Development of a formaldehyde biosensor with application to synthetic methylotrophy [PDF]
Biotechnology and Bioengineering, 2017 Formaldehyde is a prevalent environmental toxin and a key intermediate in single carbon metabolism. The ability to monitor formaldehyde concentration is, therefore, of interest for both environmental monitoring and for metabolic engineering of native and synthetic methylotrophs, but current methods suffer from low sensitivity, complex workflows, or ...
Benjamin M. Woolston +4 more
core +7 more sources
Synthetic methylotrophic yeasts for the sustainable fuel and chemical production [PDF]
Biotechnology for Biofuels and Bioproducts, 2022 Global energy-related emissions, in particular carbon dioxide, are rapidly increasing. Without immediate and strong reductions across all sectors, limiting global warming to 1.5 °C and thus mitigating climate change is beyond reach.
Vanessa Wegat +2 more
doaj +3 more sources
From a Hetero- to a Methylotrophic Lifestyle: Flash Back on the Engineering Strategies to Create Synthetic Methanol-User Strains [PDF]
Frontiers in Bioengineering and Biotechnology, 2022 Engineering microorganisms to grow on alternative feedstocks is crucial not just because of the indisputable biotechnological applications but also to deepen our understanding of microbial metabolism.
Camille Peiro +3 more
doaj +3 more sources
Increasing lysine level improved methanol assimilation toward butyric acid production in Butyribacterium methylotrophicum [PDF]
Biotechnology for Biofuels and Bioproducts, 2023 Background Methanol, a promising non-food fermentation substrate, has gained increasing interest as an alternative feedstock to sugars for the bio-based production of value-added chemicals.
Jing Wang +7 more
doaj +3 more sources
Toward Methanol-Based Biomanufacturing: Emerging Strategies for Engineering Synthetic Methylotrophy in Saccharomyces cerevisiae [PDF]
ACS Synthetic Biology, 2022 The global expansion of biomanufacturing is currently limited by the availability of sugar-based microbial feedstocks, which require farmland for cultivation and therefore cannot support large increases in production without impacting the human food supply.
Philip A. Kelso +4 more
openaire +3 more sources
The cofactor challenge in synthetic methylotrophy: bioengineering and industrial applications
Current Opinion in Biotechnology, 2023 Methanol is a promising feedstock for industrial bioproduction: it can be produced renewably and has high solubility and limited microbial toxicity. One of the key challenges for its bio-industrial application is the first enzymatic oxidation step to formaldehyde.
Krüsemann, Jan L. +4 more
openaire +5 more sources
Engineering and adaptive laboratory evolution of Escherichia coli for improving methanol utilization based on a hybrid methanol assimilation pathway [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Engineering Escherichia coli for efficient methanol assimilation is important for developing methanol as an emerging next-generation feedstock for industrial biotechnology. While recent attempts to engineer E.
Qing Sun +6 more
doaj +2 more sources
Biosensor-based directed evolution of methanol dehydrogenase from Lysinibacillus xylanilyticus [PDF]
Int J Mol Sci, 2021 Methanol dehydrogenase (Mdh), is a crucial enzyme for utilizing methane and methanol as carbon and energy sources in methylotrophy and synthetic methylotrophy.
S J Yeom +8 more
core +3 more sources
Synthetic and Systems Biotechnology, 2019 One-carbon compounds, such as methanol, are becoming potential alternatives to sugars as feedstocks for the biological production of chemicals, fuels, foods, and pharmaceuticals.
Monica I. Espinosa +3 more
doaj +2 more sources
Frontiers in Bioengineering and Biotechnology, 2020 Bioconversion of C1 chemicals such as methane and methanol into higher carbon-chain chemicals has been widely studied. Methanol oxidation catalyzed by methanol dehydrogenase (Mdh) is one of the key steps in methanol utilization in bacterial methylotrophy.
Jin-Young Lee +19 more
doaj +2 more sources