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Evolutionary engineering of Saccharomyces cerevisiae: Crafting a synthetic methylotroph via self-reprogramming. [PDF]
Guo F +11 more
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Increased methane production associated with community shifts towards Methanocella in paddy soils with the presence of nanoplastics. [PDF]
He Z +8 more
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Bioconversion of Methanol by Synthetic Methylotrophy
2021As an important building block in the chemical industry, methanol has become an attractive substrate in biorefinery owing to its abundance and low cost. With the development of synthetic biology, metabolic engineering of non-methylotrophy to construct synthetic methylotrophy has drawn increased attention.
Feng, Guo +6 more
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Synthetic Methylotrophy: A Practical Solution for Methanol-Based Biomanufacturing
Trends in Biotechnology, 2020The increasing availability and affordability of natural gas has renewed interest in using methanol for bioproduction of useful chemicals. Engineering synthetic methylotrophy based on natural or artificial methanol assimilation pathways and genetically tractable platform microorganisms for methanol-based biomanufacturing is drawing particular attention.
Yu Wang +4 more
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Metabolic Engineering, 2020
Escherichia coli is an ideal choice for constructing synthetic methylotrophs capable of utilizing the non-native substrate methanol as a carbon and energy source. All current E. coli-based synthetic methylotrophs require co-substrates. They display variable levels of methanol-carbon incorporation due to a lack of native regulatory control of ...
Julia, Rohlhill +3 more
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Escherichia coli is an ideal choice for constructing synthetic methylotrophs capable of utilizing the non-native substrate methanol as a carbon and energy source. All current E. coli-based synthetic methylotrophs require co-substrates. They display variable levels of methanol-carbon incorporation due to a lack of native regulatory control of ...
Julia, Rohlhill +3 more
openaire +2 more sources
Synthetic methylotrophy in Escherichia coli and its industrial applications
2023Anthropogenic climate change, driven by greenhouse gas emissions, is one of humanity’s major challenges in the 21st century. Fossil-powered chemical pro-duction is a major contributor to global greenhouse gas emissions and is re-sponsible for about 1 billion metric tons of carbon dioxide equivalents annually.
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Foundational Tools for Synthetic Methylotrophy in Saccharomyces cerevisiae
2023The global expansion of biomanufacturing is currently limited by the availability of sugar-based microbial feedstocks. One-carbon feedstocks, like methanol, present an enticing alternative to sugar because they can be produced from organic waste, atmospheric carbon dioxide and hydrocarbons.
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Abstract Bacillus methanolicus represents a thermophilic methylotroph whose methanol utilization depends on plasmid-encoded genes. It serves as a unique model for deciphering plasmid-dependent methylotrophy and an ideal chassis for low-carbon biomanufacturing using CO2-derived C1 substrates. Despite its evolutionary uniqueness and industrial potential,
Pan Liu +14 more
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Pan Liu +14 more
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Synthetic alternatives to Matrigel
Nature Reviews Materials, 2020Elizabeth A Aisenbrey, William L Murphy
exaly

