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A high diversity naïve variable new antigen receptor, vNAR, phage library for rapid nanobody discovery across diverse antigens. [PDF]
Kumar V +19 more
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Development of a Novel, Non-Invasive Saliva Sampling Method for the Detection of Bovine Respiratory Viruses. [PDF]
Baumann S +6 more
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Development of a Multiplex RT-PCR Assay for Simultaneous Detection of <i>Velarivirus arecae</i>, <i>Arepavirus arecae</i> and <i>Arepavirus arecamaculatum</i>. [PDF]
Sun K, Zhang L, Li Z, Zhao P, Wan S.
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Dideoxy Sequencing Reactions Using Taq Polymerase
2003Tuq DNA polymerase is a highly stable polymerase isolated from the thermophilic organism Thermus aquaticus. Because of its unique properties this enzyme has been extensively used for amplification of DNA fragments by the polymerase chain reaction (PCR).
Arigoni, Fabrizio +1 more
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NaOH Treatment to Neutralize Inhibitors of Taq Polymerase
Journal of Forensic Sciences, 1999Abstract The introduction of polymerase chain reaction (PCR) into the forensic field has greatly extended the ability to analyze DNA from small or degraded samples. However, one significant problem with PCR analysis is the sensitivity of Taq Polymerase to inhibitors found in many substrates commonly encountered with evidentiary materials.
M T, Bourke +3 more
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Structure of Taq polymerase with DNA at the polymerase active site
Nature, 1996The DNA polymerase from Thermus aquaticus (Taq polymerase) is homologous to Escherichia coli DNA polymerase I (Pol I) and likewise has domains responsible for DNA polymerase and 5' nuclease activities. The structures to the polymerase domains of Taq polymerase and of the Klenow fragment (KF) of Pol I are almost identical, whereas the structure of a ...
S H, Eom, J, Wang, T A, Steitz
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1989
The polymerase chain reaction (PCR) method for amplifying selectively discrete segments of DNA has found wide-spread applications in molecular biology, due in part, to the substitution of a thermostable DNA polymerase isolated from Thermus aquaticus (Taq)1 for the previously used E.
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The polymerase chain reaction (PCR) method for amplifying selectively discrete segments of DNA has found wide-spread applications in molecular biology, due in part, to the substitution of a thermostable DNA polymerase isolated from Thermus aquaticus (Taq)1 for the previously used E.
openaire +1 more source
Taq DNA Polymerase Mutants and 2′-Modified Sugar Recognition
Biochemistry, 2015Chemical modifications to DNA, such as 2' modifications, are expected to increase the biotechnological utility of DNA; however, these modified forms of DNA are limited by their inability to be effectively synthesized by DNA polymerase enzymes.
Hayley J, Schultz +9 more
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