Results 81 to 90 of about 4,123,279 (296)

Development of SYK NanoBRET cellular target engagement assays for gain–of–function variants

Frontiers in Chemical Biology
Spleen tyrosine kinase (SYK) is a non-receptor tyrosine kinase that is activated by phosphorylation events downstream of FcR, B-cell and T-cell receptors, integrins, and C-type lectin receptors. When the tandem Src homology 2 (SH2) domains of SYK bind to
Jacob L. Capener, James D. Vasta, Vittorio L. Katis, Ani Michaud, Michael T. Beck, Sabrina C. D. Daglish, Sarit Cohen-Kedar, Sarit Cohen-Kedar, Efrat Shaham Barda, Efrat Shaham Barda, Stefanie Howell, Iris Dotan, Iris Dotan, Matthew B. Robers, Alison D. Axtman, Frances M. Bashore   +15 more
doaj   +1 more source

Target engagement: Shining a light.

Nature chemical biology, 2017
The ability to measure the binding of a compound to its intended target in live cells or tissue is a critical parameter for drug discovery. A new method using polarized light microscopy adds to the current toolbox by enabling monitoring of target engagement in vitro and in vivo at single-cell resolution.
openaire   +2 more sources

Community engagement in rural-remote and Indigenous local government [PDF]

, 2012
This study aims to further explore this priority capacity building issue to provide insight to the community engagement practices and challenges of rural-remote and Indigenous local government in Australia for the purpose of identifying some practical ...

core  

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Lived Experience Engagement

The Amplify Engagement Report presents findings from the Children with Incarcerated Parents (CHIRP) study, which aims to estimate the number of children affected by parental incarceration across five Canadian provinces (British Columbia, Alberta ...
Engagement, Amplify
core   +1 more source

Digitalisierung, Klimaschutz und Engagement

, 2022
Herr Kolain hielt den Vortrag online über zoom bei der BBE-Arbeitsgruppe "Digitalisierung und Bürgerschaftliches Engagement" am 02.02 ...
Kolain, Michael
core   +1 more source

Office for Public Engagement: Student Experiences

, 2008
University of Minnesota: Office for Public Engagement. (2008). Office for Public Engagement: Student Experiences.
University of Minnesota: Office for Public Engagement
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Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Stability-based approaches in chemoproteomics

Expert Reviews in Molecular Medicine
Target deconvolution can help understand how compounds exert therapeutic effects and can accelerate drug discovery by helping optimise safety and efficacy, revealing mechanisms of action, anticipate off-target effects and identifying opportunities for ...
Amy L. George, Maria Emilia Dueñas, José Luis Marín-Rubio, Matthias Trost   +3 more
doaj   +1 more source

Appendices for 2002 Civic Engagement Final Report

, 2002
University of Minnesota: Office for Public Engagement. (2002). Appendices for 2002 Civic Engagement Final Report.
University of Minnesota: Office for Public Engagement
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