Results 41 to 50 of about 724 (161)
A novel pair of inducible expression vectors for use in Methylobacterium extorquens [PDF]
, 2013 Background: Due to the ever increasing use of diverse microbial taxa in basic research and industrial settings, there is a growing need for genetic tools to alter the physiology of these organisms. In particular, there is a dearth of inducible expression
Carroll, Sean Michael +3 more
core +1 more source
Methanol-essential growth of Escherichia coli
Nature Communications, 2018 Engineering synthetic methylotrophy remains challenging. Here, the authors engineer a methanol-essential E. coli by an in silico-guided multiple knockout approach and show a laboratory evolved strain can incorporate up to 24% methanol into core ...
Fabian Meyer +5 more
doaj +1 more source
Frontiers in Microbiology, 2017 We describe experiments that follow species dynamics and gene expression patterns in synthetic bacterial communities including species that compete for the single carbon substrate supplied, methane, and species unable to consume methane, which could only
Zheng Yu +3 more
doaj +1 more source
Trace metal requirements for microbial enzymes involved in the production and consumption of methane and nitrous oxide [PDF]
, 2012 Fluxes of greenhouse gases to the atmosphere are heavily influenced by microbiological activity. Microbial enzymes involved in the production and consumption of greenhouse gases often contain metal cofactors.
Glass, Jennifer B., Orphan, Victoria J.
core +3 more sources
Synthetic Methylotrophy: Past, Present, and Future
, 2018 Methane and methanol are regarded as alternative and highly attractive nonfood raw materials for the biotechnology sector. The supply of methane and methanol comes from both fossil and renewable resources, rendering them flexible and sustainable raw materials. Reduced one-carbon (C1) compounds are used by specialized groups of microorganisms, i.e., the
Heux, Stephanie +6 more
openaire +2 more sources
Good Codons, Bad Transcript: Large Reductions in Gene Expression and Fitness Arising from Synonymous Mutations in a Key Enzyme [PDF]
, 2014 Biased codon usage in protein-coding genes is pervasive, whereby amino acids are largely encoded by a specific subset of possible codons. Within individual genes, codon bias is stronger at evolutionarily conserved residues, favoring codons recognized by ...
Agashe, Deepa +3 more
core +1 more source
Effective use of a horizontally-transferred pathway for dichloromethane catabolism requires post–transfer refinement [PDF]
, 2015 When microbes acquire new abilities through horizontal gene transfer, the genes and pathways must function under conditions with which they did not coevolve.
Bringel, Françoise +4 more
core +1 more source
Leaf-FISH : microscale imaging of bacterial taxa on phyllosphere [PDF]
, 2018 © The Author(s), 2018. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Microbiology 8 (2018): 2669, doi:10.3389/fmicb.2017.02669.Molecular methods for microbial
Peredo, Elena L., Simmons, Sheri L.
core +2 more sources
Biotechnology and Bioengineering, 2020 AbstractSynthetic methylotrophy aims to engineer methane and methanol utilization pathways in platform hosts like Escherichia coli for industrial bioprocessing of natural gas and biogas. While recent attempts to engineer synthetic methylotrophs have proved successful, autonomous methylotrophy, that is, the ability to utilize methane or methanol as sole
Robert Kyle Bennett +5 more
openaire +3 more sources
The Role of Bacillithiol in Gram-Positive Firmicutes [PDF]
, 2018 Significance: Since the discovery and structural characterization of bacillithiol (BSH), the biochemical functions of BSH-biosynthesis enzymes (BshA/B/C) and BSH-dependent detoxification enzymes (FosB, Bst, GlxA/B) have been explored in Bacillus and ...
Antelmann, Haike +3 more
core +1 more source