Results 211 to 220 of about 819,635 (326)

rDNA Copy Number Variation and Methylation During Normal and Premature Aging

Aging Cell, Volume 24, Issue 5, May 2025.
In > 160 healthy individuals the absolute rDNA copy number (CN) in blood ranged from approximately 240 to 900. The number of active copies with a completely unmethylated promoter varied from 40 to 120, independent of absolute CN. Promoter methylation is a compensation mechanism for absolute CN variation. Aging was associated with a loss of unmethylated
Alva B. C. Geisen, Natalia Santana Acevedo, Junko Oshima, Marcus Dittrich, Ramya Potabattula, Thomas Haaf   +5 more
wiley   +1 more source

Comparison of Commercially Available Thermostable DNA Polymerases with Reverse Transcriptase Activity in Coupled Reverse Transcription Polymerase Chain Reaction Assays. [PDF]

Methods Protoc
Smirnova EV, Blagodatskikh KA, Barsova EV, Varlamov DA, Kramarov VM, Ignatov KB.   +5 more
europepmc   +1 more source

Development of a Short Telomere Zebrafish Model for Accelerated Aging Research and Antiaging Drug Screening

Aging Cell, EarlyView.
Larvae derived from a cross between tert deficient and haploinsufficient fish show a premature aging phenotype, characterized by telomere attrition and early death. The ST2 larval model is a valuable tool for rapid screening of antiaging molecules. Given that organogenesis occurs within the first 48 h, this model also allows for the conduction of aging
David Hernández‐Silva, María D. López‐Abellán, Francisco J. Martínez‐Navarro, Jesús García‐Castillo, María L. Cayuela, Francisca Alcaraz‐Pérez   +5 more
wiley   +1 more source

Linker Design Principles for the Precision Targeting of Oncogenic G-Quadruplex DNA with G4-Ligand-Conjugated Oligonucleotides. [PDF]

Bioconjug Chem
Abrahamsson A, Berner A, Golebiewska-Pikula J, Chaudhari N, Keskitalo E, Lindgren C, Chmielewski MK, Wanrooij S, Chorell E.   +8 more
europepmc   +1 more source

Negative Effect of Gst‐35 on the Health Span of Caenorhabditis elegans Through Lysosomal Dysfunction via the Pmk‐1 and Skr Genes

Aging Cell, EarlyView.
The overexpression of gst‐35, the mammalian orthologous genes GSTA1, GSTA2, and GSTA3, induces lysosomal dysfunction through the genes pmk‐1 and skr in C. elegans. This overexpression not only promotes an in vivo inflammatory response and accumulation of reactive oxygen species but also significantly shortens the nematode lifespan; inhibits its ...
Yehui Gao, Xinyun Zhang, Congmin Wei, Hongru Lin, Mengchen Wu, Botian Ma, Jinyun Jiang, Shan Li, Hongbing Wang   +8 more
wiley   +1 more source

Sweet enhancers of polymerase chain reaction. [PDF]

PLoS One
Xie B, Chen J, Wang Z, Yin Q, Dai ZM.
europepmc   +1 more source

Footprinting of DNA-binding proteins in intact cells [PDF]

, 1993
Becker, Peter B., Schütz, Günther, Weih, Falk, Wu, Ray   +3 more
core   +1 more source

Immunosenescence Profile Is Associated With Increased Susceptibility to Severe COVID‐19

Aging Cell, EarlyView.
(A) A previous immunosenescence profile in individuals infected with SARS‐CoV2 creates a permissive environment for progression to severe COVID‐19: higher inflammaging, accumulation of exhausted/senescent T cells, an oligoclonal B‐cell repertoire, and accelerated epigenetic age.
Lucas Haniel A. Ventura, Lícia Torres, Giovanna Caliman Camatta, Jofer Zamame, Monique Macedo Coelho, Cecília Horta Ramalho‐Pinto, João Gervazio, Felipe Caixeta, Leandro Nascimento, Mariana Almeida Oliveira, Vinícius Dantas Martins, Marcos Felipe Oliveira, Murilo Soares Costa, Hugo Itaru Sato, Henrique Cerqueira Guimarães, Rafael Calvão Barbuto, Ana Paula Rocha Veiga, Najara Ataíde, Gabriela Prandi Caetano, Sarah Rangon, Mauro Lúcio O. Júnior, Fernanda Calvo Fortes, Luciana Zuccherato, Elaine Speziali, Olindo Assis Martins‐Filho, Verônica Coelho, Roberto Avritchir, Rafael Souza, Marina Ayupe, Caio Loureiro, Maria Eduarda Passos, Ana Clara Mota Neves, Pauline Leite, Santuza Maria Ribeiro Teixeira, Unaí Tupinambás, Liza Figueiredo Felicori, Gabriela Silveira‐Nunes, Tatiani Uceli Maioli, Denise Morais Fonseca, Andrea Teixeira‐Carvalho, Ana Maria Caetano Faria   +40 more
wiley   +1 more source

Precise and Accurate DNA-3'/5-Ends Polishing with Thermus thermophilus Phage vb_Tt72 DNA Polymerase. [PDF]

Int J Mol Sci
Dorawa S, Kaczorowski T.
europepmc   +1 more source

hTERT Increases TRF2 to Induce Telomere Compaction and Extend Cell Replicative Lifespan

Aging Cell, EarlyView.
In contrast to the dogma that a subset of short telomeres initiates senescence, hTERT variants unable to maintain telomere length block senescence by stabilizing TRF2 via inhibiting upstream E3 ubiquitin ligases. This noncanonical hTERT function also compacts telomeres and blocks senescence‐associated DNA damage signaling. Cancer cells expressing hTERT
Nancy Adam, Yang Yang, Mahbod Djamshidi, Sara Seifan, Nicholas S. Y. Ting, Joel Glover, Nicolas Touret, Paul M. K. Gordon, K. V. Vineetha Warriyar, Hokan Krowicki, Christine Kim Garcia, Sharon A. Savage, Aaron A. Goodarzi, Duncan M. Baird, Tara L. Beattie, Karl Riabowol   +15 more
wiley   +1 more source