Results 251 to 260 of about 166,603 (275)

A high diversity naïve variable new antigen receptor, vNAR, phage library for rapid nanobody discovery across diverse antigens. [PDF]

open access: yesJ Biol Chem
Kumar V   +19 more
europepmc   +1 more source

Development of a Novel, Non-Invasive Saliva Sampling Method for the Detection of Bovine Respiratory Viruses. [PDF]

open access: yesVet Sci
Baumann S   +6 more
europepmc   +1 more source

Dideoxy Sequencing Reactions Using Taq Polymerase

2003
Tuq DNA polymerase is a highly stable polymerase isolated from the thermophilic organism Thermus aquaticus. Because of its unique properties this enzyme has been extensively used for amplification of DNA fragments by the polymerase chain reaction (PCR).
Arigoni, Fabrizio   +1 more
openaire   +3 more sources

NaOH Treatment to Neutralize Inhibitors of Taq Polymerase

Journal of Forensic Sciences, 1999
Abstract The introduction of polymerase chain reaction (PCR) into the forensic field has greatly extended the ability to analyze DNA from small or degraded samples. However, one significant problem with PCR analysis is the sensitivity of Taq Polymerase to inhibitors found in many substrates commonly encountered with evidentiary materials.
M T, Bourke   +3 more
openaire   +2 more sources

Structure of Taq polymerase with DNA at the polymerase active site

Nature, 1996
The DNA polymerase from Thermus aquaticus (Taq polymerase) is homologous to Escherichia coli DNA polymerase I (Pol I) and likewise has domains responsible for DNA polymerase and 5' nuclease activities. The structures to the polymerase domains of Taq polymerase and of the Klenow fragment (KF) of Pol I are almost identical, whereas the structure of a ...
S H, Eom, J, Wang, T A, Steitz
openaire   +2 more sources

Taq DNA Polymerase

1989
The polymerase chain reaction (PCR) method for amplifying selectively discrete segments of DNA has found wide-spread applications in molecular biology, due in part, to the substitution of a thermostable DNA polymerase isolated from Thermus aquaticus (Taq)1 for the previously used E.
openaire   +1 more source

Taq DNA Polymerase Mutants and 2′-Modified Sugar Recognition

Biochemistry, 2015
Chemical modifications to DNA, such as 2' modifications, are expected to increase the biotechnological utility of DNA; however, these modified forms of DNA are limited by their inability to be effectively synthesized by DNA polymerase enzymes.
Hayley J, Schultz   +9 more
openaire   +2 more sources

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