Results 81 to 90 of about 2,867,164 (299)

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

DNA supercoiling induced by a synthetic polypeptide [PDF]

Biopolymers, 1983
The synthetic protein (Leu67-Lys33)100-Orn20 bound irreversibly to pBR322 circular DNA and induced supercoiling in the DNA. The no. of topoisomers reached an optimum corresponding to a wt.-wt. ratio of 1, then remained approx. const. The presence of a population of different topoisomers probably indicates nonregularity in the topol.
DE SANTIS, Pasquale, M. Falcioni, MOROSETTI, Stefano, SAVINO, Maria   +3 more
openaire   +3 more sources

A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei [PDF]

, 1992
Tissue-specific patterns of methylated deoxycytidine residues in the mammalian genome are preserved by postreplicative methylation of newly synthesized DNA. DNA methyltransferase (MTase) is here shown to associate with replication foci during S phase but
Heinz-Ulrich Weier, Page, A.W., Weier, H.U., Andrea W. Page, Bestor, Timothy H., Bestor, T. H., Heinrich Leonhardt, Page, A. W., Page, Andrea W., Leonhardt, Heinrich, Weier, H. U., Weier, Heinz-Ulrich, Bestor, T.H., Timothy H. Bestor, Leonhardt, H.   +14 more
core   +2 more sources

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source

The extreme C terminus of herpes simplex virus DNA polymerase is crucial for functional interaction with processivity factor UL42 and for viral replication [PDF]

, 1993
The herpes simplex virus DNA polymerase is composed of two subunits, a large catalytic subunit (Pol) and a smaller subunit (UL42) that increases the processivity of the holoenzyme.
Bebrin, William R, Paul Digard, M. Coen, Digard, Paul, Weisshart, Klaus, Klaus Weisshart, Coen, Donald M, William R. Bebrin, Donald   +8 more
core  

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Nucleases sintéticas: caracterização bioquímica e mecanismo de ação sobre DNA [PDF]

, 2006
Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Físicas e Matemáticas. Programa de Pós-Graduação em QuímicaFoi estudada a interação de cinco complexos metálicos de Cu2+ com moléculas de DNA.
Oliveira, Mauricio César Bof de
core  

DNA extraction from formalin-fixed tissue: new light from the Deep-Sea [PDF]

, 2010
DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR
Thatje, S., Johnston, J., Johnston, David, Mackenzie-Dodds, Jackie, Clark, P., Hall, S., Palero, F., Mackenzie-Dodds, J., Hall, Sally, Clark, Paul, Palero, Ferran, Mackenzie Dodds, Jackie, Clark, Paul F., Palero, Ferran ; https://orcid.org/, Thatje, Sven   +14 more
core   +1 more source

SemiSynBio: A new era for neuromorphic computing

Synthetic and Systems Biotechnology
Neuromorphic computing has the potential to achieve the requirements of the next-generation artificial intelligence (AI) systems, due to its advantages of adaptive learning and parallel computing. Meanwhile, biocomputing has seen ongoing development with
Ruicun Liu, Tuoyu Liu, Wuge Liu, Boyu Luo, Yuchen Li, Xinyue Fan, Xianchao Zhang, Wei Cui, Yue Teng   +8 more
doaj   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source