membrane transport

Nature Communications
Synthetic membrane nanopores made of DNA are promising systems to sense and control molecular transport in biosensing, sequencing, and synthetic cells. Lumen-tunable nanopore like the natural ion channels and systematically increasing the lumen size have
Xiaoming Liu, Fengyu Liu, Hemani Chhabra, Christopher Maffeo, Zhuo Chen, Qiang Huang, Aleksei Aksimentiev, Tatsuo Arai   +7 more
doaj   +1 more source

Thermo-Programmed Synthetic DNA-Based Receptors. [PDF]

ACS Nano, 2023
Mariottini D, Idili A, Ercolani G, Ricci F.   +3 more
europepmc   +1 more source

Using Synthetic DNA Libraries to Investigate Chromatin and Gene Regulation. [PDF]

Chromosoma, 2023
Kleinschmidt H, Xu C, Bai L.
europepmc   +1 more source