Yeast methylotrophy: metabolism, gene regulation and peroxisome homeostasis. [PDF]
, 2011 Eukaryotic methylotrophs, which are able to obtain all the carbon and energy needed for growth from methanol, are restricted to a limited number of yeast species.
Oku, Masahide +2 more
core +4 more sources
Biosynthesis of L‐5‐methyltetrahydrofolate by genetically engineered Escherichia coli
Microbial Biotechnology, Volume 15, Issue 11, Page 2758-2772, November 2022., 2022 L ‐5‐Methyltetrahydrofolate (L‐5‐MTHF) is the only biologically active form of folate in the human body. Production of L‐5‐MTHF by using microbes is an emerging consideration for green synthesis. Here, Escherichia coli BL21(DE3) was engineered to increase the production of L‐5‐MTHF. After optimizing the conditions and supplementing exogenous substances,
Yubo Wang +6 more
wiley +1 more source
Guidance for engineering of synthetic methylotrophy based on methanol metabolism in methylotrophy
RSC Advances, 2017 Methanol represents an attractive non-food raw material in biotechnological processes from an economic and process point of view. It is vital to elucidate methanol metabolic pathways, which will help to genetically construct non-native methylotrophs.
Wenming Zhang +8 more
openaire +2 more sources
Improving formaldehyde consumption drives methanol assimilation in engineered E. coli [PDF]
, 2018 Due to volatile sugar prices, the food vs fuel debate, and recent increases in the supply of natural gas, methanol has emerged as a promising feedstock for the bio-based economy.
King, Jason R +4 more
core +2 more sources
A pBBR1‐based vector with IncP group plasmid compatibility for Methylorubrum extorquens
MicrobiologyOpen, Volume 11, Issue 5, October 2022., 2022 The pBBR1 derivative pMis1_1B, which was characterized in this study, represents a novel plasmid for Methylorubrum extorquens, which is compatible with the widely used pCM system. The growth defect caused by the plasmid was found to be enhanced by the presence of a pCM plasmid, but cotransformation could be demonstrated to be possible.
Laura Pöschel +2 more
wiley +1 more source
Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus [PDF]
, 2014 Markert B, Stolzenberger J, Brautaset T, Wendisch VF. Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus. BMC Microbiology. 2014;14(1):
Brautaset, Trygve +3 more
core +2 more sources
Comparative genomics in acid mine drainage biofilm communities reveals metabolic and structural differentiation of co-occurring archaea [PDF]
, 2016 Background Metal sulfide mineral dissolution during bioleaching and acid mine drainage (AMD) formation creates an environment that is inhospitable to most life. Despite dominance by a small number of bacteria, AMD microbial biofilm communities contain a
Banfield, Jillian F. +6 more
core +2 more sources
Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris [PDF]
, 2021 Besides bakers' yeast, the methylotrophic yeast Komagataella phaffii (also known as Pichia pastoris) has been developed into the most popular yeast cell factory for the production of heterologous proteins.
De, Sonakshi +3 more
core +1 more source
Synthetic and Systems Biotechnology, 2019 One-carbon compounds, such as methanol, are becoming potential alternatives to sugars as feedstocks for the biological production of chemicals, fuels, foods, and pharmaceuticals.
Monica I. Espinosa +3 more
doaj +1 more source
Methane monooxygenases: central enzymes in methanotrophy with promising biotechnological applications [PDF]
, 2021 Worldwide, the use of methane is limited to generating power, electricity, heating, and for production of chemicals. We believe this valuable gas can be employed more widely.
Brautaset, Trygve +2 more
core +1 more source