Results 301 to 310 of about 819,635 (326)

Magnetic silica nanoparticle–Taq polymerase hybrids for multiple uses in polymerase chain reaction

, 2015
Enzyme-magnetic nanoparticle hybrids have been employed as a strategy to improve the biocatalytic usability of biological molecules. For this purpose, we synthesized magnetic core–silica shell nanoparticles for direct one-step easy immobilization of Taq ...
V. Ozalp, G. Bayramoglu, M. Arica
semanticscholar   +1 more source

Peptide nucleic acid as a template for Taq DNA polymerase

Biochemical and Biophysical Research Communications, 2021
Peptide nucleic acid (PNA), an artificial DNA analog, comprises a purine or pyrimidine base and a pseudo-peptide backbone instead of deoxyribose-phosphate. PNA has been found to have stronger adhesion and higher stability in binding to its complementary DNA than deoxyribose-phosphate.
openaire   +2 more sources

Carboxyflavins, novel inhibitors of Taq DNA polymerase.

International Journal of Molecular Medicine, 1998
Carboxyflavins were found to be potent selective inhibitors of Taq DNA polymerase in a polymerase chain reaction. The inhibitions were dose-dependent, and complete inhibitions were observed at the concentration of 3.0 microM. Carboxyflavins were much less, or not sensitive to the DNA polymerases tested such as calf thymus DNA polymerase alpha, rat DNA ...
Kosaku Hirota, Magoichi Sako, Takamasa Ueno, Tsuyoshi Fujita, Yoshiyuki Mizushina, Kengo Sakaguchi, Yuso Goto, Hideya Hayashi, Yoshiaki Isobe   +8 more
openaire   +3 more sources

Dideoxy Sequencing Reactions Using Taq Polymerase

2003
Tuq DNA polymerase is a highly stable polymerase isolated from the thermophilic organism Thermus aquaticus. Because of its unique properties this enzyme has been extensively used for amplification of DNA fragments by the polymerase chain reaction (PCR).
Arigoni, Fabrizio, Kaminski, Pierre-Alexandre   +1 more
openaire   +5 more sources

Alternative dideoxy sequencing of double-stranded DNA by cyclic reactions using Taq polymerase.

DNA and Cell Biology, 1991
The polymerase chain reaction (PCR) is a technique to amplify a specific DNA sequence millions of times. The thermostable enzyme Taq polymerase allows this procedure to take place under conditions of high specificity and automatization.
J. S. Lee
semanticscholar   +1 more source

Taq Polymerase

Catalysis from A to Z, 2012
P. Sears
openaire   +2 more sources

Experimental procedures comparing the activity of different Taq polymerases

Forensic Science International, 2004
Forensic investigations involve several scientific branches among which biological analyses are much more frequently requested as a consequence of their importance and great versatility towards most of the traces found on the crime scene. Biological analyses are lead in subsequent steps: extraction, amplification and STR typing of the specimens ...
D. Di Martino, D. Piscitello, S. Spitaleri, L. Saravo   +3 more
openaire   +3 more sources

Effects of agar and agarose on VentTM DNA polymerase and Taq DNA polymerase

Biotechnology Letters, 1995
We have studied the effects of agar and agarose on Vent™ DNA polymerase and Taq DNA polymerase. Agar strongly inhibited Vent™ DNA polymerase but only moderately inhibited Taq DNA polymerase. Such a difference may be due to the fact that the two polymerases belong to different structural families.
Cheuk Yu Lee, Stephen Kwok-Wing Tsui, Mary M.Y. Waye   +2 more
openaire   +2 more sources

Novel antiproliferative analogs of the Taq DNA polymerase inhibitor catalpol

Bioorganic & Medicinal Chemistry Letters, 2007
The naturally occurring iridoid catalpol (1) is a Taq DNA polymerase inhibitor. However, its poor lipophilicity might account for the lack of biological activity against human solid tumor cell lines. The traditional prodrug approach by means of peracetylation of the free hydroxyl groups led to a compound, which showed a marginal growth inhibition ...
Celina García, José M. Padrón, Leticia G. Leon, Carlos E. Tonn, Carlos R. Pungitore, Víctor S. Martín   +5 more
openaire   +3 more sources

Characterization of contaminating DNA in Taq polymerase which occurs during amplification with a primer set for Legionella 5S ribosomal RNA.

Molecular and Cellular Probes, 1994
An amplification product that occurred in negative controls of a PCR using a primer system for Legionella 55 ribosomal RNA was characterized by direct sequencing. The amplification product did not hybridize to a Legionella specific oligonucleotide.
Matthias Maiwald, H. Ditton, H. Sonntag, M. von Knebel Doeberitz   +3 more
semanticscholar   +1 more source